New Bioartificial Polymeric Material: Poly(ethylene glycol) Cross-Linked with Albumin. I. Synthesis and Swelling Properties

D'Urso, Edith Marie; Fortier, Guy

doi:10.1177/088391159400900402

Cited by 22 publications

(9 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The details of the hydrogel synthesis have been published elsewhere. 2,[18][19][20][21] In brief, hydrogel formation is achieved via a condensation reaction between the amino groups of protein and carbonate-activated PEG with subsequent release of p-nitrophenol molecules. As a result of this reaction, stable urethane link-ages between a biopolymer and PEG are formed.…”

Section: Synthesis Of Hydrogelsmentioning

confidence: 99%

“…2 The protein component of the hydrogel contributes to the ''tissue-like'' properties, whereas the bioinert polymer chains of PEG are responsible for the hydrophilicity of the material. This hydrogel contains up to 96% of water 2,18,19 and is presently used as a moist wound dressing and transdermal drug delivery device. Recent studies demonstrated that the PEG-protein hydrogel is inflammatory inert and accelerates wound closure in vivo.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical and microstructural properties of hybrid poly(ethylene glycol)–soy protein hydrogels for wound dressing applications

Snyders

Shingel²,

Zabeida

et al. 2007

J Biomedical Materials Res

View full text Add to dashboard Cite

Biomimetic hydrogel made of poly(ethylene glycol) and soy protein with a water content of 96% has been developed for moist wound dressing applications. In this study, such hybrid hydrogels were investigated by both tensile and unconfined compression measurements in order to understand the relationships between structural parameters of the network, its mechanical properties and protein absorption in vitro. Elastic moduli were found to vary from 1 to 17 kPa depending on the composition, while the Poisson's ratio (approximately 0.18) and deformation at break (approximately 300%) showed no dependence on this parameter. Further calculations yielded the crosslinking concentration, the average molecular weight between crosslinks (M(C)) and the mesh size. The results show that reactions between PEG and protein create polymeric chains comprising molecules of PEG and protein fragments between crosslinks. M(C) is three times higher than that expected for a "theoretical network." On the basis of this data, we propose a model for the 3D network of the hydrogel, which is found to be useful for understanding drug release properties and biomedical potential of the studied material.

show abstract

Section: Synthesis Of Hydrogelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanical and microstructural properties of hybrid poly(ethylene glycol)–soy protein hydrogels for wound dressing applications

Snyders

Shingel²,

Zabeida

et al. 2007

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…">Second, using albumin mixed with PEG‐6000 in a 6:1 ratio. A viscous gel‐like solution was prepared by dissolving 300 mg of albumin and 1 mg of UCNP in 1 ml of distilled water (30% w/v), to which 50 mg of activated PEG powder was added [18].…”

Section: Methodsmentioning

confidence: 99%

Albumin‐PEG‐Based Biomaterial for Laser‐Tissue Soldering and Its Real‐Time Monitoring With Swept‐Source Optical Coherence Tomography

et al. 2021

View full text Add to dashboard Cite

Background and Objectives The study presents a noninvasive, real‐time monitoring technique for the cross‐sectional imaging of the laser‐tissue soldering process with a swept‐source optical coherence tomography (SSOCT) system. The study also aims at performing a comparative study of the laser‐tissue soldering (LTS) process using optimized compositions of albumin as solder biomaterials. Study Design/Materials and Methods The experimental study was conducted both ex vivo and in vivo to assess the superiority of the LTS process over conventional methods using a noninvasive imaging tool. In our attempt to combine the two techniques into one diagnostic tool, we have used the SSOCT system for a thoroughgoing investigation of the process in real‐time. Laser‐assisted tissue soldering was performed using a pulsed near‐infrared (NIR) laser with a central wavelength of 980 nm, an output power of 5 W, and beam diameter (1/e 2) of 6 mm. Here, the SSOCT system has been utilized to observe and analyze the transitions taking place in real‐time without disrupting the process. For the comparative study, we have used serum albumin in a 70% w/v concentration and albumin‐PEG conjugate in a 6:1 ratio as soldering materials. Different stages of the laser interaction process were monitored with OCT B‐scans of the incision area. Also, the basis of biomaterial‐tissue interaction was studied with the help of Fourier‐transform infrared spectroscopy (FTIR) analysis of the soldering materials. Results FTIR spectrum alludes to the fact that the intertwining of the soldering biomaterial with tissue collagen creates adhesion. Biomaterial serum albumin with 70% w/v concentration as soldering material demonstrates complete sealing of tissue at the incision with 3 minutes of laser irradiation. SSOCT B‐scans have been useful in imaging the incision noninvasively at different stages. Conclusion Both ex vivo and in vivo demonstration of the LTS process were presented with a clinical resemblance. OCT can be of great value to determine the wound contraction in case of incisional wounds or sealed wounds produced by the LTS procedure. Also, volumetric measurements of percentage reduction in wound area can be done with OCT. SSOCT system can be a potential imaging modality for real‐time noninvasive imaging of surgical procedures like LTS. Lasers Surg. Med. © 2021 Wiley Periodicals LLC

show abstract

“…Activation of PEG with p-nitrophenyl-chloroformate was described previously and yielded di(p-nitrophenylcarbonate)PEG (PEGa) (5). To a solution of BSA (50 mg/ml) in 400 mM sodium borate buffer was added the desired amount of PEGa to achieve a molar ratio of OH/NH2 of 1.4 (6). The mixed and degassed solution was poured between two glass plates mounted in a MiniProtean II electrophoresis gel maker and allowed to polymerize.…”

Section: Methodsmentioning

confidence: 99%

New Bioartificial Hydrogels: Characterization and Physical Properties

Gayet

Fortier

1996

ACS Symposium Series

View full text Add to dashboard Cite

Physical properties of a bioartificial hydrogel prepared by cross-linking activated poly(ethylene glycol) of molecular mass of 8000 with bovine serum albumin (BSA) are described. This hydrogel shows a great swelling capacity and a high equilibrium water content (EWC > 95%). The volume expansion factor of a BSA-PEG 8000 hydrogel during swelling was a function of its initial thickness. This hydrogel shows a high elasticity since it was reversibly deformable until a level of compression of 60% and it broke only when this level reached 80%. DSC heating scans demonstrated that differentiation of two kinds of water in the hydrogel is possible : the free (bulk) and the bound water. There was evidence that bound water is in the form of a trihydrate complex with ethylene oxide units of the PEG in the network. This hydrogel was suitable for controlled drug release systems, as demonstrated with release experiments.Hydrogels have attracted significant attention during the last decade for their potential use in the biomedical field (1-2). They are a hydrophilic polymeric network which is glassy in the dehydrated state and which swells in the presence of water to form an elastic gel. Whether they are of natural or synthetic origin, they share a high water content, a permeability to small (even large) molecules, a cross-linked structure and a potentially good biocompatibility. Biomedical applications of hydrogels are extending into areas such as materials for blood contact, contact lenses, artificial tendons, controlled release devices, bioadhesive materials, and so forth. Since synthetic polymers are available with a wide variety of compositions, properties and forms, and exhibit good mechanical behaviour, they often lack biocompatibility in living systems, whereas natural polymers have the opposite properties. New bioartificial polymeric materials which combine properties of synthetic and natural polymers may overcome the deficiencies of synthetic polymers and enhance the mechanical properties of biopolymers, leading to a better composite material (3). In agreement with this point of view, we have recendy introduced a new family of bioartificial hydrogels prepared by cross-linking activated poly(ethylene glycol) (PEG) of various molecular masses with bovine serum albumin (BSA) (4). We present herein the characterization and some physical properties of a hydrogel made with PEG of molecular mass of 8000 (BSA-PEG 8000).

show abstract

New Bioartificial Polymeric Material: Poly(ethylene glycol) Cross-Linked with Albumin. I. Synthesis and Swelling Properties

Cited by 22 publications

References 29 publications

Mechanical and microstructural properties of hybrid poly(ethylene glycol)–soy protein hydrogels for wound dressing applications

Mechanical and microstructural properties of hybrid poly(ethylene glycol)–soy protein hydrogels for wound dressing applications

Albumin‐PEG‐Based Biomaterial for Laser‐Tissue Soldering and Its Real‐Time Monitoring With Swept‐Source Optical Coherence Tomography

New Bioartificial Hydrogels: Characterization and Physical Properties

Contact Info

Product

Resources

About