New approach to modification of poly (l-lactic acid) with nano-hydroxyapatite improving functionality of human adipose-derived stromal cells (hASCs) through increased viability and enhanced mitochondrial activity

Śmieszek, Agnieszka; Marycz, Krzysztof; Szustakiewicz, Konrad; Kryszak, Bartłomiej; Targońska, Sara; Zawisza, Katarzyna; Watras, Adam; Wiglusz, Rafał J.

doi:10.1016/j.msec.2018.12.099

Cited by 31 publications

(28 citation statements)

References 65 publications

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“…The PLLA scaffold was a lack of cytocompatibility, thus the reason might be that HAP was a kind of bioactive ceramic rich in Ca 2+ and (PO 4 ) 3− to enhance the binding of cell and scaffold [50]. In our experiment, the adhesion, as well as proliferation of MG-63 cells, was fairly good by adding 20% C-HAP into the PLLA matrix, but in the study of Smieszek et al [51], porous PLLA scaffolds with 10 wt % HAP could significantly improve the adhesion and proliferation of adipose-derived stromal cells. Our results differ, not only because of the methods used to prepare bone scaffold, but also because MG-63 cells have special cellular physiological characteristics.…”

Section: Bioactivity and Cytocompatibilitycontrasting

confidence: 51%

“…Some reports showed that interconnected and porous structures of the scaffold had the potential to support cell proliferation and growth [48,49]. HAP into the PLLA matrix, but in the study of Smieszek et al [51], porous PLLA scaffolds with 10 wt % HAP could significantly improve the adhesion and proliferation of adipose-derived stromal cells. Our results differ, not only because of the methods used to prepare bone scaffold, but also because MG-63 cells have special cellular physiological characteristics.…”

Section: Bioactivity and Cytocompatibilitymentioning

confidence: 99%

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Phosphonic Acid Coupling Agent Modification of HAP Nanoparticles: Interfacial Effects in PLLA/HAP Bone Scaffold

Shuai

Yang

et al. 2020

Polymers

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In order to improve the interfacial bonding between hydroxyapatite (HAP) and poly-l-lactic acid (PLLA), 2-Carboxyethylphosphonic acid (CEPA), a phosphonic acid coupling agent, was introduced to modify HAP nanoparticles. After this. the PLLA scaffold containing CEPA-modified HAP (C-HAP) was fabricated by selective laser sintering (frittage). The specific mechanism of interfacial bonding was that the PO32− of CEPA formed an electrovalent bond with the Ca2+ of HAP on one hand, and on the other hand, the –COOH of CEPA formed an ester bond with the –OH of PLLA via an esterification reaction. The results showed that C-HAP was homogeneously dispersed in the PLLA matrix and that it exhibited interconnected morphology pulled out from the PLLA matrix due to the enhanced interfacial bonding. As a result, the tensile strength and modulus of the scaffold with 20% C-HAP increased by 1.40 and 2.79 times compared to that of the scaffold with HAP, respectively. In addition, the scaffold could attract Ca2+ in simulated body fluid (SBF) solution by the phosphonic acid group to induce apatite layer formation and also release Ca2+ and PO43− by degradation to facilitate cell attachment, growth and proliferation.

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Section: Bioactivity and Cytocompatibilitycontrasting

confidence: 51%

Section: Bioactivity and Cytocompatibilitymentioning

confidence: 99%

Phosphonic Acid Coupling Agent Modification of HAP Nanoparticles: Interfacial Effects in PLLA/HAP Bone Scaffold

Shuai

Yang

et al. 2020

Polymers

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“…The protocol was approved by the Local Bioethics Committee of Wroclaw Medical School (registry number KB-177/2014, Wroclaw, Poland). Primary cultures of hASCs were propagated following our previous well-established protocol [33,38]. Immunophenotyping of hASCs was performed after third passage (p3).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the nHAp can be modified with an active antibacterial agent (e.g., tetracycline) promoting simultaneously viability and proliferative activity of MSCs [31]. Furthermore, the nHAp effectively promotes the osteogenic differentiation potential of the MSCs, and could be used as an efficient tool for drug and/or a bioactive agent delivery system [32,33]. It is worth noting that nHAp doped with lanthanide ions also exhibits luminescent ability, which makes them an excellent candidate for bioimaging [34].…”

Section: Introductionmentioning

confidence: 99%

Nanocrystalline Hydroxyapatite Loaded with Resveratrol in Colloidal Suspension Improves Viability, Metabolic Activity and Mitochondrial Potential in Human Adipose-Derived Mesenchymal Stromal Stem Cells (hASCs)

et al. 2019

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In response to the demand for new multifunctional materials characterized by high biocompatibility, hydrogel (HG) nanocomposites as a platform for bioactive compound delivery have been developed and fabricated. A specific crosslinking/copolymerization chemistry was used to construct hydrogels with a controlled network organization. The hydrogels were prepared using 3,6-anhydro-α-l-galacto-β-d-galactan (galactose hydrogel) together with resveratrol (trans-3,5,4′-trihydroxystilbene) and calcium hydroxyapatite nanoparticles. The resveratrol was introduced in three different concentrations of 0.1, 0.5, and 1 mM. Nanosized calcium hydroxyapatite was synthesized by a microwave-assisted hydrothermal technique, annealed at 500 °C for 3 h, and introduced at a concentration 10% (m/v). The morphology and structural properties of Ca10(PO4)6(OH)2 and its composite were determined by using XRPD (X-ray powder diffraction) techniques, as well as the absorption and IR (infrared) spectroscopy. The average nanoparticle size was 35 nm. The water affinity, morphology, organic compound release profile, and cytocompatibility of the obtained materials were studied in detail. The designed hydrogels were shown to be materials of biological relevance and of great pharmacological potential as carriers for bioactive compound delivery. Their cytocompatibility was tested using a model of human multipotent stromal cells isolated from adipose tissue (hASCs). The biomaterials increased the proliferative activity and viability of hASCs, as well as reduced markers of oxidative stress. In light of the obtained results, it has been thought that the designed materials meet the requirements of the tissue engineering triad, and may find application in regenerative medicine, especially for personalized therapies.

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“…The concentration of protein was evaluated using the Bicinchoninic Acid Assay Kit (Sigma Aldrich, Munich, Germany). The procedure of the reaction was established previously [22,30]. The concentration of proteins loaded per well was equal to 20 µg.…”

Section: Western Blotting Detection Of Osteopontin and Runx-2mentioning

confidence: 99%

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Śmieszek

Marcinkowska

Pielok

et al. 2020

Cells

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MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts’ differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts.

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New approach to modification of poly (l-lactic acid) with nano-hydroxyapatite improving functionality of human adipose-derived stromal cells (hASCs) through increased viability and enhanced mitochondrial activity

Cited by 31 publications

References 65 publications

Phosphonic Acid Coupling Agent Modification of HAP Nanoparticles: Interfacial Effects in PLLA/HAP Bone Scaffold

Phosphonic Acid Coupling Agent Modification of HAP Nanoparticles: Interfacial Effects in PLLA/HAP Bone Scaffold

Nanocrystalline Hydroxyapatite Loaded with Resveratrol in Colloidal Suspension Improves Viability, Metabolic Activity and Mitochondrial Potential in Human Adipose-Derived Mesenchymal Stromal Stem Cells (hASCs)

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

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