New enzyme biosensor for determination of glucose

Gavrilov, A.B.; Zueva, A.F.; Ефимов, О. Н.; Bogdanovskaya, V. A.; Tarasevitch, M.R.

doi:10.1016/0379-6779(93)91236-u

Cited by 12 publications

(4 citation statements)

References 11 publications

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“…A great deal of work on the development of glucose sensors has been carried out by Heller's group (1996). Several techniques such as electrochemical (Gavrilov et al, 1993), Langmuir-Blodgett (Hodak et al, 1997), layer-by-layer (Onda et al, 1996;Kenausis et al, 1997) and covalent binding (Okahata et al, 1989) techniques have been developed for the immobilization of enzymes on various matrices, and electrochemical adsorption of enzymes has been reported for the construction of enzyme electrodes (Sadik and Wallace, 1993;Compagnone et al, 1995). The incorporation of the enzyme into the growing conducting polymer during electropolymerization has recently been used as a convenient and simple method to achieve the immobilization.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films

Liu

Neoh

Chen

et al. 2004

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films

Liu

Neoh

Chen

et al. 2004

Biosensors and Bioelectronics

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“…A product of the reaction of the substrate with oxygen catalysed by these enzymes is H 2 O 2 , and its electroactivity can be used to obtain a measurable current signal. For glucose detection, the most widely used biosensors that have been studied are amperometric enzyme-based electrodes [3,4] with carbon or with noble metal electrode substrates such as Au and Pt [5,6].…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of electrochemical glucose enzyme biosensors based on carbon film electrodes

Florescu

Brett

2005

Talanta

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Electrochemical glucose enzyme biosensors have been prepared on carbon film electrodes made from carbon film electrical resistors. Evaluation and characterisation of these electrodes in phosphate buffer saline solution has been carried out with and without pretreatment by cycling in perchloric acid or at fixed applied potential. Both pretreatments led to a reduction in the carbon surface oxidation peak and enabled better detection of hydrogen peroxide in the pH range of 5-7. Glucose oxidase enzyme was immobilised on the carbon surface by mixing with glutaraldehyde, bovine serum albumin and with and without Nafion. The performance of these two types of electrode was similar, that containing Nafion being more physically robust. Linear ranges were up to around 1.5mM, with detection limits 60muM, and pretreatment of the carbon film electrode at a fixed potential of +0.9V versus SCE for 5min was found to be the most beneficial. Michaelis-Menten constants between 5mM and 10mM were found under the different experimental conditions. Coating the immobilised enzyme layer with a thin layer of Nafion was found to give similar results in the determination of glucose to mixing it but with benefits against interferences for the analysis of complex matrices, such as wine. Potentialities, for a short-term-use or disposable sensors, are indicated.

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“…electrochemical, covalent binding, Langmuir-Blodgett and layer-by-layer (LBL) techniques have been developed for the immobilization of enzymes on various matrices [6][7][8][9]. The great advantage of the LBL technique is the closepacked deposition of the sensing molecule in very thin layers.…”

Section: Several Techniques Such Asmentioning

confidence: 99%

Nano-assembly of glucose oxidase on thein situself-assembled films of polypyrrole and its optical, surface and electrochemical characterizations

Ram¹,

Adami²,

Paddeu³

et al. 2000

Nanotechnology

106

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An in situ self-assembly technique with nanometre control over thicknesses and multilayered structures was used to manufacture the ultrathin films of polypyrrole (PPY). The PPY film was deposited on a polyanion, poly(styrene sulfonate) (PSS) modified surface as a function of time, previously PSS had been deposited on various substrates (glass, mica, indium-tin-oxide coated glass plates). Later, alternate PPY and PSS films were fabricated on such substrates using a layer-by-layer (LBL) technique. The glucose oxidase (GOD) molecules were also deposited on such self-assembled PPY films by the LBL technique. The PSS/PPY/GOD, PPY/PPY/GOD/poly(ethylene imine) (PEI)/GOD etc, film configurations were fabricated, and functionally as well as structurally characterized by UV-visible, electrochemical and scanning probe microscopy techniques, respectively. The results of a scanning probe microscopic study on the films were analysed to understand the molecular orientation of GOD on the PPY surface, and the dependence of the enzyme concentration for the depositing solutions. Moreover, the electrochemical studies performed provided information with respect to the electron transfer processes on the spatial arrangement of GOD molecules on the PPY surfaces. The immobilization of GOD on a conductive PPY represented a crucial and important step, and allowed us to construct the glucose-responsive biosensors.

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New enzyme biosensor for determination of glucose

Cited by 12 publications

References 11 publications

Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films

Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films

Development and evaluation of electrochemical glucose enzyme biosensors based on carbon film electrodes

Nano-assembly of glucose oxidase on thein situself-assembled films of polypyrrole and its optical, surface and electrochemical characterizations

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