New data on eggshell structure of capillariid species: a SEM perspective

Borba, Victor Hugo; Enoki, Makoto; Lopes-Torres, Eduardo José; Machado-Silva, José Roberto; Iñiguez, Alena Mayo

doi:10.1007/s00436-020-07032-4

Cited by 10 publications

(13 citation statements)

References 14 publications

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“…Calodium hepaticum ), which is a rodent species that is occasionally recorded in carnivores. However, as these eggs were found in field-collected faeces, the origin of parasitism should remain suggestive due to the large number of Capillariidae species infecting wildlife (e.g., birds, mammals, and fish) which can be preyed by wildcats [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

Endoparasites of European Wildcats (Felis silvestris) in Greece

et al. 2021

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The European wildcat (Felis silvestris) is the only wild felid living in Greece. Wildcat populations are declining due to anthropogenic and phenological unfavourable conditions, and parasites may have an additional negative impact. In the present study, the occurrence of endoparasites in wildcats in Greece and the potential threats posed to wildcats, domestic animals, and humans in the study areas has been investigated. In a six-year period, 23 road-killed wildcats and 62 wildcat faecal samples were collected from different areas of the country. Necropsy for the detection of endoparasites and standard parasitological examinations of faecal samples were performed. Parasites were morphologically identified and, in selected cases, molecularly analysed. All necropsied wildcats (100%) were infected by three to 10 different parasite taxa, with the most prevalent being Taenia taeniaeformis (73.9%), Toxocara cati (60.9%), Angiostrongylus chabaudi (56.5%), Ancylostoma tubaeforme (39.1%), Cylicospirura spp. (34.8%), Troglostrongylus brevior (34.8%), and Capillaria aerophila (33.8%). Of the 62 faecal samples examined, 53 (85.5%) were positive for one or more parasite elements (larvae, eggs, or oocysts). The most frequent were T. cati (45.2%), A. chabaudi (29%), C. aerophila (24.2%), and Ancylostomatidae (17.7%). This is the first survey on endoparasites affecting wildcats in Greece. Some of the parasites here found are frequent in domestic and wild felids, while others, i.e., Oslerus rostratus and Cylicospirura petrowi, were described for the first time in the European wildcat. Most of them have a significant pathogenic potential, causing severe to hazardous diseases to infected felids and some, under specific circumstances, can also threaten human health.

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Section: Discussionmentioning

confidence: 99%

Endoparasites of European Wildcats (Felis silvestris) in Greece

et al. 2021

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“…Several reports on Capillaridae eggs detected distinct morphotypes, for example, in Trichuris sp. (Petrželková et al , 2010; Klaus et al , 2017; Garcia-Sánchez et al ., 2020; Kamani et al , 2021), or species of the genera Aonchotheca , Baruscapillaria, Capillaria, Calodium , Echinocoleus , Eucoleus , Pearsonema and Tridentocapillaria (Borba, 2019; Borba et al , 2021a). Interestingly, C. hepaticum eggs from Meriones persicus and R. rattus are described as having a ‘very peculiar morphology’, that is, a punctuated and a radial eggshell ornamentation, with a thick eggshell (5.54 μm) (Borba et al ., 2021b).…”

Section: Discussionmentioning

confidence: 99%

Variability ofCalodium hepaticumeggs from sigmodontine host species through geometric morphometric analysis

et al. 2022

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Calodium hepaticum is a zoonotic nematode with a worldwide distribution. Although the host range of C. hepaticum includes a wide spectrum of mammals (including humans), this parasite is predominantly associated with the families Muridae and Cricetidae. Several Sigmodontinae species from Argentina were found to be infected by C. hepaticum, with a high prevalence in Akodon azarae. The present study focuses on C. hepaticum eggs from natural infection of three species of sigmodontine rodents from Argentina. Eggs were genetically characterized (intergenic 18S rRNA region). The objectives of this work are: (i) to propose a new analytical methodology; and (ii) to morphologically characterize C. hepaticum eggs, from three Sigmodontinae species (A. azarae, Calomys callidus and Oligoryzomys flavescens). Analyses were made by the Computer Image Analysis System based on the new standardized measurements and geometric morphometric tools. The resulting factor maps clearly illustrate global size differences in the parasite eggs from the three Sigmodontinae species analysed. The degree of similarity between egg populations was assessed through pairwise Mahalanobis distances, showing that the largest distances were detected between parasite eggs from C. callidus and O. flavescens. Herein, the phenotypical plasticity of C. hepaticum eggs is shown. Significant positive correlations were obtained between each egg parasite principal component 1 and rodent corporal characteristics: weight; liver weight; rodent length; and rodent body condition. The usefulness of the geometric morphometric analysis in studies of the relationship between C. hepaticum and its host must be highlighted. The high prevalence observed in A. azarae, associated with the wide size range of the parasite eggs evidenced by principal component analysis, suggests A. azarae to be the Sigmodontinae host species that plays the most important role as reservoir host for C. hepaticum in the New World.

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“…3, 4 of Grigonis and Solomon (1976) [43], fig. 1B of [117]) have indicated that the actual surface of an undisturbed C. hepaticum egg is the Pellicula Ovi (vitelline membrane) and that it completely covers most "pores" of the type discussed in the Borba et al (2021) [19] paper. A very clear example of patchiness in the Pellicula Ovi apparently resulting from incidental removal by vortexing is shown in fig.…”

Section: Features Of the Outer Surface Of Chitinmentioning

confidence: 98%

“…Examples of this can be found in the SEM imagery and conclusions of fig. 3 [19] did not adequately take into account that the eggs in these studies had been sonicated and subsequently subjected to other handling and chemical treatments that had caused the Pellicula Ovi to be scrubbed off randomly in some areas of some eggs more so than in others. Note in the SEMs of Eucoleus boehmi [105] in Magi et al (2012) [65] that the delicate Pellicula Ovi had been almost entirely scrubbed off from the eggs in all images, except for a small section apparently remaining over the lower-right quadrant of the Polar Plug in their fig.…”

Section: Features Of the Outer Surface Of Chitinmentioning

confidence: 99%

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Nematode eggshells: A new anatomical and terminological framework, with a critical review of relevant literature and suggested guidelines for the interpretation and reporting of eggshell imagery

Bond

Huffman

2023

Parasite

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A literature review for a recent ultrastructural study of a trichinelloid eggshell revealed consistently occurring errors in the literature on nematode eggshell anatomy. Examples included nematodes of medical, veterinary, and agricultural importance in several orders. Previous researchers had warned of some of these errors decades ago, but a comprehensive solution was not offered until 2012 when a clarifying new anatomical and developmental interpretation of nematode eggshells was proposed by members of the Caenorhabditis elegans Research Community. However, their findings were explained using arcane acronyms and technical jargon intended for an audience of experimental molecular geneticists, and so their papers have rarely been cited outside the C. elegans community. Herein we (1) provide a critical review of nematode eggshell literature in which we correct errors and relabel imagery in important historical reports; (2) describe common reporting errors and their causes using language familiar to researchers having a basic understanding of microscopy and nematode eggs; (3) recommend a new hexalaminar anatomical and terminological framework for nematode eggshells based on the 2012 C. elegans framework; and (4) recommend new unambiguous terms appropriate for the embryonated/larvated eggs regularly encountered by practicing nematodologists to replace ambiguous or ontogenetically restricted terms in the 2012 C. elegans framework. We also (5) propose a resolution to conflicting claims made by the C. elegans team versus classical literature regarding Layer #3, (6) extend the C. elegans hexalaminar framework to include the polar plugs of trichinelloids, and (7) report new findings regarding trichinelloid eggshell structure.

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New data on eggshell structure of capillariid species: a SEM perspective

Cited by 10 publications

References 14 publications

Endoparasites of European Wildcats (Felis silvestris) in Greece

Endoparasites of European Wildcats (Felis silvestris) in Greece

Variability ofCalodium hepaticumeggs from sigmodontine host species through geometric morphometric analysis

Nematode eggshells: A new anatomical and terminological framework, with a critical review of relevant literature and suggested guidelines for the interpretation and reporting of eggshell imagery

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