New artificial connective matrix-like structure made of elastin solubilized peptides and collagens: elaboration, biochemical and structural properties

Lefèbvre, F.; Gorecki, Simon; Bareille, Reine; Amédée, Joëlle; Bordenave, Laurence; Rabaud, M.

doi:10.1016/0142-9612(92)90091-2

Cited by 45 publications

(20 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell biological effect chapter 1 elastin as a biomaterial for tissue engineering may be modulated by the amount of solubilised elastin in the scaffold and the extent of crosslinking. Materials based upon α-elastin or elastin fi bres with type I and III collagen can be prepared [149,150], in combination with glycosaminoglycans [151] or calcium phosphate [152]. Elastin preparations combined with fi brin have also been prepared [153].…”

Section: Figure 5 Transmission Electron Micrographs (A C) and Scannmentioning

confidence: 99%

Elastin as a biomaterial for tissue engineering

et al. 2007

View full text Add to dashboard Cite

Section: Figure 5 Transmission Electron Micrographs (A C) and Scannmentioning

confidence: 99%

Elastin as a biomaterial for tissue engineering

et al. 2007

View full text Add to dashboard Cite

“…Modifications of these matrices have allowed various biomaterials to be obtained [2,31. The association of the matrices with calcium phosphate should lead to interesting bone substitutes presenting acceptable mechanical properties and being biodegradable.…”

Section: Introductionmentioning

confidence: 99%

XPS Study of elastin-solubilized peptides binding onto apatite in orthopaedic biomaterials

Lebugle¹,

Rovira

Rabaud

et al. 1996

J Mater Sci: Mater Med

Self Cite

View full text Add to dashboard Cite

The adsorption behaviour of elastin-solubilized peptides (ESP) on hydroxyapatite (HA) was studied by X-ray photoelectron spectroscopy (XPS). The analysis of the data indicated a modification of both the apatitic surface and the peptide moieties. The aliphatic and aromatic carbon peak increased with respect to the hydrophilic carbon peak indicating a preferential orientation of the peptide molecules interacting essentially with the apatite surface by their hydrophilic groups. A deprotonation of the amine groups of adsorbed peptides was also observed, revealing a possibly chemical interaction with the mineral surface. Simultaneously a decrease of the Ca/P ratio of the mineral surface occurred. The orientation of ESP adsorbed on the mineral surface was related to the modification of their reactivity with collagens I + III in the process of formation of composite biomaterials for orthopaedic applications. IntroductionA new artificial connective matrix based on the polar association between elastin peptides-ESP and type I +III collagens has recently been developed by one of the authors [1]. Modifications of these matrices have allowed various biomaterials to be obtained [2, 31. The association of the matrices with calcium phosphate should lead to interesting bone substitutes presenting acceptable mechanical properties and being biodegradable. Moreover the presence of apatite gives hope for osteoconductive properties for such new bone substitutes. However, the various components of the material have to be linked together. To do this, one solution is to adsorb ESP onto apatite (the adsorption properties of proteins onto calcium phosphate are well known), and then to perform the reaction with collagen. Previous studies have shown that ESP are adsorbed irreversibly onto apatite [-4"].A surface analysis method has been used to study the mechanisms which take place during the adsorption of ESP onto apatite. The apatite and ESP were studied before and after adsorption onto apatite. The study concerned both the quantitative aspect of the adsorption and the change in orientation which could result.

show abstract

“…This substance has been markedly improved by adding connective or adhesive proteins and proteoglycans to yield artificial extracellular matrices: with heparan sulphate proteoglycan, it resembles more closely the natural subendothelial extracellular matrix [6,7].…”

Section: Introductionmentioning

confidence: 99%

Preliminary report on a new composite material made of calcium phosphate, elastin peptides and collagens

Rovira

Bareille

López

et al. 1993

J Mater Sci: Mater Med

Self Cite

View full text Add to dashboard Cite

With a view to elaborating a bioactive bone substitute, the association of an artificial extracellular matrix, basically constituted of elastin-solubilized peptides (ESP) and type I + III collagens, with different types of calcium phosphates, was investigated. This paper describes the selective adsorption of ESP on some calcium phosphate samples, and the further association of the adsorbed peptides with type I + III collagens. A preliminary study of the material cytotoxicity was carried out, investigating the behaviour of human osteoblast cells in contact with the yielded composite material.

show abstract

New artificial connective matrix-like structure made of elastin solubilized peptides and collagens: elaboration, biochemical and structural properties

Cited by 45 publications

References 7 publications

Elastin as a biomaterial for tissue engineering

Elastin as a biomaterial for tissue engineering

XPS Study of elastin-solubilized peptides binding onto apatite in orthopaedic biomaterials

Preliminary report on a new composite material made of calcium phosphate, elastin peptides and collagens

Contact Info

Product

Resources

About