New attachment formation as the result of controlled tissue regeneration

Abstract

The present study was designed to examine whether new attachment forms on root surfaces previously exposed to plaque by preventing the oral epithelium and the gingival connective tissue from participating in the process of healing following treatment. 4 roots in each of 3 monkeys were used as test units while the roots of contralateral teeth served as controls. A surgical procedure was first used to expose the coronal half of the buccal root surfaces. Plaque was allowed to accumulate on the exposed surfaces for a period of 6 months. Subsequently, soft tissue flaps were raised and the root surfaces were carefully scaled and planed. The crowns of the test and control teeth were resected and the mucosal flaps were repositioned and sutured in such a way that the roots were properly covered. Immediately prior to suturing, membranes (Millipore filter or Gore-tex membrane) were placed over the denuded root surfaces of the test teeth in order to prevent granulation tissue from the soft tissue flaps from reaching the roots during healing. The monkeys were sacrificed 3 months later. The jaws were removed and histological sections of test and control roots including their periodontal tissues were produced. New cementum with inserting collagen fibers was observed on the previously exposed surfaces of both test and control roots. However, the test surfaces exhibited considerably more new attachment than the control surfaces, indicating that the placement of the membrane favoured repopulation of the wound area adjacent to the roots by cells originating from the periodontal ligament.

Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery

“…Indeed, the cementum regeneration rate was more than 70% in all groups in this study ( Table 2). The cementum regeneration rate has been generally used as an index for cementum regeneration in previous studies (13,15,20,22,23). However, as the detached cementum is also counted, this index does not strictly reflect the index of the regeneration of cementum attached strongly to the dentin.…”

“…To date, many investigators have applied various kinds of clinical treatments to accelerate periodontal tissue regeneration (13,14,20,22). However, in these studies, the regenerated cementum frequently detached from the root surface (13,14,20,22). Some reports suggest that the detachment occurs at a collagen fiber-poor layer between the regenerated cementum and the root surface (4,16,17).…”

“…For this purpose, cementum regeneration on exposed roots and periodontal ligament regeneration with functional arrangement of collagen fibers are necessary, together with the regeneration of supporting alveolar bone. To date, many investigators have applied various kinds of clinical treatments to accelerate periodontal tissue regeneration (13,14,20,22). However, in these studies, the regenerated cementum frequently detached from the root surface (13,14,20,22).…”

Comparison of Th1/Th2 cytokine profiles of initial wound healing of rats induced by PDCM and e‐PTFE

“…1 However, e-PTFE is non biodegradable, requires secondary surgery for its removal, and cannot be adapted on the developmental groove on the furcal entrances of molars and interdental lesions. 2 The exposed e-PTFE (occurrence rate: 70 -80%) are prone to contamination with oral bacteria, which may lead to infection of the generated site.…”

Estudo da regeneração óssea guiada em mandíbula de ratos idosos

“…Na área da periodontia, a técnica foi inicialmente demonstrada em um estudo experimental que objetivou a regeneração do periodonto de inserção (Nyman et al, 1982). Outros estudos em animais (Gottlow et al, 1984;Karring et al, 1985) e em humanos (Gottlow et al,1986) comprovaram que ocorre a regeneração do periodonto de inserção, através da técnica de ROT.…”