Abstract:Serial sections (each from 150 to 200 μm thick) of porcine molar tooth germs within their bony crypts, rodent incisor teeth (in situ), and human extracted teeth were cut with a thin rotating diamond-impregnated disc, without prior embedding. Some specimens were cut unfixed, at room temperature (21°C) or frozen (-70°C), some in fixative, and others cut after fixation. A variety of routine fixatives has been tried, and in general the preservation of hard/soft tissue interfaces is best achieved when fixation prec… Show more
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