New Approaches to Lymphatic Imaging

Lucarelli, Richard T.; Ogawa, Mikako; Kosaka, Nobuyuki; Türkbey, Barış; Kobayashi, Hisataka; Choyke, Peter L.

doi:10.1089/lrb.2009.0021

Cited by 41 publications

(46 citation statements)

References 33 publications

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“…Figures 6(d), 6(e) and 6(f) show the automatically detected lymphatic vessel images (animations in Visualization 2, Visualization 3, and Visualization 4), and Figs. 6(g), 6(h) and 6(i) show the combined lymphatic and blood vessel images to a depth of 400 µm below skin surface, respectively, from time points C, D and E. The arrows in Figs. 6(d), 6(e) and 6(f) mark vessel patterns common across the scans.…”

Section: Methodsmentioning

confidence: 99%

“…To provide insights into such physiologies and pathologies, techniques have been developed to image the lymphatic vessels, referred to as lymphangiography. These include blue dye staining, fluorescence optical imaging, computed tomography, magnetic resonance imaging, ultrasound imaging, photoacoustic imaging and positron emission tomography [3][4][5][6]. Such approaches typically require the injection of exogenous contrast agents to label the lymphatic vessels [3], which raises issues of cost, patient safety and waiting time for in vivo imaging in humans.…”

Section: Introductionmentioning

confidence: 99%

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In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

Gong

Es'haghian

Harms

et al. 2016

Biomed. Opt. Express

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Abstract:We present an automated, label-free method for lymphangiography of cutaneous lymphatic vessels in humans in vivo using optical coherence tomography (OCT). This method corrects for the variation in OCT signal due to the confocal function and sensitivity fall-off of a spectral-domain OCT system and utilizes a single-scattering model to compensate for Ascan signal attenuation to enable reliable thresholding of lymphatic vessels. A segmentjoining algorithm is then incorporated into the method to mitigate partial-volume effects with small vessels. The lymphatic vessel images are augmented with images of the blood vessel network, acquired from the speckle decorrelation with additional weighting to differentiate blood vessels from the observed high decorrelation in lymphatic vessels. We demonstrate the method with longitudinal scans of human burn scar patients undergoing ablative fractional laser treatment, showing the visualization of the cutaneous lymphatic and blood vessel networks. Dermatol. Symp. Proc. 5(1), 14-19 (2000). 33. C. Blatter, E. F. J. Meijer, A. S. Nam, D. Jones, B. E. Bouma, T. P. Padera, and B. J. Vakoc, "In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography," Sci. Rep. 6, 29035 (2016).

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

Gong

Es'haghian

Harms

et al. 2016

Biomed. Opt. Express

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“…Fluorescent ICG has frequently been used as an alternative to dyes or radioactive gamma probes to guide sentinel lymph node biopsy (58)(59)(60). Fluorescent molecules offer the advantages of ease of use ease of use and low invasiveness compared to methods requiring exposure to radiation (58).…”

Section: Fluorescent Imaging Of the Lymphaticsmentioning

confidence: 99%

Advances in fluorescent-image guided surgery

2016

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“…3 Although SLN mapping tracers such as blue dyes and radioactive colloids have been used in the clinic, they have a few drawbacks. For example, radioactive agents cause radiation damage to the tissues adjacent to the SLN.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence tomographic imaging of sentinel lymph node using near-infrared emitting bioreducible dextran nanogels

Li¹,

Jiang²,

Lin³

et al. 2014

IJN

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Sentinel lymph node (SLN) mapping is a critical procedure for SLN biopsy and its diagnosis as tumor metastasis in clinical practice. However, SLN mapping agents used in the clinic frequently cause side effects and complications in the patients. Here, we report the development of a near-infrared (NIR) emitting polymeric nanogel with hydrodynamic diameter of ~28 nm – which is the optimal size for SLN uptake – for noninvasive fluorescence mapping of SLN in a mouse. This polymeric nanogel was obtained by coupling Cy7, an NIR dye, to the self-assembled nanogel from disulfide-linked dextran-deoxycholic acid conjugate with the dextran of 10 kDa, denoted as Dex–Cy7. Fluorescence imaging analysis showed that Dex–Cy7 nanogels had an enhanced photostability when compared to Cy7 alone. After intradermal injection of Dex–Cy7 nanogel into the front paw of a mouse, the nanogels were able to migrate into the mouse’s axillary lymph node, exhibiting longer retention time and higher fluorescence intensity in the node when compared to Cy7 alone. An immunohistofluorescence assay revealed that the nanogels were localized in the central region of lymph node and that the uptake was largely by the macrophages. In vitro and in vivo toxicity results indicated that the dextran-based nanogels were of low cytotoxicity at a polymer concentration up to 1,000 μg/mL and harmless to normal liver and kidney organs in mice at an intravenous dose of 1.25 mg/kg. The results of this study suggest that NIR-emitting polymeric nanogels based on bioreducible dextran-deoxycholic acid conjugates show high potential as fluorescence nanoprobes for safe and noninvasive SLN mapping.

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New Approaches to Lymphatic Imaging

Cited by 41 publications

References 33 publications

In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

Advances in fluorescent-image guided surgery

Fluorescence tomographic imaging of sentinel lymph node using near-infrared emitting bioreducible dextran nanogels

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