New CTX-M Group Conferring β-Lactam Resistance: A Compendium of Phylogenetic Insights from Biochemical, Molecular, and Structural Biology

Mendonça, Jacinta; Silva, Carina; Sá, Sara; Oliveira, M. D. de; Accioly, Gustavo; Baylina, Pilar; Barata, Pedro; Pereira, Cláudia; Fernandes, Rúben

doi:10.3390/biology11020256

Cited by 8 publications

(10 citation statements)

References 41 publications

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“…The blaCTX-M-1_CV1 and blaCTX-M-1_CV2 subvariants were present in the same species as blaTEM-1_TV2. Previous studies found five key substitutions among CTX-M-1 variants: Val80Ala, Asp117Asn, Ser143Ala, Asp242Gly, and Asn289Asp [43] the first one being consistent with the variants found in this study. This is also the first report for blaOXA-181 detection from non-hospital settings in Ecuador.…”

Section: Discussionsupporting

confidence: 92%

First Screening of Beta-Lactam Antibiotic Resistance Genes and Bacterial Diversity in the Public Transport System of Quito, Ecuador

Hernández-Alomía¹,

Bastidas-Caldes²,

Ballesteros³

et al. 2022

Preprint

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Background: Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through mec genes, which encode modified penicillin-binding proteins (PBP2). This study aimed to determine the presence and spread of β-lactam antibiotic resistance genes and the microbiome circulating in Quito’s Public Transport (QTP). Methods: A total of 29 station turnstiles were swabbed to extract the surface environmental DNA. PCRs were performed to detect the presence of 13 antibiotic resistance genes and to identify 16S rDNA barcoding, followed by clone analysis, Sanger sequencing and BLAST search. Results: ESBL genes blaTEM-1 and blaCTX-M-1 and MBL genes blaOXA-181 and mecA were detected along QPT stations. Two subvariants were found for blaTEM-1, blaCTX-M-1, and blaOXA-181. Almost half of the circulating bacteria found at QPT stations were common human microbiota species including those classified by the WHO as pathogens of critical and high-priority surveillance. Conclusions: β-lactam antibiotic resistance genes are widely spread throughout QPT. This is the first report of blaOXA-181 in environmental samples in Ecuador. Moreover, we detected a new putative variant of this gene. Some commensal coagulase-negative bacteria may have a role as mecA resistance reservoirs

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Section: Discussionsupporting

confidence: 92%

First Screening of Beta-Lactam Antibiotic Resistance Genes and Bacterial Diversity in the Public Transport System of Quito, Ecuador

Hernández-Alomía¹,

Bastidas-Caldes²,

Ballesteros³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The bla CTX-M-1_ CV1 and bla CTX-M-1_ CV2 subvariants were present in the same species as bla TEM-1_ TV2. Previous studies found five key substitutions among CTX-M-1 variants: Val80Ala, Asp117Asn, Ser143Ala, Asp242Gly, and Asn289Asp [42], with the first one being consistent with the variants found in this study. This is also the first report for the detection of bla OXA-181 from a non-hospital environment in Ecuador.…”

Section: Discussionsupporting

confidence: 91%

Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador

Hernández-Alomía

Bastidas-Caldes

Ballesteros

et al. 2023

IJERPH

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Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through mec genes, which encode modified penicillin-binding proteins (PBP2). This study aimed to determine the presence and spread of β-lactam antibiotic resistance genes and the microbiome circulating in Quito’s Public Transport (QTP). A total of 29 station turnstiles were swabbed to extract the surface environmental DNA. PCRs were performed to detect the presence of 13 antibiotic resistance genes and to identify and to amplify 16S rDNA for barcoding, followed by clone analysis, Sanger sequencing, and BLAST search. ESBL genes blaTEM-1 and blaCTX-M-1 and MBL genes blaOXA-181 and mecA were detected along QPT stations, blaTEM being the most widely spread. Two subvariants were found for blaTEM-1, blaCTX-M-1, and blaOXA-181. Almost half of the circulating bacteria found at QPT stations were common human microbiota species, including those classified by the WHO as pathogens of critical and high-priority surveillance. β-lactam antibiotic resistance genes are prevalent throughout QPT. This is the first report of blaOXA-181 in environmental samples in Ecuador. Moreover, we detected a new putative variant of this gene. Some commensal coagulase-negative bacteria may have a role as mecA resistance reservoirs.

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“…While TEM and SHV types were predominant in the 1980s and 1990s, CTX-M has progressively become the most prevalent ESBL type. CTX-M is further categorized into six phylogenetic groups (CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, CTX-M-25, and CTX-M-151) based on amino acid sequence [ 8 ]. CTX-M groups 1 (e.g., CTX-M-1 and CTX-M-15) and 9 (e.g., CTX-M-14 and CTX-M-27) are the most commonly described groups, with CTX-M-15 being the most widespread variant among clinical Enterobacterales worldwide [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Types of Extended-Spectrum β-Lactamase-Producing Bacteria in Retail Seafood

Pearce

Conrady

Guardabassi

2023

Foods

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Objectives: To assess prevalence and types of extended-spectrum β-lactamase (ESBL)-producing bacteria in retail seafood. Methods: A literature review was completed according to international guidelines for systematic reviews, except for being performed by a single reviewer. Kruskal–Wallis and Dunn tests were used to determine statistical differences between continents or seafood types. Results: Among 12,277 hits, 42 publications from 2011 to 2023 were deemed relevant to the review’s objectives. The median prevalence of ESBL-contaminated products was 19.4%. A significantly lower prevalence was observed in Europe (p = 0.006) and Africa (p = 0.004) compared to Asia. Amongst the 2053 isolates analyzed in the selected studies, 44.8% were ESBL-positive. The predominant type was CTX-M (93.6%), followed by TEM (6.7%) and SHV (5.0%). Only 32.6% and 18.5% of the CTX-M-positive isolates were typed to group and gene level, respectively. While group 1 (60.2%) was prevalent over group 9 (39.8%) among Enterobacterales, the opposite trend was observed in Vibrio spp. (60.0% vs. 40.0%). Information at gene level was limited to Enterobacterales, where CTX-M-15 was the most prevalent (79.2%). Conclusions: On average, one in five seafood products sold at retail globally is contaminated with ESBL-producing Enterobacterales of clinical relevance. Our findings highlight a potential risk for consumers of raw seafood, especially in Asia.

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New CTX-M Group Conferring β-Lactam Resistance: A Compendium of Phylogenetic Insights from Biochemical, Molecular, and Structural Biology

Cited by 8 publications

References 41 publications

First Screening of Beta-Lactam Antibiotic Resistance Genes and Bacterial Diversity in the Public Transport System of Quito, Ecuador

First Screening of Beta-Lactam Antibiotic Resistance Genes and Bacterial Diversity in the Public Transport System of Quito, Ecuador

Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador

Prevalence and Types of Extended-Spectrum β-Lactamase-Producing Bacteria in Retail Seafood

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