New Cycloartane-type Triterpenes fromMarcetia Latifolia(Melastomataceae) andIn SilicoStudies onCandida ParapsilosisProtease

Abstract

We have previously reported the antimicrobial activity of the ethyl acetate extract of Marcetia latifolia, particularly against Candida parapsilosis. In this work we describe the isolation of two new cycloartane-type triterpenes, 28,29-bis-norcycloartan-3β,4α-diol (1) and 28,29-bis-norcycloart-24-en-3β,4α-diol (2) from the same extract. These compounds were mainly characterized by one-(1 H, 13 C and APT) and two-dimensional (1 H-1 H-COSY, 1 H-1 H-NOESY, HMQC and HMBC) NMR spectroscopy, mass spectrometry and comparison with published structural data. In addition, the activity of triterpenes 1 and 2 on the Candida protease target was investigated by in silico methods using molecular docking.