New Delhi Metallo-Beta-Lactamase-Producing Enterobacteriaceae in South Korea Between 2010 and 2015

Yoon, Eunju; Kang, D. Y.; Yang, Jiyeon; Kim, Dokyun; Lee, Hyukmin; Lee, Kwang Jun; Jeong, Seok Hoon

doi:10.3389/fmicb.2018.00571

Cited by 44 publications

(44 citation statements)

References 24 publications

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“…Their great intracellular versatility and rapid evolution of their replicons’ regulatory sequences allow them to succeed in spreading among Enterobacteriaceae . This has been shown in most studies focusing on KPC‐ and NDM‐producing E. coli and K. pneumoniae in different countries …”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 95%

“…Larger plasmids, such as pPMK1‐NDM (304.5 kb) and pNDM‐EcoGN568 (166.7 kb), are examples of NDM‐1–containing plasmids that contain other resistance determinants, including β‐lactamases, with pPMK1‐NDM containing a large conjugative transfer module . Other IncF plasmids, including pEh1A, pNDM‐Ec1GN574, pKOX‐NDM‐1, and pCRCB‐101_1, are also responsible for the dissemination of bla NDM‐1 in other species, such as C. freundii , Enterobacter hormaechei , and Klebsiella michiganensis . pNDM‐Ec1GN574 and pKOX‐NDM1 plasmids are similar in size (110.8 kb), with the NDM region being flanked by 256 bp direct repeats that are suggested to be responsible for the acquisition of bla NDM‐1 .…”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

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Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities. Resistance to multiple β‐lactams complicates effective clinical management of CRE infections. Using plasmid typing methods, a wide distribution of plasmid replicon groups has been reported in CREs around the world, including IncF, N, X, A/C, L/M, R, P, H, I, and W. We performed a literature search for English research papers, published between 2013 and 2018, reporting on plasmid‐mediated carbapenem resistance. A rise in both carbapenemase types and associated plasmid replicon groups was seen, with China, Canada, and the United States recording a higher increase than other countries. blaKPC was the most prevalent, except in Angola and the Czech Republic, where OXA‐181 (n = 50, 88%) and OXA‐48–like (n = 24, 44%) carbapenemases were most prevalent, respectively; blaKPC‐2/3 accounted for 70% (n = 956) of all reported carbapenemases. IncF plasmids were found to be responsible for disseminating different antibiotic resistance genes worldwide, accounting for almost 40% (n = 254) of plasmid‐borne carbapenemases. blaCTX‐M, blaTEM, blaSHV, blaOXA‐1/9, qnr, and aac‐(6′)‐lb were mostly detected concurrently with carbapenemases. Most reported plasmids were conjugative but not present in multiple countries or species, suggesting limited interspecies and interboundary transmission of a common plasmid. A major limitation to effective characterization of plasmid evolution was the use of PCR‐based instead of whole‐plasmid sequencing–based plasmid typing.

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Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 95%

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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“…The predominant strain of this outbreak, E coli ST361, is not internationally well‐known as an NDM producer. According to Yoon et al, the distribution of NDM‐5 producers in South Korea from 2010 to 2015 shows that E coli is the largest (14/18, 77.8%) and among them, ST 101 accounted for 77.8% (9/14), followed by ST362, ST361, ST162, ST90, and ST88 each accounted for 7.1% (1/14). The E coli ST361 harboring bla NDM‐5 only accounted for 7.1% (1/14) and did not belong to the most common clones, such as ST101, in South Korea.…”

Section: Discussionmentioning

confidence: 99%

“…Clusters were defined as DNA patterns sharing >85% similarity. PCR and sequencing for MLST were carried out for seven housekeeping genes per species: adk , fumC , gyrB , icd , mdh , purA , and recA for E coli and the sequences were compared in the MLST database, so that allelic numbers and sequence types (STs) could be determined . The allelic profiles and STs were assigned using an online database (http://mlst.warwick.ac.uk/mlst/dbs/Ecoli).…”

Section: Methodsmentioning

confidence: 99%

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Emergence and transmission of New Delhi metallo‐beta‐lactamase‐5‐producing Escherichia coli Sequence Type 361 in a Tertiary Hospital in South Korea

Park

Choi

Kwon

et al. 2019

Clinical Laboratory Analysis

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Background The emergence of carbapenem‐resistant Escherichia coli (E coli) is a serious global health threat, but little is known about carbapenemase‐producing E coli in Daejeon, South Korea. The aim of this study was to investigate characteristics of thirteen carbapenem‐resistant E coli isolates in a tertiary hospital. Methods Thirteen non‐duplicate carbapenem‐resistant E coli strains were collected from October 2017 to January 2018. Antimicrobial susceptibility was determined with the E test or disk diffusion method. The carbapenem minimum inhibitory concentrations (MICs) were determined by the agar dilution method. The colistin and tigecycline MICs were determined by broth microdilution. The resistance genes, including carbapenemase genes, were evaluated by polymerase chain reaction, and DNA sequencing was performed to characterize the genes. Pulsed‐field gel electrophoresis and multilocus sequence typing (MLST) were performed to evaluate the clonal relatedness of isolates. The clinical data of patients were retrospectively reviewed. Results All the E coli isolates harbored blaNDM‐5 gene and were resistant to most of the antimicrobial agents, such as carbapenem, cephalosporins, ciprofloxacin, and chloramphenicol, excluding amikacin and colistin. Other resistant genes, such as blaTEM‐1, blaCTX‐M‐15, blaCMY‐2, aac(6')‐Ib‐cr, and qepA, were detected. The E coli isolates harboring blaNDM‐5 belonged to ST361 (n = 11), ST12 (n = 1), ST410 (n = 1), and PFGE types A (n = 11), B (n = 1), and C (n = 1). Conclusions This study reports on an outbreak of a predominant epidemic clone, the NDM‐5 producing, multidrug‐resistant E coli ST361 isolate. These findings suggest that we should pay attention to infection control measures to limit the spread of NDM‐5‐producing pathogens.

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