New Approaches to Study Metal-Induced Stress in Plants

Cicarelli, Mariana; Capaldi, Flávia Regina; Carvalho, Rogério Falleiros; Gratão, Priscila Lupino; Azevedo, Ricardo Antunes

doi:10.1007/978-1-4614-0815-4_19

Cited by 3 publications

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“…The most evident events triggered during senescence are oxidative stress and chlorophyll and protein breakdowns (Khanna-Chopra 2012, Qian et al 2012). Thus, an enhancement in the production of reactive oxygen species (ROS) may occur triggering a complex detoxification mechanism capable of preventing uncontrolled oxidation (Vitória et al 2003, Andrade et al 2009, Cia et al 2012a. ROS-scavenging mechanisms include the action of some key enzymes , such as superoxide dismutase (SOD, EC 1.15.1.1), which dismutates superoxide radical to H 2 O 2 , and a range of peroxidases that break down H 2 O 2 , among others catalase (CAT,EC 1.11.1.6) and ascorbate peroxidase (APX,EC 1.11.1.11) (Gratão et al 2005.…”

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confidence: 99%

Leaf senescence in tomato mutants as affected by irradiance and phytohormones

et al. 2013

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We explored the interaction between radiation of different wavelength and jasmonic acid (JA) or brassinosteroids (BR) on leaf senescence-induced oxidative stress. Three approaches were used: 1) jasmonic acid insensitive1-1 (jai1-1) and brassinosteroid-deficient [dumpy (dpy)] mutants were treated with red (R) or far-red (FR) radiation; 2) phytochromedeficient aurea (au) and high pigment-1 (hp-1) (radiation exaggerated response) mutants were treated with methyl jasmonate (MeJA) or epibrassinolide (epiBL); and 3) double mutants au jai1-1 and au dpy were produced. Leaf chlorophyll content, lipid peroxidation, and antioxidant enzyme activities were determined. After senescence induction in detached leaves, we verified that the patterns of chlorophyll degradation of hormonal and photomorphogenic mutants were not significantly different in comparison with original cv. Micro-Tom (MT). Moreover, there was no significant change in lipid peroxidation measured as malondialdehyde (MDA) production, as well as catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) activities in the hormonal mutants. Exogenous BR increased CAT and APX activities in MT, au, and hp-1. As concerns the double mutants, severe reduction in H 2 O 2 production which was not accompanied by changes in MDA content, and CAT and APX activities was observed during senescence in au dpy. The results suggest that JA and BR do not participate in light signaling pathway during leaf senescence-induced oxidative stress.

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