New detection system for toxic agents based on continuous spectroscopic monitoring of living cells

Notingher, Ioan; Selvakumaran, Jamuna; Hench, Larry L.

doi:10.1016/j.bios.2004.04.008

Cited by 81 publications

(88 citation statements)

References 20 publications

(39 reference statements)

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“…As shown here, both cell types are highly responsive to a wide range of toxins including those that induce membrane damage, DNA damage, and inhibition of metabolic activity. We have found that membrane damage is readily quantified using a fiber-delivered infrared spectroscopic measurement [32][33], whereas internal damage may best be quantified through Raman spectroscopic measurements [34]. Macrophages appear to be more sensitive to many of these biotoxins; however, their low adherence to surfaces and high variability in response makes their use in a biosensor problematic and requires further evaluation.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Bacterial-Derived Toxins to Immortal Lung Epithelial and Macrophage Cells

Peterson

Collier

Katterman

et al. 2009

Appl Biochem Biotechnol

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Health risks associated with inhalation and deposition of biological materials have been a topic of great concern due to highly publicized cases of inhalation anthrax, of new regulations on the release of particulate matter, and to increased concerns on the hazards of indoor air pollution. Here, we present an evaluation of the sensitivity of two immortal cell lines (A549, human lung carcinoma epithelia) and NR8383 (rat alveolar macrophages) to a variety of bacterial-derived inhalation hazards and simulants including etoposide, gliotoxin, streptolysin O, and warfarin. The cell response is evaluated through quantification of changes in mitochondrial succinate dehydrogenase activity, release of lactate dehydrogenase, initiation of apoptosis, and through changes in morphology as determined by visible light microscopy and scanning electron microscopy. These cells display dose-response relations to each toxin, except for triton which has a step change response. The first observable responses of the epithelial cells to these compounds are changes in metabolism for one toxin (warfarin) and alterations in membrane permeability for another (gliotoxin). The other four toxins display a similar time course in response as gauged by changes in metabolism and loss of membrane integrity. Macrophages are more sensitive to most toxins; however, they display a lower level of stability. This information can be used in the design of cell-based sensors responding to these and similar hazards.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Bacterial-Derived Toxins to Immortal Lung Epithelial and Macrophage Cells

Peterson

Collier

Katterman

et al. 2009

Appl Biochem Biotechnol

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“…At present Fourier Transform Infrared Microspectrosopy (FTIRM) and Confocal Raman Microspectroscopy (CRM) have identified in-situ molecular alterations associated with cell death via apoptosis [1][2][3] or necrosis [4,5], or as a result of proliferative changes in cellular activity [6][7][8] or mitosis [9]. It has also been shown that these modalities can identify molecular changes associated with changes in the phenotype of differentiating embryonic cells [10,11].…”

Section: Introductionmentioning

confidence: 99%

Growth substrate induced functional changes elucidated by FTIR and Raman spectroscopy in in–vitro cultured human keratinocytes

Meade¹,

Lyng²,

Knief³

et al. 2006

Anal Bioanal Chem

109

115

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“…These peaks are only a few examples of features of the detailed spectra and a complete assignment can be easily found in the literature 13,14,[32][33][34][35] .…”

Section: Collagen Gels As Suitable Substrates For Raman Spectroscopy mentioning

confidence: 99%

Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

Bonnier¹,

Meade²,

Merzha³

et al. 2010

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Three dimensional collagen gels are evaluated as matrices for the study of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. It is demonstrated, using the Alamar Blue assay, that both cell models exhibit enhanced viability in collagen matrices compared to quartz substrates, commonly used for Raman spectroscopy. Using principal component analysis, it is shown that the Raman spectral analysis of cells in collagen matrices are minimally contaminated by substrate contributions and the cell to cell spectral variations are greatly reduced compared to those measured on quartz substrates. Furthermore, the spectral measurements are seen to have little contribution from the cell culture medium, implying that cultures can be kept viable over prolonged measurement or mapping procedures.

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New detection system for toxic agents based on continuous spectroscopic monitoring of living cells

Cited by 81 publications

References 20 publications

Cytotoxicity of Bacterial-Derived Toxins to Immortal Lung Epithelial and Macrophage Cells

Cytotoxicity of Bacterial-Derived Toxins to Immortal Lung Epithelial and Macrophage Cells

Growth substrate induced functional changes elucidated by FTIR and Raman spectroscopy in in–vitro cultured human keratinocytes

Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

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