New Biomarkers of Sepsis with Clinical Relevance

Cunha, Débora Maria da Gomes; Silva, Guilherme Galdino da; Hamasaki, Mike Yoshio

doi:10.5772/intechopen.82156

Cited by 5 publications

(2 citation statements)

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“…Besides symptoms and signs for the early diagnosis of sepsis, available biomarkers are important for the following treatment of sepsis. Some biological markers have been established such as lactate, C-reactive protein (CRP), procalcitonin (PCT), B-type natriuretic peptide (BNP), and soluble RAGE (sRAGE) in sepsis, while lack of sensitivity and specificity limit their clinical application ( Oberhoffer et al, 1999 ; Phua et al, 2008 ; Hamasaki et al, 2014 ; Débora Maria da Gomes Cunha and Yoshio Hamasaki, 2019 ). It is of prime importance to identify more effective technologies and specific biomarkers for sepsis.…”

Section: Introductionmentioning

confidence: 99%

The Landscape of Featured Metabolism-Related Genes and Imbalanced Immune Cell Subsets in Sepsis

et al. 2022

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Sepsis is a heterogeneous disease state triggered by an uncontrolled inflammatory host response with high mortality and morbidity in severely ill patients. Unfortunately, the treatment effectiveness varies among sepsis patients and the underlying mechanisms have yet to be elucidated. The present aim is to explore featured metabolism-related genes that may become the biomarkers in patients with sepsis. In this study, differentially expressed genes (DEGs) between sepsis and non-sepsis in whole blood samples were identified using two previously published datasets (GSE95233 and GSE54514). A total of 66 common DEGs were determined, namely, 52 upregulated and 14 downregulated DEGs. The Gene Set Enrichment Analysis (GSEA) results indicated that these DEGs participated in several metabolic processes including carbohydrate derivative, lipid, organic acid synthesis oxidation reduction, and small-molecule biosynthesis in patients with sepsis. Subsequently, a total of 8 hub genes were screened in the module with the highest score from the Cytoscape plugin cytoHubba. Further study showed that these hub DEGs may be robust markers for sepsis with high area under receiver operating characteristic curve (AUROC). The diagnostic values of these hub genes were further validated in myocardial tissues of septic rats and normal controls by untargeted metabolomics analysis using liquid chromatography-mass spectrometry (LC-MS). Immune cell infiltration analysis revealed that different infiltration patterns were mainly characterized by B cells, T cells, NK cells, monocytes, macrophages, dendritics, eosinophils, and neutrophils between sepsis patients and normal controls. This study indicates that metabolic hub genes may be hopeful biomarkers for prognosis prediction and precise treatment in sepsis patients.

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Section: Introductionmentioning

confidence: 99%

The Landscape of Featured Metabolism-Related Genes and Imbalanced Immune Cell Subsets in Sepsis

et al. 2022

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“…Early diagnosis in order to early intervention before organ dysfunction is critical to improving survival rate of sepsis ( 4 ). Due to their poor sensitivity and specificity in assessing the substantial disease heterogeneity, the existing biological markers cannot be used for sepsis prognosis prediction ( 5 – 8 ). Therefore, further studies are required to investigate the pathogenesis of sepsis and identify more sensitive and specific therapeutic targets.…”

Section: Introductionmentioning

confidence: 99%

Integrative single-cell RNA sequencing and metabolomics decipher the imbalanced lipid-metabolism in maladaptive immune responses during sepsis

et al. 2023

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BackgroundTo identify differentially expressed lipid metabolism-related genes (DE-LMRGs) responsible for immune dysfunction in sepsis.MethodsThe lipid metabolism-related hub genes were screened using machine learning algorithms, and the immune cell infiltration of these hub genes were assessed by CIBERSORT and Single-sample GSEA. Next, the immune function of these hub genes at the single-cell level were validated by comparing multiregional immune landscapes between septic patients (SP) and healthy control (HC). Then, the support vector machine-recursive feature elimination (SVM-RFE) algorithm was conducted to compare the significantly altered metabolites critical to hub genes between SP and HC. Furthermore, the role of the key hub gene was verified in sepsis rats and LPS-induced cardiomyocytes, respectively.ResultsA total of 508 DE-LMRGs were identified between SP and HC, and 5 hub genes relevant to lipid metabolism (MAPK14, EPHX2, BMX, FCER1A, and PAFAH2) were screened. Then, we found an immunosuppressive microenvironment in sepsis. The role of hub genes in immune cells was further confirmed by the single-cell RNA landscape. Moreover, significantly altered metabolites were mainly enriched in lipid metabolism-related signaling pathways and were associated with MAPK14. Finally, inhibiting MAPK14 decreased the levels of inflammatory cytokines and improved the survival and myocardial injury of sepsis.ConclusionThe lipid metabolism-related hub genes may have great potential in prognosis prediction and precise treatment for sepsis patients.

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Neutrophil Lymphocyte Ratio can Preempt Development of Sepsis After Adult Living Donor Liver Transplantation

Sarin¹,

Pamecha²,

Sinha³

et al. 2022

Journal of Clinical and Experimental Hepatology

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New Biomarkers of Sepsis with Clinical Relevance

Abstract: A 2016 task force convened by multiple societies proposed a new definition of sepsis, termed Sepsis 3. The new clinical diagnosis of sepsis is based on variation points in the Sequential (Sepsis-related)

Cited by 5 publications

References 74 publications

The Landscape of Featured Metabolism-Related Genes and Imbalanced Immune Cell Subsets in Sepsis

The Landscape of Featured Metabolism-Related Genes and Imbalanced Immune Cell Subsets in Sepsis

Integrative single-cell RNA sequencing and metabolomics decipher the imbalanced lipid-metabolism in maladaptive immune responses during sepsis

Neutrophil Lymphocyte Ratio can Preempt Development of Sepsis After Adult Living Donor Liver Transplantation

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