New Evidence for Antimicrobial-Resistant Gonorrhea Control Programs: Lessons Learned from the SURRG Project

Barbee, Lindley A

doi:10.1097/olq.0000000000001523

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…However, no detaled inhibition data with many other classes of inhibitors, including phenols, are available so far in the literature for many of these enzymes, among which NgCAα and VchCAα belong 22 , 24 . The first of this enzyme was recently validated 21 as a potential drug target for developing antibiotics able to reverse or at least to alleviate the extensive drug resistance phenomenon that has emerged for this N. gonorrhoeae 25 . Although phenols usually act as weaker CAIs compared to other classes such as the sulphonamides or the sulfamates, we decided to investigate here the susceptibility of these enzymes to inhibition by a series of simple phenols and phenolic acids, of types 1–22 ( Table 1 ) investigated earlier for their interaction with the human CA isoforms 4 , 6 .…”

Section: Resultsmentioning

confidence: 99%

Inhibition studies of bacterial α-carbonic anhydrases with phenols

Giovannuzzi

Hewitt

Nocentini

et al. 2022

Journal of Enzyme Inhibition and Medicinal Chemistry

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The α-class carbonic anhydrases (CAs, EC 4.2.1.1) from the bacterial pathogens Neisseria gonorrhoeae (NgCAα) and Vibrio cholerae (VchCAα) were investigated for their inhibition by a panel of phenols and phenolic acids. Mono-, di- and tri-substituted phenols incorporating additional hydroxyl/hydroxymethyl, amino, acetamido, carboxyl, halogeno and carboxyethenyl moieties were included in the study. The best NgCAα inhibitrs were phenol, 3-aminophenol, 4-hydroxy-benzylalcohol, 3-amino-4-chlorophenol and paracetamol, with K I values of 0.6–1.7 µM. The most effective VchCAα inhibitrs were phenol, 3-amino-4-chlorophenol and 4-hydroxy-benzyl-alcohol, with K I values of 0.7–1.2 µM. Small changes in the phenol scaffold led to drastic effects on the bacterial CA inhibitory activity. This class of underinvestigated bacterial CA inhibitors may thus lead to effective compounds for fighting drug resistant bacteria.

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Section: Resultsmentioning

confidence: 99%

Inhibition studies of bacterial α-carbonic anhydrases with phenols

Giovannuzzi

Hewitt

Nocentini

et al. 2022

Journal of Enzyme Inhibition and Medicinal Chemistry

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Application of Isothermal Signal Amplification Technique in the Etiological Diagnosis of Gonorrhea and Drug Resistance Gene Detection

Chen

Zhang

et al. 2022

Computational and Mathematical Methods in Medicine

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Background: Isothermal signal amplification technique is developed based on the rolling ring amplification mechanism of cyclic DNA molecules in nature. This technique plays an extremely beneficial role in gonorrhea pathogen identification and drug resistance gene detection. Aims: This study analyzes the isothermal signal amplification techniques in the etiological diagnosis of gonorrhea and drug resistance gene detection. Materials and Methods: Urethral, cervical secretion, or prostatic fluid samples from 322 cases of gonorrhea collected from January 2018 to December 2021 at the STD clinic of our hospital dermatology department were selected for direct smear examination and gonococcal culture examination; DNA was extracted from urethral, cervical secretion, or prostatic fluid samples and then used for pathogen identification by SAT assay and rolling loop nucleic acid amplification technique, smear examination and pathogen culture examination methods, SAT assay, and isothermal signal amplification technique for comparative sensitivity and specificity analysis. Results: The highest rate of gonorrhea positivity was for the urine rolling loop nucleic acid amplification technique, followed by the swab rolling loop nucleic acid amplification technique, and the lowest rate of gonorrhea positivity was for the urine SAT test. The difference in the positivity rate between the two urine testing methods was statistically significant ( P < 0.05 ). The highest sensitivity of the urine rolling loop nucleic acid amplification technique method for the detection of gonorrhea pathogens and the lowest sensitivity of the urine SAT method were statistically significant ( P < 0.01 ). The differences in sensitivity and specificity between the swab rolling loop nucleic acid amplification technique and the swab SAT method were not statistically significant ( P > 0.05 ). ROC curves were plotted based on sensitivity and specificity, with swab SAT assay ( A U C = 0.998 ) > rolling loop nucleic acid amplification technique ( A U C = 0.981 ). Comparing the negative rates of urine and swab rolling loop nucleic acid amplification technique and urine SAT assay, the differences were not statistically significant ( P > 0.05 ). Conclusion: The isothermal signal amplification technique improves the shortcomings of gonorrhea pathogen identification means and drug resistance gene detection methods, with good detection sensitivity and specificity, simple operation, low price, and easy promotion, which has obvious advantages in clinical applications and epidemiological studies.

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New Evidence for Antimicrobial-Resistant Gonorrhea Control Programs: Lessons Learned from the SURRG Project

Cited by 2 publications

References 22 publications

Inhibition studies of bacterial α-carbonic anhydrases with phenols

Inhibition studies of bacterial α-carbonic anhydrases with phenols

Application of Isothermal Signal Amplification Technique in the Etiological Diagnosis of Gonorrhea and Drug Resistance Gene Detection

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