New Aspects of Virus Elimination in Fruit Trees

Knapp, E.; Hanzer, V.; Weiß, H.; Machado, Artur da Câmara; Weiss, Batia; Wang, Q.; Katinger, Hermann; Machado, M. Laimer da Câmara

doi:10.17660/actahortic.1995.386.56

Cited by 37 publications

(21 citation statements)

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“…However, our results showed that a relatively lower concentration (1 mg/L) and shorter period (4 weeks) of treatment could also be adequate for in vitro elimination of PPV from apricot. This result could be due to the fact that the efficiency of ribavirin in elimination of plant viruses depends on its concentration and the genotype of the both host plant and virus (Knapp et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

Şeker¹,

Süzerer²,

Elibüyük³

et al. 2015

IJAB

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Plum pox potyvirus (PPV) is the most serious viral disease of stone fruits of Prunus sp. and elimination of the virus could be achieved by using in vitro-applied techniques. Thus, the potential of usage of thermotherapy, chemotherapy and cryotherapy techniques was assessed by in vitro treatment of infected apricot shoot tips. Survival was declined with prolonged application of thermotherapy whereas promising results were scored after chemotherapy and cryotherapy. The highest survival (100%) of the shoot tips was achieved with quercetin containing medium while relatively lower (80%) survival was scored with ribavirin. In addition, inclusion of the 8-azaguanine had found to be phytotoxic as no survival was achieved after treatment. In the case of cryotherapy, two-step freezing technique was applied and 10% of survival was recorded in cryopreserved apricot shoot tips. RT-PCR analysis showed that PPV was eliminated in the survived ones both after chemotherapy and cryotherapy, which revealed that those techniques could be used for in vitro elimination of PPV from infected apricot as well as other stone fruits.

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Section: Discussionmentioning

confidence: 99%

In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

Şeker¹,

Süzerer²,

Elibüyük³

et al. 2015

IJAB

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“…This combination has been shown to be effective [12] [13] although the percentage of recovery of viable plants free of virus was relatively low. Thermotherapy is most frequently performed at 36˚C -38˚C for 21 -35 days [14]. However, maintaining high temperatures for long periods of time has a negative effect on the survival and growth of meristems even though it is more effective in achieving high rates of virus-free plants [15].…”

Section: Introductionmentioning

confidence: 99%

Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees

Lizárraga¹,

Ascasíbar²,

Fernández-González³

2017

AJPS

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Heat therapy followed by the isolation and in vitro culture of apical meristems is a suitable procedure for virus eradication. However, the period of heat treatment is usually long (28 -50 days) and the yield of viable plants free of viruses after treatment is often low (<50%). Here, we describe an alternative method to obtain virus-free plants. We used traditional Galician cultivars, six apple trees and two pear trees, infected with Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). We combined heat therapy of in vitro shoots using a temperature gradient from 25˚C to 40˚C increasing 1˚C per day for a shorter period of time (18 days) with the posterior isolation and culture of apical meristems. All DNA samples analyzed, obtained from plants developed from meristems, were 100% free of ApMV and almost 90% free of ACLSV. With this in vitro procedure combined we obtained a good yield of tested plants free of viruses. Our method is fast and effective and it could be also useful to eradicate these and other viruses in other fruit trees.

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“…Some authors also reported that especially mixed infections of more than one virus are more diffi cult to eliminate (Knapp et al 1995;Da Câmara Machado et al 1998;Wang et al 2006;Paprstein et al 2008).…”

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confidence: 99%

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

Paprštein

Sedlák

Svobodová

et al. 2013

Hort. Sci. (Prague)

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Parštein F., Sedlák J., Svobodová L., Polák J., Gadiou S., 2013. Results of in vitro chemotherapy of apple cultivar Fragrance -Short communication. Hort. Sci. (Prague),.Th e eff ect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. Th e results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial fi eld-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with welldeveloped leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the fi rst chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. Th e success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.

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New Aspects of Virus Elimination in Fruit Trees

Cited by 37 publications

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In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

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New Aspects of Virus Elimination in Fruit Trees

Cited by 37 publications

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In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

In Vitro Elimination of PPV from Infected Apricot Shoot Tips via Chemotherapy and Cryotherapy

Fast and Effective Thermotherapy Treatment for &lt;i&gt;In Vitro&lt;/i&gt; Virus Eradication in Apple and Pear Trees

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

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Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees