We determined the complete mitochondrial genome (mitogenome) sequence of a Chilean freshwater crayfish,
Parastacus nicoleti
(Philippi, 1882). This is the first complete mitogenome sequence of a non-Australian crayfish belonging to the family Parastacidae. The complete mitogenome of
P. nicoleti
is 20,894 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a putative control region (CR). In the phylogenetic analysis, freshwater crayfishes were clearly divided into two monophyly groups, Northern and Southern Hemisphere groups. The
P. nicoleti
exhibited a sister-group relationship with all other Australian parastacid crayfishes.