New approaches for long-term conservation of rooster spermatozoa

Janošíková, Martina; Petričáková, Kristýna; Ptáček, Martin; Savvulidi, Filipp; Rychtářová, Jana; Fulka, Josef

doi:10.1016/j.psj.2022.102386

Cited by 5 publications

(4 citation statements)

References 157 publications

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“…Poultry semen freezability is conditioned by many factors, including variations among breeds and lines [53][54][55][56]. Using the same freezing protocol implemented in the present study, 9% fertility associated with 41% viable embryos was reported in a commercial egglaying line [34].…”

Section: Discussionmentioning

confidence: 54%

Selection of Male Donors in Local Chicken Breeds to Implement the Italian Semen Cryobank: Variability in Semen Quality, Freezability and Fertility

Madeddu,

Zaniboni,

Marelli

et al. 2024

Veterinary Sciences

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Native breed conservation is an important component of poultry biodiversity. The aim of this work is to describe different steps that lead to donor selection for the implementation of the Italian Semen Cryobank of Autochthonous Chicken and Turkey Breeds. The variability within and between breeds was evaluated, and the stored semen reproductive capacity was in vivo tested using artificial insemination. Semen from Bionda Piemontese, Bianca di Saluzzo and Pepoi roosters was collected and processed. Concentration, volume, sperm membrane integrity, total motile sperm, progressive motile sperm and kinetic parameters were analyzed; sperm parameters accounting for bird variability were used to select male donors. Fresh semen quality parameters measured in donor ejaculates showed significant differences between breeds; no differences were found after cryopreservation. Variability in the fertilizing ability of cryopreserved semen was found within a breed (5–16%) and between birds within a breed (BP = 3–7%; BS = 7–31%; PP = 6–22%); only sperm quality parameters measured in fresh ejaculates, not frozen/thawed, may be associated with in vivo fertility results. In conclusion, sperm concentration and progressive motility were successfully used as selection parameters to identify chicken male donors with improved sperm quality for sperm cryobanking. However, new reliable sperm markers to predict cryopreserved semen’s fertilizing ability are required.

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Section: Discussionmentioning

confidence: 54%

Selection of Male Donors in Local Chicken Breeds to Implement the Italian Semen Cryobank: Variability in Semen Quality, Freezability and Fertility

Madeddu,

Zaniboni,

Marelli

et al. 2024

Veterinary Sciences

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“…Trehalose is a naturally occurring, non-toxic compound found in bacteria, fungi, plants, and invertebrates, which has been shown to provide cellular protection under a variety of stresses [26], including thermal, osmotic, and oxidative [13,[26][27][28][29]. Among the molecules that promote stability, trehalose provides the best membrane protection both in vivo and in vitro [5,27]. It is known that the response to stress caused by the freeze/thaw protocol is the rearrangement of membrane lipids in spermatozoa due to changes in the ratio of major lipids as well as changes in minor lipids.…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of sperm in poultry farming is still in the field of scientific research since the level of fertilizing ability of frozen-thawed sperm for use in industrial poultry farming has not been reached. Nevertheless, it should be noted that these technologies are already used in programs for the conservation of poultry genetic resources in many countries; however, the quality of reproductive material stored in cryobanks varies greatly and cannot always be successfully used for practical purposes [1][2][3][4][5]. The reproductive cells of male birds are very sensitive to various kinds of manipulations, especially at low temperatures.…”

Section: Introductionmentioning

confidence: 99%

Trehalose as a Stabilizer of the Lipid Composition of Membranes and the Composition of the Cytosol of Frozen/Thawed Rooster Spermatozoa

et al. 2023

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Low-temperature semen storage technologies are already being used in poultry conservation programs, but the quality of reproductive material stored in cryobanks varies greatly and cannot always be successfully used for practical purposes. Therefore, it is necessary to improve the compositions of cryoprotective media to improve their quality. This study aimed to investigate the composition of membrane lipids and carbohydrates in the cytosol of rooster spermatozoa, to explain the dose-dependent effect of a combination of trehalose and fructose in cryoprotective media on the preservation of their morphological and kinetic parameters during freezing/thawing, and to determine the most effective diluent composition. Ejaculates were collected from Rhode Island Red roosters (n = 10). The effectiveness of three diluents containing trehalose was evaluated: LCM-control (0 mM), Treh20 (9.5 mM), and Treh30 (13.4 mM). Chromatographic analysis of membrane lipids, carbohydrates, and polyols of the spermatozoa cytosol was performed. A decrease in the content of glycolipids in the plasma membranes of spermatozoa from 2.0% in native spermatozoa to 1.1–1.4% (frozen/thawed) and phospholipids from 71.2% (native) to 70.5% (frozen/thawed) reduced the progressive sperm motility from 65.7% in native spermatozoa to 12.6–27.6% (frozen/thawed). The same dynamics were observed for the viability parameter of 90.4% (native) and 27.0–41.2% (frozen/thawed). The Treh20 diluent, using a combination of fructose (36 mM) and trehalose (9.5 mM) saccharides, maximally preserved the lipid profile of plasma membranes and the composition of the cytosol of frozen/thawed rooster spermatozoa, which positively affected the indicators of general and progressive mobility and viability.

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“…This part of the experiment was run on species whose large sperm heads [40][41][42][43][44][45] allowed easy evaluation of the acrosome, i.e., the dog, bull, stallion and boar. Three different donors were used per species tested (three dogs, three bulls, three stallions and three boars) in order to run the analyses in triplicates.…”

Section: Cav Validation As a Marker Of Acrosomal Membrane Integrity (...mentioning

confidence: 99%

Validation of Calcein Violet as a New Marker of Semen Membrane Integrity in Domestic Animals

Egyptien

Dewals

Ectors

et al. 2023

Animals

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Many fluorochromes routinely used in semen quality analysis emit in the green and red channels, limiting their possible combination for multiple parameter analysis. The use of fluorophores emitting in different light channels broadens the possibilities of combination to expand the range of simultaneously evaluated criteria. This is of great interest in cases of small ejaculated volumes, such as those naturally occurring in roosters, small dog breeds and drones (Apis mellifera). The purpose of this experiment is to establish Calcein Violet (CaV), a blue fluorochrome, as a marker of viability and acrosomal integrity in domestic animals in order to free the red and green channels. SYBR®14/Propidium Iodide (PI) was used as reference dye, heat-treated samples as negative controls, serial staining combination for validation and epifluorescence microscopy for observation. Dead spermatozoa marked in red with PI showed no blue fluorescence either from the head or the tail. Live spermatozoa showed a decreasing blue emission from head to tail when single stained with CaV. Unreacted acrosomes showed intense blue fluorescence irrespective of plasma membrane integrity. This needs to be further confirmed for species with small and difficult to observe heads. Establishment of CaV as a marker of membrane integrity by fluorescence microscopy is a decisive first step towards further technical development and use with flow cytometry.

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New approaches for long-term conservation of rooster spermatozoa

Cited by 5 publications

References 157 publications

Selection of Male Donors in Local Chicken Breeds to Implement the Italian Semen Cryobank: Variability in Semen Quality, Freezability and Fertility

Selection of Male Donors in Local Chicken Breeds to Implement the Italian Semen Cryobank: Variability in Semen Quality, Freezability and Fertility

Trehalose as a Stabilizer of the Lipid Composition of Membranes and the Composition of the Cytosol of Frozen/Thawed Rooster Spermatozoa

Validation of Calcein Violet as a New Marker of Semen Membrane Integrity in Domestic Animals

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