New concepts in anammox processes for wastewater nitrogen removal: recent advances and future prospects

González‐Martínez, Alejandro; Muñoz-Palazon, Bárbara; Rodríguez-Sánchez, Alejandro; González‐López, Jesús

doi:10.1093/femsle/fny031

Cited by 47 publications

(23 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high environmental sensitivity of AnAOB brings many obstacles to mainstream application. Researchers have discovered that the operational parameters (temperature, dissolved oxygen, pH), organics, antibiotics, salts, heavy metals, and even excess ammonia and nitrite have certain adverse effects on the growth and activity of AnAOB (Gonzalez‐Martinez, Muñoz‐Palazon, Rodriguez‐Sanchez, & Gonzalez‐Lopez, 2018; Nsenga Kumwimba, Lotti, Şenel, Li, & Suanon, 2020). Nitrogen is mainly present in the form of ammonium in raw municipal wastewater, and nitrite is strictly limited.…”

Section: Crucial Factors For the Stable Operation Of Anammox Bioreactmentioning

confidence: 99%

Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox‐based processes

Huang

et al. 2020

Water Environment Research

View full text Add to dashboard Cite

Anaerobic ammonium oxidation (anammox) is a novel process of deammonification that exhibits superior ecological and economic potential compared to that of traditional heterotrophic processes. Although this process has been successfully implemented in treating high‐strength nitrogen‐contaminated wastewater, it still faces many challenges in treating mainstream municipal wastewater. This review aims to provide an overview of the status and challenges of mainstream anammox‐based processes. The different configurations and crucial factors are discussed in this review. Finally, the future needs for feasible application are stated. Practitioner points Factors restricting mainstream application of anammox‐based processes are reviewed. Control strategies for selecting and maintaining anammox bacteria are discussed. Recent advances in nitrite production via partial nitrification or denitrification are summarized. Future needs for the feasible application of anammox‐based nitrogen removal technology for mainstream municipal wastewater treatment are outlined.

show abstract

Section: Crucial Factors For the Stable Operation Of Anammox Bioreactmentioning

confidence: 99%

Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox‐based processes

Huang

et al. 2020

Water Environment Research

View full text Add to dashboard Cite

show abstract

“…Therefore, controlling pH closely coordinates the efficiency of nitrogen removal of anammox reactors. The implementation of anammox in a single bioreactor is a novel approach that can remove the nitrogen from both municipal and industrial wastewaters by controlling pH and dissolved oxygen through aeration [33,77]. The single-stage anammox process can achieve an energy-cost saving of 25% compared to the conventional process provided that stability of pH and aeration is controlled [59,77].…”

Section: Phmentioning

confidence: 99%

“…This suggested that the application of anammox in an environment with moderate DO level in the presence of symbiont bacteria could be considered. Controlling the DO levels in the reactor would support the oxidation of ammonium to nitrite and prevent the oxidation of nitrite to nitrate, which is challenging for the anammox reaction [77,87].…”

Section: Dissolved Oxygen (Do)mentioning

confidence: 99%

“…The common AOB found in the anammox system were Nitrosomonas eutropha, N. europaea, and Nitrosospira sp. [77]. Nitrosomonas was reported to be the dominant genus of AOB.…”

Section: Dissolved Oxygen (Do)mentioning

confidence: 99%

“…Anammox symbionts can typically oxidize a multitude of organic compounds with simultaneous reduction in nitrate and/or nitrite [97][98][99][100][101]. During the anammox operation, cell lysis or solubilization of unwanted material in the system resulted in the increase of organic matter from 6 ± 2 mg/L to 327 ± 21 mg/L, and this directly reduced the performance of anammox bacteria [77]. The study by García-Ruiz et al [102] revealed that the nitrogen removal efficiency was not affected by the organic carbon level of 100 mg COD (chemical oxygen demand) L −1 during the operation of a CANON biofilter system in which coexisting AOB, anammox, and denitrifiers were fed with an influent with an ammonium concentration of 320 mg/L.…”

Section: Carbon Sourcesmentioning

confidence: 99%

See 2 more Smart Citations

Performance of Anammox Processes for Wastewater Treatment: A Critical Review on Effects of Operational Conditions and Environmental Stresses

Cho

Kambey

Nguyễn

2019

Water

116

View full text Add to dashboard Cite

The anaerobic ammonium oxidation (anammox) process is well-known as a low-energy consuming and eco-friendly technology for treating nitrogen-rich wastewater. Although the anammox reaction was widely investigated in terms of its application in many wastewater treatment processes, practical anammox application at the pilot and industrial scales is limited because nitrogen removal efficiency and anammox activity are dependent on many operational factors such as temperature, pH, dissolved oxygen concentration, nitrogen loading, and organic matter content. In practical application, anammox bacteria are possibly vulnerable to non-essential compounds such as sulfides, toxic metal elements, alcohols, phenols, and antibiotics that are potential inhibitors owing to the complexity of the wastewater stream. This review systematically summarizes up-to-date studies on the effect of various operational factors on nitrogen removal performance along with reactor type, mode of operation (batch or continuous), and cultured anammox bacterial species. The effect of potential anammox inhibition factors such as high nitrite concentration, high salinity, sulfides, toxic metal elements, and toxic organic compounds is listed with a thorough interpretation of the synergistic and antagonistic toxicity of these inhibitors. Finally, the strategy for optimization of anammox processes for wastewater treatment is suggested, and the importance of future studies on anammox applications is indicated.

show abstract

Purification of the key enzyme complexes of the anammox pathway from DEMON sludge

et al. 2021

View full text Add to dashboard Cite

Anaerobic Ammonium Oxidation (“anammox”) is a bacterial process in which nitrite and ammonium are converted into nitrogen gas and water, yielding energy for the cell. Anammox is an important branch of the global biological nitrogen cycle, being responsible for up to 50% of the yearly nitrogen removal from the oceans. Strikingly, the anammox process uniquely relies on the extremely reactive and toxic compound hydrazine as a free intermediate. Given its global importance and biochemical novelty, there is considerable interest in the enzymes at the heart of the anammox pathway. Unfortunately, obtaining these enzymes in sufficiently large amounts for biochemical and structural studies is problematic, given the slow growth of pure cultures of anammox bacteria when high cell densities are required. However, the anammox process is being applied in wastewater treatment to remove nitrogenous waste in processes like DEamMONification (DEMON). In plants using such processes, which rely on a combination of aerobic ammonia‐oxidizers and anammox organisms, kilogram amounts of anammox bacteria‐containing sludge are readily available. Here, we report a protein isolation protocol starting from anammox cells present in DEMON sludge from a wastewater treatment plan that readily yields pure preparations of key anammox proteins in the tens of milligrams, including hydrazine synthase HZS and hydrazine dehydrogenase (HDH), as well as hydroxylamine oxidoreductase (HAO). HDH and HAO were active and of sufficient quality for biochemical studies and for HAO, the crystal structure could be determined. The method presented here provides a viable way to obtain materials for the study of proteins not only from the central anammox metabolism but also for the study of other exciting aspects of anammox bacteria, such as for example, their unusual ladderane lipids.

show abstract

New concepts in anammox processes for wastewater nitrogen removal: recent advances and future prospects

Cited by 47 publications

References 98 publications

Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox‐based processes

Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox‐based processes

Performance of Anammox Processes for Wastewater Treatment: A Critical Review on Effects of Operational Conditions and Environmental Stresses

Purification of the key enzyme complexes of the anammox pathway from DEMON sludge

Contact Info

Product

Resources

About