New device for the measurement of hydrogen clearance rate from the gastric mucosa

Abstract

The hydrogen clearance rate of the nucosa of the glandular stomach of the anesthetized rat was obtained with the platinum electrode in contact with the surface of the gastric mucosa (Contact Electrode Method). By using two indicator electrodes, the gastric blood flow was measured simultaneously with one electrode in contact with the mucosal surface (Contact Electrode Method), and with the other electrode inserted into the gastric wall (Inserted Electrode Method). Pentagastrin produced increase in blood flow by both contact and inserted electrode methods. Isoproterenol stimulated blood flow as determined by the contact electrode method, but when the flow was determined by the inserted electrode method it reduced the blood flow. The combination of these two methods is useful in the determination of the blood flow in the different layers of the stomach.

Effects of Omeprazole on Gastric Mucosal Blood Flow in the Conscious Rat

“…The resting blood flow in the corpus mucosa was slightly higher than that in the antral mucosa. This is in agreement with previous findings in conscious animals (25,26), whereas in anaesthetized animals it is generally reported that antral blood flow is higher than the corpus blood flow (21)(22)(23)(24).…”

“…The basal blood flow values in the stomachs of the conscious rats were higher than those reported in anaesthetized animals (21)(22)(23)(24). The resting blood flow in the corpus mucosa was slightly higher than that in the antral mucosa.…”

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“…Using the well-validated hydrogen gas clearance technique in more recent studies, increases in mucosal blood flow were also observed in the rat during stimulation of acid secretion by pentagastrin infusion (Murakami et al, 1982;Leung et al, 1984;Pique et al, 1992a). Furthermore, using both direct visualization of rat submucosal vessels and laser Doppler flowmetry, pentagastrinstimulated acid secretion was associated with an increase in microvascular perfusion, although a direct correlation between these parameters was not observed (Holm-Rutili & Berglindh, 1986).…”