New, Clinically More Relevant Model for Nerve Root Injury in the Rat

Finskas, Oscar; Blixt, Åsa; Fujioka, Yuki; Olmarker, Kjell

doi:10.1097/brs.0b013e3182a0d264

Cited by 1 publication

(5 citation statements)

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“…The newly developed rat model used in this study has been shown to increase spontaneous pain-related behavior [4], but studies specifically evaluating hyperalgesia have not yet been conducted, which is a limitation when comparing our results with others. Nevertheless, we believe this is the most clinically relevant model available, because the needle induces mechanical deformation of the clinically most commonly affected structure (the nerve root) [4].…”

Section: Discussionmentioning

confidence: 52%

“…In this pilot study, we found a downregulation of the Htr2c gene in experimental disc herniation in the rat that was reduced by TNF inhibition. This suggests that changes in serotonergic transmission may contribute to the changes in spontaneous pain behavior previously observed in this model [4], shedding further light on the complex pathophysiology of disc herniation and sciatica.…”

Section: Discussionmentioning

confidence: 95%

“…Nevertheless, we believe this is the most clinically relevant model available, because the needle induces mechanical deformation of the clinically most commonly affected structure (the nerve root) [4]. Also, changes in gene expression, in this study primarily receptor genes, are believed to reflect a change in the number of receptors on the cell surface with downregulation causing lowered receptor-mediated signaling and vice versa.…”

Section: Discussionmentioning

confidence: 99%

“…1) [4]. All animal procedures were carried out following the Principles of Laboratory Animal Care and were approved by the local animal research ethics committee.…”

Section: Methodsmentioning

confidence: 99%

“…In the experimental disc herniation group (DH) (n = 5) [4], after exposure of the nerve root, dorsal root ganglion, and intervertebral disc as described in the sham group, the L3-L4 disc was punctured using a 0.4-mm injection needle. A small amount of air was injected into the disc to facilitate leakage of nucleus pulposus.…”

Section: Surgical Proceduresmentioning

confidence: 99%

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Experimental Disc Herniation in the Rat Causes Downregulation of Serotonin Receptor 2c in a TNF-dependent Manner

Jonsson

Finskas

Fujioka

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background During recent decades, the knowledge of the pathophysiology of disc herniation and sciatica has drastically improved. What previously was considered a strict biomechanical process is now considered a more complex interaction between leaked nucleus pulposus and the tissue in the spinal canal. An inflammatory reaction, with tumor necrosis factor (TNF) playing an essential role, has been demonstrated. However, the exact mechanisms of the pathophysiology of disc herniation remain unknown. Questions/purposes In this study we use an animal model to investigate (1) if and/or how experimental disc herniation affects gene expression in the early phase (24 hours postsurgery) in the dorsal root ganglion; and (2) if TNF inhibition can reduce any observed changes. Methods A rat model of disc herniation was used. Twenty rats were evenly divided into four groups: naïve, sham, disc herniation, and disc herniation with TNF inhibition. The dorsal root ganglion of the affected nerve root was harvested 24 hours after surgery and analyzed with a TaqMan Low Density Array 1 quantitative polymerase chain reaction assay. Gene expression levels in sham were compared with disc herniation to assess question 1 and disc herniation to disc herniation with TNF inhibition to assess question 2. Results Experimental disc herniation caused a decrease in the expression of the serotonin receptor 2c gene (p = 0.022). TNF inhibition was found to reduce the observed decrease in expression of serotonin receptor 2c (p = 0.037). Conclusions Our results suggest that a decrease in the expression of the serotonin receptor 2c gene may contribute to the pathophysiology of disc herniation. Further research on its involvement is warranted. Clinical Relevance This pilot study gives a brief insight into cellular changes that may contribute to the pathophysiology of disc herniation. This knowledge may

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

“…1) [4]. All animal procedures were carried out following the Principles of Laboratory Animal Care and were approved by the local animal research ethics committee.…”

Section: Methodsmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

See 3 more Smart Citations