Abstract:The aim of this study is developing and validation of UV-spectrophotometric method for determination of taurine in energy drink mixtures. The investigation includes validation of procedures for performance of the tests for identification, purity and assay. Analytical parameters precision, accuracy, selectivity, linearity, limit of detection and limit of quantitation were studied and compared. Developed method allows selectively determination of taurine in the present of caffeine at different conditions (algorithm, wavelength zones). The preferences are estimation the analytical parameters from the validation procedures. They let to compose the criteria in accordance with European Pharmacopoeia and EU regulates about the use of official analytical programs for quality control of supplements containing taurine. The method has been applied successfully in analysis of energy food drinks preparation in the appointed for each of their aspect of applications.