New aspects of the glucose activation of the H+-ATPase in the yeast Saccharomyces cerevisiae

Abstract

The glucose-induced activation of plasma membrane ATPase from Saccharomyces cerevisiae was first described by Serrano in 1983. Many aspects of this signal transduction pathway are still obscure. In this paper, evidence is presented for the involvement of Snf3p as the glucose sensor related to this activation process. It is shown that, in addition to glucose detection by Snf3p, sugar transport is also necessary for activation of the ATPase. The participation of the G protein, Gpa2p, in transducing the internal signal (phosphorylated sugars) is also demonstrated. Moreover, the involvement of protein kinase C in the regulation of ATPase activity is confirmed. Finally, a model pathway is presented for sensing and transmission of the glucose activation signal of the yeast H M -ATPase.

Genome-wide identification of the Fermentome; genes required for successful and timely completion of wine-like fermentation by Saccharomyces cerevisiae

“…Pma1p activation is dependent on a complex interaction between glucose sensors such as Snf3p and Rgt2p, and the Gpr1p/Gpa2p receptor/G protein-coupled (GPCR) complex [57], protein kinase C (Pkc1p) [57], phospholipase C (Plc1p) [58], the proteosome, and interaction with tubulin (as reviewed in [54]). Several studies conducted on glucose starved cells, using specific inhibitors of Plc1 (3-nitrocourmarin) [59, 60] and Pma1p (carbonyl cyanide m-chlorophenylhydrazone; CCCP) [61] in conjunction with glucose additions (up to 100 mM or 18 g L −1 ) have demonstrated the activation of Pma1p to be mediated by Plc1p and the GPCR complex.…”

The plasma membrane H+-ATPase is related to the development of salicylic acid-induced thermotolerance in pea leaves

“…-ATPase activity in yeast cells (Coccetti et al 1998;Souza et al 2001). Therefore, clarifying firstly, the interaction of the PM H ?…”

“…-ATPase increased remarkably, and then stimulate the amplification of signals, for example, Ca 2? (Kinoshita et al 1995), lipid (Kasamo 2003), the phosphorylated sugars (Souza et al 2001) etc., and finally represent in maintaining the integrity of plasma membrane, in result, evaluate the thermotolerance of plant to heat stress.…”

From bit to it: How a complex metabolic network transforms information into living matter

“…One example is the sensing of glucose and protons (H + ), where in yeast the glucose sensor Snf3p is known to activate the proton pumping plasma membrane ATPase [47]. …”