New assay to diagnose and differentiate between Mycobacterium tuberculosis complex and nontuberculous mycobacteria

Ustinova, Vera V.; Smirnova, Tatiana G.; Сочивко, Д. Г.; Varlamov, D. A.; Ларионова, Е. Е.; Andreevskaya, Sofya; Andrievskaya, I.; Ev, Kiseleva; Черноусова, Л. Н.; Ergeshov, Atadzhan

doi:10.1016/j.tube.2018.10.004

Cited by 19 publications

(5 citation statements)

References 32 publications

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“…Various molecular diagnostic techniques for NTM, including conventional PCR, real-time PCR, PCR restriction fragment length polymorphism analysis, oligonucleotide array and sequencing, have been developed and evaluated (Jung et al, 2016). However, multiple studies have confirmed that although the PCR method has a high specificity, its sensitivity is not high enough (35.3-73.1%) regardless of the type of specimen and PCR method (Kim et al, 2015;Ustinova et al, 2019). Gene sequencing can identify the level of NTM subspecies, but its wide use in clinical practice is difficult due to the laboratory requirements, time-consuming process, difficulty, and cost (de Zwaan et al, 2014;van Ingen, 2015).…”

Section: Introductionmentioning

confidence: 99%

Application of acid-fast staining combined with GeneXpert MTB/RIF in the diagnosis of non-tuberculous mycobacteria pulmonary disease

Kong

Xie

Liu

et al. 2021

International Journal of Infectious Diseases

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Section: Introductionmentioning

confidence: 99%

Application of acid-fast staining combined with GeneXpert MTB/RIF in the diagnosis of non-tuberculous mycobacteria pulmonary disease

Kong

Xie

Liu

et al. 2021

International Journal of Infectious Diseases

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“…In addition, NTM was detected in 7 of 100 AFB smear-positive specimens. The coexistence of MTB and NTM has been reported in several studies ( 20 – 22 ), but most of them were case reports. This shows the importance of diagnosing the co-infection of MTB and NTM in patients with active tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria coexisting in patients with pulmonary tuberculosis by single-tube multiplex PCR

Heidari,

Dehbidi,

Farhadi

et al. 2023

IJM

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Background and Objectives: In clinical diagnostics, molecular methods are used to detect Mycobacterium tuberculosis bacilli (MTB) and to distinguish them from non-tuberculous mycobacteria (NTM). They are also used to make the right treatment decision for the patient as soon as possible. The aim of this study was to establish a rapid and novel multiplex PCR (mPCR) assay for the detection and differentiation of MTB and NTM in a single tube. Materials and Methods: 100 sputum samples positive for acid-fast bacilli (AFB) were included in this study. Mycobacterial culture, biochemical tests, and antibiotic susceptibility testing were performed on samples. After alkaline decontamination, total DNA was extracted from the samples. A primer pair targeting the rpoB gene, encoding the beta-subunit of RNA poly- merase, was used to detect MTB and NTM, amplifying a 235-bp fragment of MTB and a 136-bp sequence of NTM. A pair of primers targeting a 190-bp fragment of the IS6110 region of MTB was also used to confirm the results. The sensitivity and specificity of the mPCR assay were evaluated using DNA extracted from standard strains. The amplified products were then analyzed by conventional agarose gel electrophoresis. Results: Of 100 AFB smear-positive sputum samples, 92 MTB DNA, 7 NTM DNA, and one mixed-infection sample were identified in a single tube using mPCR assay. There was no correlation between the AFB degree of smear positivity and PCR results. Of seven NTM isolates, 6 (86%) were resistant to rifampin, isoniazid, and ethambutol, the three first-line anti-tuber- culosis drugs. Conclusion: A single-tube mPCR assay based on the rpoB gene provides a rapid and reliable means of detecting and differentiating MTB and NTM in sputum specimens.

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“…Актуальность вопроса одновременного выявления в биологическом материале нетуберкулезных микобактерий и микобактериального туберкулезного комплекса [11] представляет большой интерес [11]. При анализе известных информационных источников, включая PubMed, нами не обнаружено схожих наблюдений у одного больного сочетанной инфекции Mycobacterium avium и tuberculosis.…”

Section: Abstract: Skin Mycobacteriosis Mycobacterium Avium Pulmonaunclassified

A clinical observation of a Mycobacterium avium skin lesion in a patient with pulmonary tuberculosis

Митрушкина

Smerdin

Stavitskaya

et al. 2020

Vestnik dermatologii i venerologii

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Микобактерии туберкулеза и нетуберкулезные микобактерии (НТМБ) клинически различны. Быстрое выявление и дифференциация M. tuberculosis complex (MTBC) и НТМБ имеет решающее значение для инфекционного контроля. Ввиду высокой их распространенности вероятность коинфекции достаточно высока, но сведения о распространенности и встречаемости подобных сочетаний крайне малочисленны. Проведено изучение клинической ситуации и показано формирование терапевтической стратегии в случае наличия у больного сочетанной инфекции Mycobacterium avium и tuberculosis. Ключевые слова: микобактериоз кожи, Mycobacterium avium, туберкулез органов дыхания Конфликт интересов: авторы заявляют об отсутствии потенциального конфликта интересов, требующего раскрытия в данной статье. Финансирование: клиническое наблюдение было проведено в стационарном отделении ГБУЗ МО «Московский областной клинический противотуберкулезный диспансер». Благодарности: авторы выражают благодарность за подготовку материалов статьи Филиппову Павлу Николаевичу, врачу-бактериологу высшей категории, ГБУЗ МО «Московский областной клинический противотуберкулезный диспансер»; Калугиной Светлане Михайловне, врачу-патологоанатому, заведующей лабораторным отделением, ФГКУ «1586 ВКГ»; Поплавскому Александру Валерьевичу, врачу-общему хирургу высшей категории, ГБУЗ МО «Московский областной клинический противотуберкулезный диспансер»; Никитиной Екатерине Сергеевне, врачу-фтизиатру высшей категории, заведующей отделением, ГБУЗ МО «Московский областной клинический противотуберкулезный диспансер».

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New assay to diagnose and differentiate between Mycobacterium tuberculosis complex and nontuberculous mycobacteria

Cited by 19 publications

References 32 publications

Application of acid-fast staining combined with GeneXpert MTB/RIF in the diagnosis of non-tuberculous mycobacteria pulmonary disease

Application of acid-fast staining combined with GeneXpert MTB/RIF in the diagnosis of non-tuberculous mycobacteria pulmonary disease

Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria coexisting in patients with pulmonary tuberculosis by single-tube multiplex PCR

A clinical observation of a Mycobacterium avium skin lesion in a patient with pulmonary tuberculosis

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