New enzymatic assays based on the combination of signal accumulation type of ion sensitive field effect transistor (SA-ISFET) with horseradish peroxidase

Tomari, Naohiro; Sasamoto, Kohei; Sakai, Hanami; Tani, Tadaaki; Yamamoto, Yoshihiro; Nishiya, Yoshiaki

doi:10.1016/j.ab.2019.113353

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Multiple studies have previously demonstrated the ability to sense redox species using FET-based technologies. However, the FET-based sensors in these studies have utilized reactive gate materials, such as iridium oxide, or active redox mediators, such as HRP and Prussian blue [22,24,25]. Evidently, redox species sensing may also be achieved through nanowire FET surface modification of the gate material, by coupling known redox mediators such as quinone/hydroquinone [27].…”

Section: Discussionmentioning

confidence: 99%

“…The E MOSFET and other potentiometric solid state-based sensors use a fixed gate potential setup by gate grounding. Gate materials generally rely on reactive substrates to detect such redox species, including different oxides, e.g., silicon oxide and iridium oxide [20,21], and surface modifiers (e.g., self-assembled monolayers (SAM), quinone, Prussian blue, horseradish proxied (HRP) [22][23][24][25], which require specific chemical conditions to control their oxidation states. The use of noble metal as gate electrode building material, on the other hand, should allow for a relatively fast and reversible electrochemical response.…”

Section: Introductionmentioning

confidence: 99%

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Potentio-tunable FET sensor having a redox-polarizable single electrode for the implementation of a wearable, continuous multi-analyte monitoring device

et al. 2022

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The emerging field of wearable devices for monitoring bioanalytes calls for the miniaturization of biochemical sensors. The only commercially available electrochemical wearable monitoring medical devices for bioanalytes are the amperometric continuous glucose monitoring (CGM) systems. The use of such amperometric methods to monitor glucose levels requires a relatively large electrode surface area for sufficient redox species collection, allowing accurate measurements to be made. Consequently, miniaturization of such sensors bearing large electrodes is challenging. Furthermore, it is difficult to introduce and deploy more than one electrode–based sensor per device, thereby limiting the number of analytes that can be monitored in parallel. To address these limitations, we have employed a non-referenced, single polarizable electrode coupled to a fin-shaped field-effect transistor (Fin-FET). We have discovered that by passivating the FET area by a relatively thick oxide and/or polytetrafluoroethylene (PTFE) polymer, leaving only the polarizable working electrode (WE) exposed, we can monitor redox analytes at the micromolar to millimolar concentration range. We attribute this effect to the WE polarization by the solution redox species. We have exploited the superior sensitivity of the adjacent silicon-based Fin-FET to detect changes in sensor electrode potentials induced by the redox species. Furthermore, we demonstrated the correlation between a specific analyte and the biasing WE potential on the accumulation/depletion of the coupled Fin-FET channel as manifested by the transistor source-drain current. Moreover, we utilized the analyte-electrode potential interaction, which is analyte-specific, to tune the specificity of the sensor towards an analyte of choice. In addition, we demonstrated the use of a single-electrode potentiometric sweep to assist in identifying the accumulation/depletion as a result of analyte-WE state. Collectively, the tiny potentio-tunable electrochemical sensor (PTEchem sensor) area is ~50 × 50 µm, and dedicated wireless transducer facilitates the use of this sensor for wearable continuous, multi-metabolite monitoring. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potentio-tunable FET sensor having a redox-polarizable single electrode for the implementation of a wearable, continuous multi-analyte monitoring device

et al. 2022

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ISFET‐based sensors for (bio)chemical applications: A review

Cao

Sun

Xiao

et al. 2022

Electrochemical Science Adv

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Ion‐sensitive field effect transistor (ISFET) sensor is a hot topic these years, playing the combined roles of signal recognizer and converter for (bio)chemical analytes. In this review article, the basic concept, origination, and history of the ISFET sensor are presented. In addition, the common fabrication processes, the most‐used working principle (potentiometric, amperometric, and impedancemetric), and the techniques of gate functionality (physical, chemical, and biological) are discussed introducing the afterward signal transfer processes from ISFET to the terminals through different types of circuits. At last, the development and recent progress (until 2021) of ions and biomolecules (DNA molecules, antibodies, enzymatic substrates, and cell‐related secretions or metabolism) were introduced together with the outlook and facing obstacles (Debye screening, the wearability of ISFET, the multiplexed detections) before the commercialization of ISFET. This review article emphasizes the advantages of the developed ISFET sensors as miniaturization, low‐cost, all‐solid, highly sensitive, and easy operation for portable and multiplexed detections.

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New enzymatic assays based on the combination of signal accumulation type of ion sensitive field effect transistor (SA-ISFET) with horseradish peroxidase

Cited by 2 publications

References 22 publications

Potentio-tunable FET sensor having a redox-polarizable single electrode for the implementation of a wearable, continuous multi-analyte monitoring device

Potentio-tunable FET sensor having a redox-polarizable single electrode for the implementation of a wearable, continuous multi-analyte monitoring device

ISFET‐based sensors for (bio)chemical applications: A review

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