New dimensions in tumor immunology: what does 3D culture reveal?

Feder-Mengus, Chantal; Ghosh, Sourabh; Reschner, Anca; Martin, Ivan; Spagnoli, Giulio C.

doi:10.1016/j.molmed.2008.06.001

Cited by 124 publications

(97 citation statements)

References 81 publications

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“…As cancer spheroids are more likely to mirror the 3D cellular context and therapeutically relevant pathophysiological gradients of in vivo tumors, 20,21 we further tested if human MC could also promote the growth of colon cancer spheroids. To answer this, we developed a coculture model of HT29 spheroids and human MC embedded in an extracellular matrix (ECM).…”

Section: Resultsmentioning

confidence: 99%

Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models

Blokhuis

Derks

et al. 2018

OncoImmunology

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Chronic inflammation drives the development of colorectal cancer (CRC), where tumor-infiltrating immune cells interact with cancer cells in a dynamic crosstalk. Mast cells (MC), one of earliest recruited immune cells, accumulate in CRC tissues and their density is correlated with cancer progression. However, the exact contribution of MC in CRC and their interaction with colon cancer cells is poorly understood. Here, we investigated the impact of primary human MC and their mediators on colon cancer growth using 2D and 3D coculture models. Primary human MC were generated from peripheral CD34+ stem cells. Transwell chambers were used to analyze MC chemotaxis to colon cancer. Colon cancer cells HT29 and Caco2 differentially recruited MC by releasing CCL15 or SCF, respectively. Using BrdU proliferation assays, we demonstrated that MC can directly support colon cancer proliferation and this effect was mediated by their cellular crosstalk. 3D coculture models with cancer spheroids further confirmed the pro-tumor effect of MC on colon cancer growth, where direct cell-cell contact is dispensable and increased production of multiple soluble mediators was detected. Moreover, TLR2 stimulation of MC promoted stronger growth of colon cancer spheroids. By examining the transcriptome profile of colon cancer-cocultured MC versus control MC, we identified several MC marker genes, which were deregulated in expression. Our study provides an advanced in vitro model to investigate the role of human MC in cancer. Our data support the detrimental role of MC in CRC development and provide a molecular insight into the cellular crosstalk between MC and colon cancer cells.

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Section: Resultsmentioning

confidence: 99%

Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models

Blokhuis

Derks

et al. 2018

OncoImmunology

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“…They showed that the 3D culture better recapitulates different tumors' behavior than did 2D configurations. For example, it was demonstrated that the 3D context was able to reverse tumor phenotype to normal (with specific pathways' inhibitors), and to sustain the cross-talk between integrin and EGF receptors; moreover, tumor cells cultured in 3D showed, with respect to 2D-cultured controls, an increased pro-angiogenic capacity and a higher resistance to IFN, chemotherapeutic agents and irradiation [82,83]. Other parameters that are highly modified by the switch from 2D to 3D configuration are the cell migration, cell morphology and cell signaling [84,85].…”

Section: D Versus 3d Cancer Modelsmentioning

confidence: 99%

“…Cells grown in MSs usually exhibit a lower sensitivity to therapeutic drugs and a more specific response to particular therapeutic agents as a result of new signaling pathways activation [94,95]. In living organisms, in addition to the involvement of particular proteins (such as the drug/ABC transporters, enzymes and HMGB1), or to the increased efficiency in DNA repairing mechanisms and resistance to apoptosis, multi-drug resistance may also be due to ECM's organization and composition (desmoplasia), cell-cell adhesion and lower growth rate, which are parameters that mirror the in vivo context, in which tissue architecture and cell adhesion, as well as the absence of blood vessel and the low hydrostatic and osmotic pressure, limit the diffusion of therapeutic drugs [83,96,97] .…”

Section: D Tumor Modelsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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“…3D cultures employ different methods which include liquid-overlay technique, spinner flasks, roller bottles etc. [5] . Another culture technique that involves the co-culture of different cell types is necessary for studying cellular interactions and has a wide range of other applications from feeder cell provisions to provide specific growth factors such as cytokines, to the study of bystander effects for studies such as radiobiology.…”

Section: Solid Tumor Cell Lines Culture Modelsmentioning

confidence: 99%

Discovery tools for solid tumor research

Ravi

Balaji

Haridoss

2012

JST

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Solid tumors take center stage as a primary human health concern as they comprise the majority of cancer incidences and mortalities. It is heartening however, to note the decreasing incidence rates as well as better survival rates over the past decade, largely owing to preventive measures, healthy life styles and developments in diagnostic, therapeutic and management areas which in turn are due to the rapid progress in cancer research. Many tools are available for cancer research of which, cell lines obtained from either the solid tumor site or from metastatic sites, are pivotal. More than 700 cell lines have been established from solid tumors ranging from the carcinomas and lymphomas to the sarcomas. Over the past decade, developments in cell culture techniques viz. the three dimensional culture systems and xenotransplants, have increased the potential application of such cell lines. Apart from the cell lines and various culture techniques, other material such as tumor tissue biopsies, tissue lysates and tissue arrays from cancerous, adjacent and normal tissue are major contributors to cancer research. While each one has its own advantages and limitations, a combined approach gives us an over-all picture of solid tumor research development over the recent past and the future directions available.

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New dimensions in tumor immunology: what does 3D culture reveal?

Cited by 124 publications

References 81 publications

Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models

Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Discovery tools for solid tumor research

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