New building blocks or dendritic pseudopeptides for metal chelating

Abstract

Dendritic oligopeptides have been reported as useful building blocks for many interactions. Starting from hydrazine, we described an approach to create new dendritic pseudopeptides linked with biological systems, such as cell membrane, as chelate metal, Ni2+-nitrilotriacetic acid moieties which could target histidine rich peptides or proteins. Depending on the nature of these new chemical recognition units, they could be integrated into a peptide by coupling in C or N-termini.Graphical abstract:Dendrimer formation

Biosensors Based on the Binding Events of Nitrilotriacetic Acid–Metal Complexes

“…The entrapped metal ions serve as Lewis acids and the remained unoccupied coordination sites can further ligate to the imidazole moieties of hexahistidine (His 6 ) tags. As coordination-bonding-based artificial receptors, the ligands exhibit different IMAC protein separation efficiencies based on the number and conformation of chelation sites [18,19]. Among them, the tetradentate ligand of NTA can be chelated with bivalent transition metal cations (e.g., Cu 2+ , Ni 2+ , Zn 2+ and Co 2+ ) to form a hexagonal complex and two unoccupied coordination sites remain for the further ligation to the imidazole moieties of His 6 tag.…”