New combinations and new species of Solenostoma and Plectocolea (Solenostomataceae) from the Russian Far East

Taxonomic Novelties in Solenostoma - Plectocolea Complex (Solenostomataceae, Hepaticae) in East Asia

“…The enlarged dataset analyzed in this study leads to changes in tree topologies compared with those in Bakalin & Vilnet (2012). There are four main clades with par tial ly weak supported relation (Fig.…”

“…In the course of exploration of Solenostoma -Plectocolea complex we revealed some new for science taxa or re-eva luated epithets regarded before as synonyms (Bakalin & Vilnet 2012, Bakalin 2013. Recently we had an opportunity to involve new material into the analysis to make further cla rification in this complex, one of the most difficult ta xonomic entities in temperate East Asia, that resulted in some no men clatural changes as well as the creation of two new ge nera.…”

“…Newly generated nucleotide sequences were analyzed with previously published data for 86 specimens. Species of the genus Marsupella were chosen as an outgroup (Bakalin & Vilnet 2012). The samples studied here are listed in Table 1 with GenBank accession numbers and voucher details.…”

“…Nucleotide data of trnL-F and trnG-intron of each sequen ced specimen were combined and included in produced earlier alignment for 86 specimens from genera Solenostoma and Plectocolea (Bakalin & Vilnet 2012) using BioEdit v. 7.0.1 (Hall 1999); all positions of the alignment were included in the phylogenetic analysis. The infrageneric and infraspecific va riability of each DNA locus was evaluated as the value of the p-distances between samples and species of each ge nera, as calculated in Mega 5.1 (Tamura et al 2011) using the pairwise deletion option for counting gaps.…”

“…Unexpected molecular diver gence together with morphological uniqueness allows us to elevate section Protosolenostoma with this species to ge ne ric rank (see below). The second diverged clade was pre sen ted by S. ochotensis, S. gracillima, S. fusiforme (as treated in Bakalin & Vilnet 2012) and specimens identified as P. rub ri punctata (Japan) (BS=94 % in MP, BS=100 % in ML, PP=1.00 in BA) from main Solenostoma-clade. Relation of this clade to Plectocolea and Solenostoma was supported by BS=77 % in MP and PP=1.00 in BA compared with supports of its previous affinity to Solenostoma-clade BS=59 % in MP, BS=71 % in ML, PP=0.69 in BA in Baka lin & Vilnet (2012: Figs.…”