New Developments in Wound Healing Relevant to Facial Plastic Surgery

Hom, David B.

doi:10.1001/archfaci.10.6.402

Cited by 26 publications

(16 citation statements)

References 29 publications

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“…Typically, the concentration of platelets in PRP is about three to six times the normal platelet concentration in peripheral blood [27,28], and can therefore generate an elevated concentration of growth factors [11,27,29]. This supraphysiological factor concentration in PRP is achieved through platelet activation, degranulation and release of their stored growth factors [11,[29][30][31], e.g., VEGF (vascular endothelial growth factor), PDGF (platelet-derived growth factor), EGF (epidermal growth factor), IGF-1 (insulin-like growth factor-1), bFGF (basic fibroblast growth factor), TGF-beta1 (transforming growth factor beta-1), TSP-1 (thrombospondin-1) and PF-4 (platelet factor-4) [23,[31][32][33][34][35]. Evidently, PRP is a composite mixture of both angiogenesis-promoting (e.g., VEGF, PDGF) and angiogenesis-inhibiting (e.g., TSP-1, PF-4) protein factors, which makes the analysis of this secretome's net angiogenic effect(s) inherently complex.…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of the Angiogenic Potential of Hypoxia Preconditioned Blood-Derived Secretomes and Platelet-Rich Plasma: An In Vitro Analysis

et al. 2020

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Blood-derived factor preparations are being clinically employed as tools for promoting tissue repair and regeneration. Here we set out to characterize the in vitro angiogenic potential of two types of frequently used autologous blood-derived secretomes: platelet-rich plasma (PRP) and hypoxia preconditioned plasma (HPP)/serum (HPS). The concentration of key pro-angiogenic (VEGF) and anti-angiogenic (TSP-1, PF-4) protein factors in these secretomes was analyzed via ELISA, while their ability to induce microvessel formation and sprouting was examined in endothelial cell and aortic ring cultures, respectively. We found higher concentrations of VEGF in PRP and HPP/HPS compared to normal plasma and serum. This correlated with improved induction of microvessel formation by PRP and HPP/HPS. HPP had a significantly lower TSP-1 and PF-4 concentration than PRP and HPS. PRP and HPP/HPS appeared to induce similar levels of microvessel sprouting; however, the length of these sprouts was greater in HPP/HPS than in PRP cultures. A bell-shaped angiogenic response profile was observed with increasing HPP/HPS dilutions, with peak values significantly exceeding the PRP response. Our findings demonstrate that optimization of peripheral blood cell-derived angiogenic factor signalling through hypoxic preconditioning offers an improved alternative to simple platelet concentration and release of growth factors pre-stored in platelets.

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Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of the Angiogenic Potential of Hypoxia Preconditioned Blood-Derived Secretomes and Platelet-Rich Plasma: An In Vitro Analysis

et al. 2020

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“…They promote cell division and proliferation, increase collagen synthesis, stimulate angiogenesis, induce cell differentiation and remove necrotic tissue, to accelerate the wound repair and tissue regeneration. In addition, platelets release coagulation factors, serotonin histamine, endostatin and hydrolytic enzymes 4 .…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich fibrin in plastic surgery

Yj¹,

Si²

2013

OA Evidence-Based Medicine

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Introduction Platelet concentrate has been used in surgery for many years. The initial concept of these autologous preparations was to concentrate platelets and their growth factors and to deliver it to a surgical site, in order to improve local healing. Platelet-rich fibrin (PRF) is the new generation of platelet concentrate. Choukroun's PRF (L-PRF) is the latest development of platelet concentrate protocols, which was first developed in France by Choukroun et al. in 2001 as an autologous biomaterial. PRF is obtained centrifugally by autologous peripheral blood, without adding any biological agents. PRF contains the fibrin matrix polymer, leucocytes, cytokines and circulating stem cells. PRF could be classified into two categories, depending on their leucocyte content: pure plateletrich fibrin (P-PRF) and leucocyteand PRF (L-PRF). PRF is produced with a simple method, it is low cost and easily available, which has been applied in many different fields, particularly oral and maxillofacial, orthopaedic and plastic surgery. L-PRF and PRFM (P-PRF) are both applied in plastic surgery and the applications can be divided into two aspects: facial plastic surgery and wound healing. The aim of this review was to discuss platelet-rich fibrin in plastic surgery. Conclusion Unfortunately, this field of research suffered from some problems for many years, such as the lack of a coincident terminology and the leucocyte content. Further studies are necessary to validate the interest of the PRF in plastic surgery. This review describes the classification, preparation, applications and problems of PRF in plastic surgery.

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“…The approval of growth factor products by the U.S. Food and Drug Administration (FDA) heralded a new era for approaches that "jump-start" healing of surgical wounds. 4 Over the past decade, there has been rapid proliferation of biological and clinical tools to promote cellular replication and healing. abrasion, a burn, a contusion, or a laceration, an immediate vascular and inflammatory response occurs.…”

Section: History To Present Daymentioning

confidence: 99%

“…30 Fibroblasts migrate and replicate in response to mediators (C5a, fibronectin, PDGF, FGF-2, and TGF-b). 4 Fibroblasts are also important in the production of elastin, fibronectin, glycosaminoglycans, and collagenase, which is important in the later maturation and remodeling phase. 31 Fibroblasts, along with perivascular mesenchymal cells, differentiate into myofibroblasts, which allow for wound contraction.…”

Section: Proliferative Phasementioning

confidence: 99%