New ex vivo model of corneal endothelial phacoemulsification injury and rescue therapy with mesenchymal stromal cell secretome

Rouhbakhshzaeri, Majid; Rabiee, Behnam; Azar, Nathalie F.; Ghahari, Elham; Putra, Ilham; Eslani, Medi; Djalilian, Ali R.

doi:10.1016/j.jcrs.2018.09.030

Cited by 8 publications

(13 citation statements)

References 44 publications

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“…; Rouhbakhshzaeri et al. ). Current literature is not completely congruent on this aspect and ongoing investigations are further addressing this issue (Frohn et al.…”

Section: Discussionmentioning

confidence: 99%

“…pupil's centre or off‐centre) (Rouhbakhshzaeri et al. ). Moreover, staining and examination of the CEC was performed after only 4 hr of incubation, which is only useful to assess necrotic cell death.…”

Section: Discussionmentioning

confidence: 99%

“…However, this completely neglects apoptotic cell death processes (Rouhbakhshzaeri et al. ). In our model, CECs were allowed to reorganize and cover damaged areas – comparable to the in vivo situation – over a period of 15 days.…”

Section: Discussionmentioning

confidence: 99%

“…There is no precise information on the size of the analysed endothelial area and the phaco tip's exact position during phacoemulsification (i.e. pupil's centre or off-centre) (Rouhbakhshzaeri et al 2018). Moreover, staining and examination of the CEC was performed after only 4 hr of incubation, which is only useful to assess necrotic cell death.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, staining and examination of the CEC was performed after only 4 hr of incubation, which is only useful to assess necrotic cell death. However, this completely neglects apoptotic cell death processes (Rouhbakhshzaeri et al 2018). In our model, CECs were allowed to reorganize and cover damaged areascomparable to the in vivo situationover a period of 15 days.…”

Section: Discussionmentioning

confidence: 99%

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Effects of ultrasound energy on the porcine corneal endothelium – Establishment of a phacoemulsification damage model

Kunzmann

Wenzel

Bartz‐Schmidt

et al. 2019

Acta Ophthalmologica

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Purpose The purpose of this study was to establish a standardized in vitro phacoemulsification damage model for future investigations of the effects of phacoemulsification, surgical devices, protective ophthalmic viscoelastic devices (OVDs), irrigation solutions and other aspects related to cataract phacoemulsification surgery on the corneal endothelium using porcine eyes. Methods Thirty‐four porcine eyes were randomly assigned to three groups (phacoemulsification (n = 13), irrigation (n = 9), control (n = 12)). A total of 5 min of ultrasound energy with intermittent irrigation/aspiration was applied in the eyes of the phacoemulsification group. The eyes of the irrigation group received the identical treatment, but without the application of ultrasound energy. The control group was left untreated. All eyes were then prepared to split corneal buttons followed by 15 days of cultivation. Endothelial cell density (ECD) was assessed blinded on day 15. Results Endothelial cell density declined significantly more until day 15 in the phacoemulsification group (2567 ± 317/267 cells/mm² (median ± 25%/75%‐quartiles), −32.5 ± 7.0/6.4%) compared to the irrigation (3450 ± 350/383 cells/mm², −11.8 ± 5.3/2.6%; p < 0.001) and the control group (3650 ± 288/258 cells/mm², −10.2 ± 3.2/4.6%; p < 0.001). Conclusion The phacoemulsification damage model presented in this study is sensitive to phacoemulsification energy and may reliably be used to investigate various factors involved in phacoemulsification with regard to their influence on corneal endothelial cells. This method is able to replace animal experiments or in vitro cell culture experiments that often do not translate well to the in vivo situation in humans.

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“…; Rouhbakhshzaeri et al. ). Current literature is not completely congruent on this aspect and ongoing investigations are further addressing this issue (Frohn et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of ultrasound energy on the porcine corneal endothelium – Establishment of a phacoemulsification damage model

Kunzmann

Wenzel

Bartz‐Schmidt

et al. 2019

Acta Ophthalmologica

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The Effect of Mesenchymal Stem Cell Secretome on Corneal Endothelial Cell Preservation in an Oxidative Injury Model

Rabiee

Bheri

Farooq

et al. 2020

Cornea

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Purpose: To describe a reproducible oxidative injury model in ex vivo porcine corneas and to investigate the effects of corneal mesenchymal stem cell (Co-MSC) secretome and specific factors on the preservation of corneal endothelium after oxidative injury. Methods: Porcine corneas underwent vital staining with trypan blue and alizarin red with different concentration and time points. Ex vivo porcine corneas were exposed (endothelial side) to varied concentrations of hydrogen peroxide. After injury, 3 groups of 5 corneas underwent treatment with secretome from either a wild-type (WT) murine Co-MSC, a pigment epithelium derived factor (PEDF) knock out (K/O) murine Co-MSC, or basal media for 4 hours at 37°C. The viability of the endothelium was evaluated using the optimized vital staining protocol. Results: The optimal vital staining was achieved with 0.4% trypan blue for 60 seconds and 0.5% alizarin red for 90 seconds. The optimal oxidative injury (for consistency and level of damage) was obtained with 1% hydrogen peroxide for 15 seconds. Treatment with both WT Co-MSC and PEDF K/O Co-MSC secretome significantly reduced the endothelial damage compared with control (17.2% ± 10.0%, 33.5% ± 11.6%, and 68% ± 17%, respectively, P < 0.01). The WT Co-MSC secretome was significantly more effective compared with PEDF K/O Co-MSC secretome (P < 0.05). Conclusions: A reproducible model of vital staining and oxidative injury is described for studying porcine corneal endothelial survival. Our results demonstrate a beneficial role of a corneal MSC secretome in reducing oxidative damage to the corneal endothelium. In addition, it suggests a potential role for PEDF in this process.

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Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

Guo

Zhang

et al. 2020

Clin Exp Pharma Physio

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Oxidative stress is thought to play an important role in the occurrence and development of Alzheimer's disease (AD) and antioxidants may delay or even treat AD. Oleanolic acid (OA) exhibits antioxidant properties against many diseases. However, its effects on oxidative stress in AD remain unclear. Here, we explored the role and mechanism of action of OA in N2a/APP695swe cells exposed to oxidative stress. The cells were incubated with different concentrations of OA (0, 5, 8, 10, 15, and 25 μmol/L) for 24 hours. Higher concentrations of OA (10, 15, and 25 μmol/L) significantly suppressed the apoptosis, caspase‐3 activity, reactive oxygen species level, and β amyloid (Aβ) content and increased the viability of these cells. OA (10 μmol/L) also increased the expression of stanniocalcin‐1 (STC‐1) and uncoupling protein‐2 (UCP2) in N2a/APP695swe cells. STC‐1 interference markedly reversed the effect of OA on UCP2, indicating that OA may regulate UCP2 expression in N2a/APP695swe cells via STC‐1. Moreover, UCP2 inhibition significantly reversed the OA‐mediated effects on cell viability, caspase‐3 activity, reactive oxygen species, and Aβ level. Thus, OA regulates UCP2 expression via STC‐1 to alleviate oxidative stress and Aβ level in N2a/APP695swe cells.

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New ex vivo model of corneal endothelial phacoemulsification injury and rescue therapy with mesenchymal stromal cell secretome

Abstract: To develop a reproducible ex vivo model of corneal endothelial cell injury using phacoemulsification in porcine eyes and evaluate the effects of mesenchymal stromal cell secretome in this injury model.

Cited by 8 publications

References 44 publications

Effects of ultrasound energy on the porcine corneal endothelium – Establishment of a phacoemulsification damage model

Effects of ultrasound energy on the porcine corneal endothelium – Establishment of a phacoemulsification damage model

The Effect of Mesenchymal Stem Cell Secretome on Corneal Endothelial Cell Preservation in an Oxidative Injury Model

Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

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