New Approaches to Linear Gradient Elution Used for Optimization in Reversed-Phase Liquid Chromatography

Enhanced selectivity and search speed for method development using one-segment-per-component optimization strategies

“…In reversed-phase liquid chromatography, selectivity can be obtained by varying many adjustable parameters: stationary phase chemistry, mobile phase composition, temperature, pH and of course by imposing a gradient mobile phase program [1][2][3][4][5][6][7][8][9][10]. Most gradient programs used in practice are simply linear.…”

On the inherent data fitting problems encountered in modeling retention behavior of analytes with dual retention mechanism

“…(1)-(4) with known coefficients as the input for k 1 and k 2 (the different considered cases and their parameter values are given in Table 1). In the case of gradient elution, retention times were predicted by numerically integrating the fundamental gradient equation [20], based on the trapezoid rule:…”

Prediction of the chromatographic retention of acid–base compounds in pH buffered methanol–water mobile phases in gradient mode by a simplified model

“…Some of them explain the behavior of neutral compounds in gradient mode and show several ways to predict their retention [1][2][3][4][5][6][7][8][9][10][11], others describe the isocratic retention of compounds with acid-base properties emphasizing the significance of the ionization degree of the analyte when predicting its retention [12][13][14][15], and there are some other studies that combine both the elution in gradient mode and the elution of ionizable compounds [16][17][18][19]. Combination of these two features is quite complex because the mobile phase * Corresponding author.…”