New Enhancement Spectrofluorimetric Determination of Anti‐Breast Cancer Drugs Palbociclib by Biogenic‐Synthesized Alumina/NiO Nanocomposite

Abstract

This study is based on green synthesized nanomaterials that increase fluorescence intensity in the study of the anticancer drug palbociclib. The basis for the nanomaterials was the use of alumina/nickel oxide nanocomposite (alumina/NiO NCS) biogenically produced from millet seed extract. The proposed materials improved the spectrofluorimetric method for the study of the breast cancer drug palbociclib (PAL) in its real powder and capsules. The amplification properties of the developed spectrofluorimetric system depend on the unique optical properties of the alumina/NiO NCS. Fluorescence detection was determined at λex 260 and at λem 430 nm. The effect of surfactant (sodium dodecyl sulfate, SDS) was investigated, and the results obtained with the PAL alumina/NiO/SDS showed linearity of 1.0–800 g/mL for PAL samples with a correlation coefficient of r>0.999. The aforementioned fluorescence approach showed lower detection and quantification of 0.03 and 1.0 g/mL, respectively. The proposed system was potentially efficient for the determination of PAL with an excellent percent recovery of 99.50±0.5 %. The effect of some co‐formulated interfering substances such as crystalline cellulose, starch, silicon dioxide magnesium stearate, lactose and gelatine that present in the capsules was investigated and no remarkable difference was found during the assay process of PAL. The method was validated to ensure its suitability and showed high sensitivity and accuracy for the detection of PAL.