New B-cell CD molecules

Matesanz-Isabel, Jessica; Sintes, Jordi; Llinàs, Laia; Salort, José de; Lázaro, Adriana; Engel, Pablo

doi:10.1016/j.imlet.2010.09.019

Cited by 64 publications

(47 citation statements)

References 85 publications

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“…After washing, F99 mAb was added at 50 ng/ml and bound mAb was detected with alkaline phosphatase-labeled goat anti-mouse IgG. In flow cytometry analysis, 293T cells transfected with pcDNA3-based expression plasmids encoding FCRL1-5 were incubated with 1 mg/ml FCRL5 F99 or the following reference anti-FCRL1-5 mouse Abs: FCRL1 (clone E3, IgG1), FCRL2 (clone B24, IgG2a), FCRL3 (clone H5, IgG2b), FCRL4 (clone A1, IgG2a), and FCRL5 (clone F56, IgG1) (30,31). Cell-bound mAb was detected by PE-conjugated goat antimouse IgG F(ab9) 2 (Jackson ImmunoResearch Laboratories) using BD Accuri C6 (BD Biosciences).…”

Section: Reactivity Of Anti-fcrl5 F99 Mabmentioning

confidence: 99%

Fc Receptor–like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue–like Memory B Cells

Borrego

Nagata

et al. 2016

The Journal of Immunology

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Fc receptor–like (FCRL) 5 is a novel IgG binding protein expressed on B cells, with the capacity to regulate Ag receptor signaling. We assessed FCRL5 expression on circulating B cells from healthy donors and found that FCRL5+ cells are most enriched among atypical CD21−/lo/CD27− tissue-like memory (TLM) B cells, which are abnormally expanded in several autoimmune and infectious diseases. Using multicolor flow cytometry, FCRL5+ TLM cells were found to express more CD11c and several inhibitory receptors than did the FCRL5− TLM subset. The homing receptor profiles of the two TLM subsets shared features consistent with migration away from lymphoid tissues, but they also displayed distinct differences. Analysis of IgH V regions in single cells indicated that although both subsets are diverse, the FCRL5+ subset accumulated significantly more somatic mutations. Furthermore, the FCRL5+ subset had more switched isotype expression and more extensive proliferative history. Microarray analysis and quantitative RT-PCR demonstrated that the two TLM subsets possess distinct gene expression profiles, characterized by markedly different CD11c, SOX5, T-bet, and RTN4R expression, as well as differences in expression of inhibitory receptors. Functional analysis revealed that the FCRL5+ TLM subset responds poorly to multiple stimuli compared with the FCRL5− subset, as reflected by reduced calcium mobilization and blunted cell proliferation. We propose that the FCRL5+ TLM subset, but not the FCRL5− TLM subset, underwent Ag-driven development and is severely dysfunctional. The present study elucidates the heterogeneity of TLM B cells and provides the basis to dissect their roles in the pathogenesis of inflammatory and infectious diseases.

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Section: Reactivity Of Anti-fcrl5 F99 Mabmentioning

confidence: 99%

Fc Receptor–like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue–like Memory B Cells

Borrego

Nagata

et al. 2016

The Journal of Immunology

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“…Deficiencies seem to affect the Th2 response to both T-dependent and T-independent antigens and the marker has been suggested to be a potential therapeutic target for treating inflammatory and autoimmune diseases and malignancies. 49,50 Shown here are the genes on which SNPs most strongly associated with inhibitor development in the Combined Cohort and the discordant pairs are located.…”

Section: Pcgf2 Rs2879097mentioning

confidence: 99%

The polygenic nature of inhibitors in hemophilia A: results from the Hemophilia Inhibitor Genetics Study (HIGS) Combined Cohort

Astermark

Donfield

Gomperts

et al. 2013

Blood

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“…Fcα/μR is an Fc receptor for IgA and IgM described in mice and humans (Shibuya et al, 2000;Sakamoto et al, 2001;Nakahara et al, 2003;Matesanz-Isabel et al, 2010). The mouse receptor is encoded by a single gene located on chromosome 1 (1F), coding for a type I transmembrane protein with a 32-amino-acid leader sequence, a 423-amino-acid EC domain, a 20-amino-acid transmembrane domain, and a 60-amino-acid cytoplasmic region (Shibuya et al, 2000;Sakamoto et al, 2001;Shimizu et al, 2001).…”

Section: Fcα/μrmentioning

confidence: 99%

“…A human homolog of Fcα/μR has been described (hFcα/μR, CD351) (Shibuya et al, 2000;Sakamoto et al, 2001;Matesanz-Isabel et al, 2010) that shares 49% amino acid identity with the murine receptor. The gene is located at chromosome 1q32.3 near genes that encode pIgR, FcεRI, and FcγRI .…”

Section: Fcα/μrmentioning

confidence: 99%