New Biomarkers for Cervical Cancer – Perspectives from the IGF System

Serrano, M. Cruz Sánchez; Umaña‐Pérez, Adriana; Garay‐Baquero, Diana J.; Sánchez-Gómez, Myriam

doi:10.5772/28007

Cited by 1 publication

(3 citation statements)

References 78 publications

(75 reference statements)

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“…The IGF system consists of three ligands, IGF-I, IGF-II and insulin; three cell membrane receptors, IGF-I receptor (IGF-IR), insulin receptor (IR) and IGF-II receptor (IGF-IIR); and six high-affinity IGF binding proteins, IGFBP − 1 through −6, their specific proteases (IGFBP proteases) and membrane receptors (IGFBP-R). IGF-I is a potent mitogenic growth factor that plays a critical role in cancergenesis [64]. …”

Section: Discussionmentioning

confidence: 99%

“…Elevated CA 125 levels are detectable in 20% to 75% of patients with cervical adenocarcinoma and have been associated with advanced tumor stage, large tumor size, high histological grade, lymph node involvement and status [64]. …”

Section: Discussionmentioning

confidence: 99%

“…• Colposcopy : specific colposcopic signs of PVI are acetowhite epithelium, positive iodine punctuation and mosaic, atypical transformation zone [9], develop in vivo subcellular imaging, enhancing optical imaging techniques as quantum dots [64];…”

Section: Discussionmentioning

confidence: 99%

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Development of biomarker panel to predict, prevent and create treatments tailored to the persons with human papillomavirus-induced cervical precancerous lesions

et al. 2014

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IntroductionHuman papillomavirus (HPV) induce many cancer conditions and cause cervical cancer, second in frequency of malignant disease in women.The aim was to develop biomarker panel for HPV-induced cervical precancerous diseases in patients infected with herpes simplex virus (HSV).Material and methodsThe study involved 71 women with cervical precancerous diseases (mean age 26 ± 5 years) revealed by colposcopic, cytomorphological, and ultrasound signs which were assessed according to the following: first group, 44 patients infected with HPV; second group, 27 HPV-negative patients; and third group, 30 healthy patients (controls). In cervical specimen, we identified HPV DNA of different oncogenic risk types by polymerase chain reaction (PCR). Enzyme-linked immunosorbent assay (ELISA) kits (JSC SPC ‘DiaprofMed’) were used for detecting antibodies to HSV1 and/or HSV2 and for determining the avidity index. The production of pro-inflammatory cytokines, interferon-γ (IFN-γ), IFN-α, TNF-α, and interleukin-1β (IL-1β), and anti-inflammatory cytokines, IL-4, IL-10, and transforming growth factor-β1 (TGF-β1), were studied by ELISA.ResultsIn HPV-induced cervix precancerous diseases, we identified low-avidity IgG antibodies to HSV serum of 20 patients; in the serum of 17 patients, we identified average-avidity antibodies, and high-avidity antibodies were found in 2 patients only. In 14 HPV-negative patients, we found low-avidity IgG antibodies to HSV; in 10 patients, medium avidity. Patients with low-avidity IgG antibodies to herpes virus showed high and medium oncogenic risk HPV types and a decrease of IFN-γ compared to patients with medium-avidity IgG antibodies. Production of IFN-γ was suppressed also in HPV-negative patients with cervical precancers, but we found low- and medium-avidity IgG antibodies to herpes virus. In patients with low-avidity antibodies, we observed increased level of IL-10. Level of IFN-α, IL-1β, IL-2, and IL-4 did not change in patients of all groups, but TGF-β1 increased.ConclusionsIn HPV-positive patients, those with low-avidity IgG antibodies to HSV had immunosuppression, confirmed by increased TGF-β1 and violation of IFN-γ production. Therefore, in pro- and anti-inflammatory cytokines and IgG antibodies to HSV, their avidity is an important diagnostic biomarker of HPV-induced precancerous cervical diseases. Low-avidity IgG antibodies may be an indication for treatment with immunomodulators and antiviral drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%