“…The history of vaccines began in 1796, when the pioneer of smallpox vaccine Edward Jenner (1749-1823) demonstrated the protective effect of the cowpox virus against the smallpox virus infection in humans (BAXBY, 1999 [2]). Initially, empirical vaccines (derived from killed pathogenic organism) were used, but the development of biological sciences (e.g., biochemistry, cell culture technology, immunology, molecular biology, molecular genetics) led to a new generation of vaccines, safer and immunogenic, that are based on scientific design approaches (TOMAR & al, 2005 [1]; PLOTKIN, 2014 [3]). In the same way, the vaccine potency assays evolved from the expensive and laborious in vivo assays (e.g., immunization of dozens of animals) to the inexpensive and simple modern analytical tools (e.g., immunochemical methods) (VERCH & al, 2018 [4]).…”