New cytochrome b haplotypes, harboring L299F or N256S + L299F substitutions, were found in azoxystrobin-resistant Puccinia horiana, the causal agent of chrysanthemum white rust

Matsuzaki, Yuichi; Harada, Toshiyuki; Iwahashi, Fukumatsu

doi:10.1007/s10658-021-02299-4

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…At present, the research on chrysanthemum white rust is mostly focused on physical therapy and chemical therapy (Dheepa et al, 2016;Torres et al, 2017). Although biological fungicides and chemical fungicides can control the occurrence of CWR, P. horiana has developed resistance to various fungicides through gene mutation encoding the target protein of fungicide (Matsuzaki et al, 2021;Nuryani et al, 2021;Gavrilescu and Chisti, 2005). At the same time, excessive use of fungicides will cause serious environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Physiological response of CmWRKY15-1 to chrysanthemum white rust based on TRV-VIGS

Chen

Kuang²,

Wu³

et al. 2023

Front. Plant Sci.

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Chrysanthemum White Rust (CWR) caused by Puccinia horiana Henn. is a major disease in the production process of chrysanthemum, which is widely spread all over the world and can be called “cancer” of chrysanthemum. To clarify the disease resistance function of disease resistance genes can provide a theoretical basis for the utilization and genetic improvement of chrysanthemum resistant varieties. In this study, the resistant cultivar ‘China Red’ was used as the experimental material. We constructed the silencing vector pTRV2-CmWRKY15-1 and obtained the silenced line named TRV-CmWRKY15-1. The results of enzyme activity after inoculation with pathogenic fungi showed that the activities of antioxidant enzymes SOD, POD, CAT and defense-related enzymes PAL and CHI in leaves were stimulated under the stress of P. horiana. In the WT, the activities of SOD, POD and CAT at the peak value were 1.99 times, 2.84 times and 1.39 times higher than that in TRV-CmWRKY15-1, respectively. And the activities of PALand CHI at the peak were 1.63 times and 1.12 times of TRV-CmWRKY15-1. The content of MDA and soluble sugar also confirmed that chrysanthemum was more susceptible to pathogenic fungi when CmWRKY15-1 was silenced. The expression levels of POD, SOD, PAL and CHI at different time points showed that the expressions of defense enzyme related genes were inhibited in TRV-WRKY15-1 under the infection of P. horiana, which weakened the ability of chrysanthemum to resist white rust. In conclusion, CmWRKY15-1 may increased the resistance of chrysanthemum to white rust by increasing the activity of protective enzyme system, which laid a foundation for breeding new varieties with disease resistance.

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Section: Introductionmentioning

confidence: 99%

Physiological response of CmWRKY15-1 to chrysanthemum white rust based on TRV-VIGS

Chen

Kuang²,

Wu³

et al. 2023

Front. Plant Sci.

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Metyltetraprole activity against plant pathogens with relatively rare cytochrome b haplotypes for azoxystrobin resistance

et al. 2022

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Metyltetraprole is a novel quinone outside inhibitor (QoI) fungicide designed to avoid cross-resistance in cytochrome b G143A-harboring QoI-resistant phytopathogenic fungi. The resistance factors of G143A-harboring fungal isolates for metyltetraprole are around 1, but > 200 for the reference QoI fungicide azoxystrobin. In this study of metyltetraprole activities against azoxystrobin-resistant isolates carrying G137R, G137S, L299F, N256S + L299F, or L275F + L299F in cytochrome b, metyltetraprole had potent activity against all isolates with these cytochrome b haplotypes. The resistance factors ranged from 0.7 to 2.9 for metyltetraprole and from 3.0 to 175.1 for azoxystrobin. We revealed unique metyltetraprole inhibitory activities against QoI-resistant plant pathogens.

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Sequence analysis of cytochrome b gene in Vietnamese isolates of Hemileia vastatrix, the causal agent of coffee leaf rust, in relation to potential QoI fungicide resistance

Ishii²,

Okane³

et al. 2023

Trop. plant pathol.

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New cytochrome b haplotypes, harboring L299F or N256S + L299F substitutions, were found in azoxystrobin-resistant Puccinia horiana, the causal agent of chrysanthemum white rust

Cited by 5 publications

References 19 publications

Physiological response of CmWRKY15-1 to chrysanthemum white rust based on TRV-VIGS

Physiological response of CmWRKY15-1 to chrysanthemum white rust based on TRV-VIGS

Metyltetraprole activity against plant pathogens with relatively rare cytochrome b haplotypes for azoxystrobin resistance

Sequence analysis of cytochrome b gene in Vietnamese isolates of Hemileia vastatrix, the causal agent of coffee leaf rust, in relation to potential QoI fungicide resistance

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