New Approaches to Understanding Bacterial Histidine Kinase Activity and Inhibition

Wilke, Kaelyn E.; Carlson, Erin E.

doi:10.1002/9783527683031.ch10

Cited by 2 publications

(2 citation statements)

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“…), antibiotic resistance, and communication, making them an attractive target for antibiotic development. 11,12 There are up to 150 different TCSs per organism, responding to various stimuli. 7 Therefore, methods to globally study TCSs are critical to understanding their activity, function, and the stimuli which trigger them, as most remain unknown to date.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Histidine phosphorylation plays crucial roles in prokaryotes in two-component signal transduction systems (TCSs), phosphorelays, and in metabolism by helping the organisms to respond to their continuously changing environment and in eukaryotes in various cellular mechanisms such as phagocytosis and cell cycle events. ,− The TCSs are particularly of interest, as they are involved in numerous bacterial processes, including growth and cell maintenance, but also bacterial virulence (biofilm formation, toxin production, etc. ), antibiotic resistance, and communication, making them an attractive target for antibiotic development. , There are up to 150 different TCSs per organism, responding to various stimuli . Therefore, methods to globally study TCSs are critical to understanding their activity, function, and the stimuli which trigger them, as most remain unknown to date …”

Section: Introductionmentioning

confidence: 99%

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Mechanistic Studies of Bioorthogonal ATP Analogues for Assessment of Histidine Kinase Autophosphorylation

et al. 2020

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Phosphorylation is an essential protein modification and is most commonly associated with hydroxyl-containing amino acids via an adenosine triphosphate (ATP) substrate. The last decades have brought greater appreciation to the roles that phosphorylation of myriad amino acids plays in biological signaling, metabolism, and gene transcription. Histidine phosphorylation occurs in both eukaryotes and prokaryotes but has been shown to dominate signaling networks in the latter due to its role in microbial two-component systems. Methods to investigate histidine phosphorylation have lagged behind those to study serine, threonine, and tyrosine modifications due to its inherent instability and the historical view that this protein modification was rare. An important strategy to overcome the reactivity of phosphohistidine is the development of substrate-based probes with altered chemical properties that improve modification longevity but that do not suffer from poor recognition or transfer by the protein. Here, we present combined experimental and computational studies to better understand the molecular requirements for efficient histidine phosphorylation by comparison of the native kinase substrate, ATP, and alkylated ATP derivatives. While recognition of the substrates by the histidine kinases is an important parameter for the formation of phosphohistidine derivatives, reaction sterics also affect the outcome. In addition, we found that stability of the resulting phosphohistidine moieties correlates with the stability of their hydrolysis products, specifically with their free energy in solution. Interestingly, alkylation dramatically affects the stability of the phosphohistidine derivatives at very acidic pH values. These results provide critical mechanistic insights into histidine phosphorylation and will facilitate the design of future probes to study enzymatic histidine phosphorylation.

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Section: ■ Introductionmentioning

confidence: 99%