New approaches for the isolation of bifidobacterial strains, their molecular characterization, and assessment of their probiotic potential

Kharchenko, N. V.; Чердынцева, Т. А.; Netrusov, A. I.

doi:10.1134/s0026261715030108

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…The phosphoketolase activity encoded gene xfp has widely been using for identifying bifidobacteria as genus level (Yin et al, 2005). Several primers have been designed to amplify the gene of which xfp-F and xfp-R have recently been reported by Kharchenko et al (2015). The present results are in agreement with these studies.…”

Section: Genus Specific Identification Of Bifidobacteria Isolatessupporting

confidence: 92%

“…The genus specific identification of the purified isolates was performed according to the protocol described by Kharchenko et al (2015). The conventional PCR targeting the gene xfp was applied.…”

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

“…bacterial DNA Kit (Omega Bio-Tek, USA). The extracted DNA was then amplified by PCR (Eppendorf, Germany) using the primers xfp-F (5′-CGGCTGGCAGTCCAACAA-3′) and xfp-R (5′-GGTTGTTCTTGATGATGTCGG-3′) with an expected PCR product size of around 704 bp (Kharchenko et al, 2015). The PCR consisted of 35 cycles of 95°C for 30s, 57°C for 30s, and 72°C for 40s, with an initial denaturation (95°C for 5 min) and a final extension (72°C for 4 min).…”

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

“…The present results are in agreement with these studies. However, it is noteworthy that genus specific primer may not be reliable or sufficient to identify bifidobacteria (Kharchenko et al, 2015). Therefore, partial 16S rRNA gene sequencing is essential.…”

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

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Artisanal buffalo milk curd from Charfassion upazila of Bholadistrict in Bangladesh as a potent source of bifidobacteria

Habiba¹,

Jhinuk²,

Sumon³

et al. 2021

JAFE

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The present study explored the traditionally prepared fermented buffalo milk curd to isolate and characterize Bifidobacterium sp. towards developing future probiotics. Standard methods have been applied to identify Bifidobacterium sp. using bifidobacteria selective media, bifidobacteria specific mupirocin resistance test, phosphoketolase activity and commercial biochemical kit. The strains further confirmed by xfp gene PCR amplification. The growth dynamics of the identified isolates was also determined by monitoring the turbidity of the cultured media spectrophotometrically. A total of 20 isolates obtained from selective media were found gram positive, catalase negative, rod or v-shaped, non-motile and identified as presumptive bifidobacteria. All the isolates were resistant (zone diameter <10 mm) to the antibiotic mupirocin. However, the phosphoketolase activity of the isolates were varied significantly (p<0.05). A high, moderate and weak/no phosphoketolase activity was exhibited by 35%, 20% and 45% isolates, respectively. In the contrary, fourteen isolates (70%) amplified well with phosphoketolase activity encoded xfp gene and produced a PCR product of 704 bp. Combining the findings of these three tests, only six isolates (30%) displayed mupirocin resistance, high phosphoketolase activity and amplification with xfp gene. Of these six isolates biochemical test confirmed three isolates as Bifidobacterium mongoliense and two as Bifidobacterium pseudolongum subsp. globosum. The exponential phase growth rate of the six isolates also varied significantly (p<0.05) and ranged from 0.16±0.01/h to 0.34±0.02/h. Traditionally prepared buffalo milk curd from Charfassion upazila of Bhola district, Bangladesh could be a potent source of Bifidobacterium sp. However, species-specific identification and elucidation of probiotic potential are pivotal for future industrial use.

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Section: Genus Specific Identification Of Bifidobacteria Isolatessupporting

confidence: 92%

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

Section: Genus Specific Identification Of Bifidobacteria Isolatesmentioning

confidence: 99%

See 2 more Smart Citations

Artisanal buffalo milk curd from Charfassion upazila of Bholadistrict in Bangladesh as a potent source of bifidobacteria

Habiba¹,

Jhinuk²,

Sumon³

et al. 2021

JAFE

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“…No effect on mode of delivery was observed on the dominance of B. breve [ 29 ]. Kharchenko et al [ 32 ] isolated B. animalis subsp. lactis using culture dependent technique from faeces of new born infant.…”

Section: Discussionmentioning

confidence: 99%

Development and diversity of lactic acid producing bacteria and bifidobacteria in healthy full term Indian infants from Himachal Pradesh

2018

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Background/AimsThe initial microbial colonization is a crucial step for the healthy development of an infant. Previous studies from India reported the dominance of target microbial species among Indian infants without any analysis on the diversity of target groups. This is the first study from India with an objective to investigate the establishment and diversity of lactic acid producing bacteria (LAB) and bifidobacteria in vaginally delivered, full term, breastfed infants for the first 4 months after birth.MethodsPresent study used polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) based sequence analysis of LAB and bifidobacteria in healthy infants. The results were used to compare the development and early colonization by LAB and bifidobacteria using diversity indices during the initial months of development of gut microbiota in infants.ResultsDuring the first 4 months, the Shannon diversity index (H) of LAB increased from 1.16 to 1.318 and for bifidobacteria the H increased from 0.975 to 1.293 (P<0.05). Higher Sorenson’s pair wise similarity coefficient was observed for LAB and bifidobacteria during 2nd and the 3rd month. The species of the genera Enterococcus, Streptococcus, and Lactobacillus were dominant among the LAB group whereas Bifidobacterium breve was dominant species among Bifidobacterium group.ConclusionsOur results indicate that in breast fed infants, the microbial diversity of LAB and bifidobacteria increased during the period of study.

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Development of lyophilization procedure ensuring survival of bifidobacteria and preservation of their probiotic potential upon long-term storage

Kharchenko¹,

Cherdynceva

Нетрусов

2017

Microbiology

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New approaches for the isolation of bifidobacterial strains, their molecular characterization, and assessment of their probiotic potential

Cited by 3 publications

References 16 publications

Artisanal buffalo milk curd from Charfassion upazila of Bholadistrict in Bangladesh as a potent source of bifidobacteria

Artisanal buffalo milk curd from Charfassion upazila of Bholadistrict in Bangladesh as a potent source of bifidobacteria

Development and diversity of lactic acid producing bacteria and bifidobacteria in healthy full term Indian infants from Himachal Pradesh

Development of lyophilization procedure ensuring survival of bifidobacteria and preservation of their probiotic potential upon long-term storage

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