New chiral high-performance liquid chromatographic methodology used for the pharmacokinetic evaluation of dexfenfluramine

Zeng, Jianing; Li, Dou; Duda, Michael P.; Stuting, Hans H.

doi:10.1016/0378-4347(94)00004-2

Cited by 12 publications

(2 citation statements)

References 17 publications

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“…Several methods have been reported for the quantitative determination of Fen and Phen using gas chromatography (GC) (Richards et al, 1989;Brownsill et al, 1991;Morris and Reece, 1983;Franceschini et al, 1991) or high performance liquid chromatography (HPLC) (Zeng et al, 1994;Gross et al, 1993;Bogusz et al, 1997;Jonsson et al, 1996;Beckett and Brookes, 1971;O'Brien et al, 1972;Clausing et al, 1997). In most of these methods the determination of Fen and Phen was achieved separately with a minimum volume of 1 mL of either plasma or urine sample.…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4‐(4,5‐diphenyl‐1H‐imidazole‐2‐yl)benzoyl chloride as a label

Kaddoumi

Kubota

Nakashima

et al. 2001

Biomedical Chromatography

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A high performance liquid chromatographic method has been developed for the simultaneous determination of (+/-) fenfluramine (Fen) and phentermine (Phen) in addition to three other sympathomimetic amines-ephedrine (E), norephedrine (NE) and 2-phenylethylamine (2-PEA), using cyclohexylamine (CX) as an internal standard in plasma. The compounds were derivatized with 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride (DIB-Cl) to give the DIB-derivatives. The derivatives were then separated using an isocratic HPLC system with UV detection. The limits of detection for Fen, Phen, E, NE and 2-PEA in plasma ranged from 0.32 to 22.9 pmol on column at a signal-to-noise ratio of 3. The recoveries following alkaline extraction from plasma samples of known concentrations were found to be more than 94% for the studied compounds. This method might be useful for the screening of the studied sympathomimetic amines in human plasma samples in forensic as well as toxicological studies. Furthermore, the developed method was modified for the simultaneous determination of Fen and Phen in human and rat plasma using fluoxetine as an internal standard. The methods are reproducible and precise. Finally, the two drugs were administered intraperitoneally to rats in combination, and their plasma levels over the investigated time course were successfully determined.

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Section: Introductionmentioning

confidence: 99%

High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4‐(4,5‐diphenyl‐1H‐imidazole‐2‐yl)benzoyl chloride as a label

Kaddoumi

Kubota

Nakashima

et al. 2001

Biomedical Chromatography

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“…Several methods have been developed for determining Phen and Fen in biological fluids, using either gas chromatography (GC) (3)(4)(5)(6) or high-performance liquid chromatography (HPLC) (7)(8)(9)(10)(11)(12). In these methods, the minimum sample volume used for their quantitation in plasma or urine was 1 mL with minimum quantitation limits of 10 ng/mL (67 nM) and 2.5 ng/mL (11 nM) for Phen (12) and Fen (3), respectively.…”

Section: Introductionmentioning

confidence: 99%

HPLC of (±)-Fenfluramine and Phentermine in Plasma After Derivatization With Dansyl Chloride

Kaddoumi

Nakashima

Wada

et al. 2001

Journal of Liquid Chromatography & Related Technologies

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A high-performance liquid chromatographic method is described for the simultaneous determination of the dansyl derivatives of (±)-fenfluramine (Fen) and phentermine (Phen) in addition to four other sympathomimetic amines, namely, norephedrine (NE), ephedrine (E), 2-phenylethylamine (2-PEA), and 4-bromo-2,5-dimethoxyphenethylamine (2-CB), using fluoxetine (FLX) as an internal standard. The separation was performed on a reversedphase C 18 column, using a gradient system and fluorescence detection. The dansylation conditions were examined and the optimum ORDER REPRINTS 58KADDOUMI ET AL.derivatization was obtained at pH 9.0 with 4.7 mM dansyl chloride concentration. A preliminary study for the simultaneous determination of the dansyl derivatives in spiked human plasma was performed. The derivatives were well separated within 45 min and showed good linearities from 0.005 to 2µM for E, Phen, and Fen, from 0.002 to 0.8 µM for 2-CB, and from 0.025 to 2 µM for NE and 2-PEA with detection limits ranging from 16 to 255 fmol on the column. Extraction recovery of the compounds was greater than 90%. The applicability of the method was studied in rat plasma following an intraperitoneal administration of Fen and Phen.

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Chiral high-performance liquid chromatography methodology for quality control monitoring of dexfenfluramine

Li¹,

Zeng²,

Gerochi³

et al. 1994

Journal of Chromatography A

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New chiral high-performance liquid chromatographic methodology used for the pharmacokinetic evaluation of dexfenfluramine

Cited by 12 publications

References 17 publications

High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4‐(4,5‐diphenyl‐1H‐imidazole‐2‐yl)benzoyl chloride as a label

High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4‐(4,5‐diphenyl‐1H‐imidazole‐2‐yl)benzoyl chloride as a label

HPLC of (±)-Fenfluramine and Phentermine in Plasma After Derivatization With Dansyl Chloride

Chiral high-performance liquid chromatography methodology for quality control monitoring of dexfenfluramine

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