New developments in the detection and identification of processed animal proteins in feeds

The aim of the study was to gather and analyse available data concerning the presence of terrestrial animal constituents in feeds in Poland. A microscopic method of identification of the contaminants was used. Between 2012 and 2013, overall 16 139 samples of feeds were analysed, from which 282 (1.75%) contained elements from terrestrial animals. The percentage of feeds contaminated with such elements was lower in 2013. In 2012, among 8 499 samples analysed, 203 (2.39%) contained ingredients of animal origin, and in 2013, the elements were found in 79 (1.03%) out of 7640 samples. The percentage of feed samples positive for processed animal protein was relatively low and steadily decreasing. Furthermore, the microscopic method demonstrated to be a very sensitive technique for the detection of constituents of animal origin.

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“…This method is very sensitive, because it allows the detection of animal-origin elements at the requested level of 1 g/kg (2,20,21,25,28).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Animal-Origin Constituents in Feeds

Weiner

Paprocka

Golebiowska

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Μια λεπτομερής ανασκόπηση στο αντικείμενο αυτό έχει δημοσιευθεί από τους van Raamsdonk et al (2007). Οι ερευνητές αυτοί παρουσιάζουν τα απο τελέσματα 5 διαφορετικών Κοινοτικών μελετών επάρ κειας εργαστηρίων (ring-tests) που οργανώθηκαν από το 2003 και εντεύθεν.…”

Section: άλλες μέθοδοιunclassified

Feed Ban and BSE: the detection and identification of processed animal proteins in compound feeds

Zoiopoulos¹

2017

J Hellenic Vet Med Soc

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Reference is made initially to the incidence of BSE within the EU territory and the reasons of the appearance of the disease i.e. the use of meat and bone meal in ruminant diets. In turn, the evolution of Community legislation in the sector of marketing of feeds of animal origin is described. Furthermore, the banning of feeding of mammalian protein to ruminants and the extension of prohibition to further animal proteins such as from other vertebrates and how this banning affects the feeding of the various animal species is reviewed. Finally, the importance of detecting and identifying the presence and amount of processed animal proteins in compound feed as well as feed materials is stressed and methods such as feed microscopy, immunoassay techniques, PCR, NIR and HPLC are discussed.

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“…Light microscopy in combination with computer image analysis (IA), which is based on the identification of bone particles or tissues in feedingstuffs, has been also proposed (Ottoboni et al, 2014;Pinotti et. al., 2013;van Raamsdonk et al, 2007). Findings in these studies have indicated that the use of the microscopic method in association with computer image analysis to identify the origin of PAPs appears promising, especially as a complementary method to the DNA-based ones.…”

Section: Introductionmentioning

confidence: 98%

Microscopy in combination with image analysis for characterization of fishmeal material in aquafeed

Pinotti

Ottoboni

Caprarulo

et al. 2016

Animal Feed Science and Technology

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The aim of this study was to investigate the use of microscopy in combination with image analysis (IA) measurements for the characterization of fish bone lacunae in aquafeedextracted material. For this purpose two experiments have been conducted. In experiment (exp.) 1, six samples of fish meal based aquafeed were analyzed by the microscopic method, according to Annex VI of Regulation 152/2009. Sediment fractions of each sample were observed with a compound microscope at X40. Two hundred and fifty eight bone fragment lacunae images were recorded and processed through IA software. Accordingly, on each lacuna 30 geometric variables have been obtained and measured. The geometric variables have been grouped in two main families, namely size descriptors and derived shape descriptors. In exp. 2 measurements obtained from 1081 bone lacunae (644 for mammals and 437 for poultry) acquired from 14 mammalian and 7 poultry samples have been merged with the aquafeed dataset (258 bone lacunae). Results obtained in exp. 1 indicated that nearly two thirds of the descriptors presented differences among the analyzed samples. Differences in observed values were not systematically distributed among the six samples. Nevertheless, in all analyzed samples features of lacunae have shown an overlap. By contrast the comparison of fish bone lacunae with avian and mammalian bone lacunae (exp. 2), has revealed a large gap between terrestrial and aquatic animals in the case of several descriptors (16 primary and 8 secondary descriptors). Therefore, it can be concluded that combining light microscopy and image analysis: (i) fish material in aquafeed appears quite homogenous in term of bone features; ii) fish material can be distinguished from avian and mammalian materials by selecting specific descriptors.

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New developments in the detection and identification of processed animal proteins in feeds

Cited by 66 publications

References 28 publications

Occurrence of Animal-Origin Constituents in Feeds

Occurrence of Animal-Origin Constituents in Feeds

Feed Ban and BSE: the detection and identification of processed animal proteins in compound feeds

Microscopy in combination with image analysis for characterization of fishmeal material in aquafeed

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