“…Gut samples were collected in tubes containing 0Á2 g l À1 bactopeptone (Becton, Dickinson and Co, Stockholm, Sweden) with 0Á15 g l À1 Tween 80 (Merck KGaA, Darmstadt, Germany) and stored at 4°C until agar plating on the following day. For three individual fish per tank (n = 12 per treatment), the pH of the distal gut content was analysed using an Orion ROSS TM micro-electrode and Orion Star TM pH meter (Thermo Fisher Scientific Inc., Waltham, MA) and values were temperature-corrected from 25°C to adjust to fish in water temperatures of 11 and 18°C according to Inaba et al (1986).…”