New corrosion cast media and its ability for SEM and light microscope investigation

Tsomaia, Keti; Patarashvili, Leila; Bebiashvili, Irakli; Azmaiparashvili, Elza; Kakabadze, Manana; Jalabadze, Nikoloz; Sareli, Merab; Гусев, С. А.; Kordzaia, Dimitri

doi:10.1002/jemt.23468

Cited by 5 publications

(7 citation statements)

References 23 publications

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“…The injection was performed under diethyl ether deep general anesthesia. Both the injection technique of the solidifying mass and the corrosion of liver tissue were carried out in accordance with a previously described technique (Tsomaia et al, 2020). The manipulations were performed using microsurgical microscopy (Bino Scientific, Manufacturer: Nature of Business, India ISO 9001–2008).…”

Section: Methodsmentioning

confidence: 99%

“…We obtained corrosion casts by filling the tubular structures with various solidifying masses and through subsequent corrosion of the organs in acid or alkali. These casts are elastic and undamaged and can be stored for a long period (practically imperishable) and can be subjected to spatial study (including stereomicroscopes) and measurement of various parameters (Krucker, Lang, & Meyer, 2006; Tsomaia et al, 2020). All of the above criteria were met by three‐dimensional computed tomography images.…”

Section: Introductionmentioning

confidence: 99%

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Spatial architecture of biliary tree in mammals: Fractal and Euclidean geometric features

Azmaiparashvili¹,

Patarashvili²,

Bebiashvili³

et al. 2021

Journal of Anatomy

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The study of the fractal architecture of various organs and structures expanded the possibilities for determining the ranges of their functioning and structural arrangement, which, as a result, was reflected in the development of new approaches to diagnostics and therapeutic impacts. The architecture of the excretory duct systems, similar to the hemo‐ and lymph‐ circulatory beds and the bronchial tree, is considered fractal. At the same time, information about hitherto unknown structures of the biliary tree continues to appear in the literature. We aimed to study the features of the spatial geometry of the biliary tree and assess the significance of both its fractal and Euclidean characteristics for the development of approaches that facilitate comprehensive description of intrahepatic biliary tract architecture. We investigated the architecture of the biliary trees of six men, seven male canines, and seven male Wistar rats using the corrosion casting method. Corrosion casts were prepared by injecting solidifying latexes into the bile ducts. The preparations were studied using a light stereomicroscope and a scanning electron microscope. Biliary tree branching is of various types. In addition, the correlation between variations in the caliber and length of the bile ducts and their order is not significant. Therefore, the biliary tree should not be considered as a classical fractal and it consists of the main modules, represented by the network of the bile canaliculi (first nonfractal module) and a biliary tree with a fractal branching (second module) that drains the bile canaliculi mesh and the additional modules represented by the mucosal biliary glands (in mammals with the gallbladder) or the periportal biliary plexus (in mammals without a gallbladder) and the aberrant biliary ducts. Such a configuration of the biliary bed should optimally ensure the smooth implementation of the physiological function of the liver, as well as its adaptation to different pathologies accompanied by biliary hypertension. It also might be considered in the diagnosis and assessment of ductular reaction, biliary regeneration, and/or carcinogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatial architecture of biliary tree in mammals: Fractal and Euclidean geometric features

Azmaiparashvili¹,

Patarashvili²,

Bebiashvili³

et al. 2021

Journal of Anatomy

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“…The solidifying mass obtained by the mixture of 0.25 g MAYCRYL C. C. powder (4.7% of whole mass) + 0.08 g benzoyl peroxide (1.5% of whole mass) + 5.0 mL protacryl-M liquid component was injected into the liver blood circulatory network through the portal vein of the Wistar rats[ 34 ]. The injection was carried out under diethyl ether deep general anesthesia, after washing-out of the blood vessels with 0.1 mL.…”

Section: Methodsmentioning

confidence: 99%

“…Heparin (5000 un/mL) mixed in 0.9% NaCl solution (washing-out speed – 20 mL/min). The details of the injection were described earlier[ 35 ] ISM was injected by using a manometer-syringe of the own construction[ 34 ]. The injection pressure varied between 20-30 mmHg.…”

Section: Methodsmentioning

confidence: 99%

Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration

Tsomaia

Patarashvili

Karumidze

et al. 2020

WJG

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BACKGROUND The phenomenon of liver regeneration after partial hepatectomy (PH) is still a subject of considerable interest due to the increasing frequency of half liver transplantation on the one hand, and on the other hand, new surgical approaches which allow removal of massive space-occupying hepatic tumors, which earlier was considered as inoperable. Interestingly, the mechanisms of liver regeneration are extensively studied after PH but less attention is paid to the architectonics of the regenerated organ. Because of this, the question “How does the structure of regenerated liver differ from normal, regular liver?” has not been fully answered yet. Furthermore, almost without any attention is left the liver's structural transformation after repeated hepatectomy (of the re-regenereted liver). AIM To compare the architectonics of the lobules and circulatory bed of normal, re-generated and re-regenerated livers. METHODS The livers of 40 adult, male, albino Wistar rats were studied. 14 rats were subjected to PH - the 1 st study group (SG1); 10 rats underwent repeated PH – the 2 nd study group (SG2); 16 rats were subjected to sham operation - control group (CG); The livers were studied after 9 months from PH, and after 6 months from repeated PH. Cytological (Schiff reaction for the determination of DNA concen-tration), histological (H&E, Masson trichrome, CK8 Immunohistochemical marker, transparent slides after Indian Ink injection, ), morphometrical (hepatocytes areas, perimeters and ploidy) and Electron Microscopical (Scanning Electron Microscopy of corrosion casts) methods were used. RESULTS In the SG1 and SG2, the area of hepatocytes and their perimeter are increased compared to the CG ( P < 0.05). However, the areas and perimeters of the hepatocytes of the SG 1 and SG 2 groups reveal a lesser difference. In regenerated (SG 1 ) and re-regenerated (SG 2 ) livers, the hepatocytes form the remodeled lobules, which size (300-1200 µm) exceeds the sizes of the lobules from CG (300-600 µm). The remodeled lobules (especially the “mega-lobules” with the sizes 1000-1200 µm) contain the transformed meshworks of the sinusoids, the part of which is dilated asymmetrically. This meshwork might have originated from the several portal venules (interlobular and/or inlet). The boundaries between the adjacent lobules (including mega-lobules) are widened and filled by connective tissue fibers, which gives the liver parenchyma a nodular look. In SG 2 the unevenness of sinusoid diameters, as well as the boundaries between the lobules (including the mega-lobules) are more vividly expressed in comparison with SG 1 . The liver tissue of both SG 1 and SG 2...

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“…There is a representation of a network of blood vessels. The major challenge is to completely perfuse such a system so that the fluid can effectively pass the bed of fine capillary vessels connecting both sides 34,35 . A casting material that is being injected will travel through the vessels at a specific rate, which will translate into a velocity profile, with each vessel possessing a different diameter.…”

Section: Macroscopic Tools and Criteria For Casting Medium Selectionmentioning

confidence: 99%

From identifying polymeric resins to corrosion casting applications

Reyes

D’hooge

Cornillie

2022

J of Applied Polymer Sci

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Corrosion casting is an important manufacturing technique to replicate anatomical structures. Its execution is often based on the injection of resins that can be polymerized starting at room temperature in the presence of an accelerator, which suits most of the practical requirements. In the present contribution, we highlight the main challenges of the corrosion casting technique, addressing a multidisciplinary research angle connecting the fields of life science, veterinary science, materials science, rheology, and polymer reaction engineering. We pay attention to (commercial) material availability, casting medium selection criteria, specimen preparation, maceration, injection possibilities, cleaning procedures, and detail of replication. We start the discussion from general principles and recommendations to then include major examples for each corrosion casting characteristic addressed.

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New corrosion cast media and its ability for SEM and light microscope investigation

Cited by 5 publications

References 23 publications

Spatial architecture of biliary tree in mammals: Fractal and Euclidean geometric features

Spatial architecture of biliary tree in mammals: Fractal and Euclidean geometric features

Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration

From identifying polymeric resins to corrosion casting applications

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