New Approach for the Construction and Calibration of Gas-Tight Setups for Biohydrogen Production at the Small Laboratory Scale

Autenrieth, Caroline; Shaw, Shreya; Ghosh, Robin

doi:10.3390/metabo11100667

Cited by 3 publications

(1 citation statement)

References 33 publications

(53 reference statements)

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“…Moreover, it has been crucial in the elucidation of the basic fundaments of polyhydroxyalkanoates (PHAs) metabolism ( 25 – 27 ) showing recently a great potential for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from fructose in anaerobic conditions ( 28 ). It has also been proposed as a biocatalyst for the production of hydrogen ( 29 – 32 ), pigments, and vitamins ( 33 ). In this microorganism, PS regulation shows particular features compared to other PNSBs.…”

Section: Introductionmentioning

confidence: 99%

A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state

Godoy,

de Miguel,

Prieto

2023

mSystems

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Photosynthesis (PS) in purple non-Sulfur bacteria (PNSB) is strictly controlled to avoid energy consumption and to prevent oxidative stress when oxygen is present. PpsR, a transcriptional repressor encoded in the photosynthesis gene cluster (PGC), is one of the main players in the regulation of PS-related genes. In some cases, PpsR does not sense the environmental cue by itself but delegates this task to other proteins like PpaA/AerR anti-repressors, which can ultimately affect PpsR affinity to some promoter regions. In this work, the effects of locus Rru_A0625 product (HP1) on PS regulation of Rhodospirillum rubrum were studied by mutation/complementation and transcriptomic strategies. Rru_A0625 is located next to ppsR gene, just like other PpaA/AerR members, and its deletion annuls pigment synthesis in dark micro/anaerobic growth conditions. HP1 shows similarity to PpaA/AerR anti-repressors family, although it does not possess their typical cobalamin binding domain. A transcriptomic analysis of Rru_A0625 deletion mutant showed that HP1 not only has effects on bacteriochlorophyll and carotenoid biosynthesis, but also many other biological processes in the cell. The most notorious is the impact on the transcription of the nitrogenase complex components and accessory proteins. Our results suggest that this new member of the PpaA/AerR family has evolved losing the canonical cobalamin binding domain, but not the redox sensing capability, conserving a not fully understood mechanism of PS regulation. IMPORTANCE Rhodospirillum rubrum vast metabolic versatility places it as a remarkable model bacterium and an excellent biotechnological chassis. The key component of photosynthesis (PS) studied in this work (HP1) stands out among the other members of PpaA/AerR anti-repressor family since it lacks the motif they all share: the cobalamin B-12 binding motif. Despite being reduced and poorly conserved, HP1 stills controls PS as the other members of the family, allowing a fast response to changes in the redox state of the cell. This work also shows that HP1 absence affects genes from relevant biological processes other than PS, including nitrogen fixation and stress response. From a biotechnological perspective, HP1 could be manipulated in approaches where PS is not necessary, such as hydrogen or polyhydroxyalkanoates production, to save energy.

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Section: Introductionmentioning

confidence: 99%

A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state

Godoy,

de Miguel,

Prieto

2023

mSystems

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Recent advances in process improvement of dark fermentative hydrogen production through metabolic engineering strategies

Krishnan

Kamyab

Nasrullah

et al. 2023

Fuel

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Process Model and Life Cycle Assessment of Biorefinery Concept Using Agricultural and Industrial Residues for Biohydrogen Production

Gamero,

Ruppert,

Miehe

et al. 2024

Energies

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Sustainable waste management strategies are urgently needed due to an increasing global population and increased waste production. In this context, biorefineries have recently emerged as a promising approach to valorize waste streams and supply a broad range of products. This study presents the process model and life cycle assessment (LCA) of a biorefinery concept using a novel biochemical method, a so-called “dark photosynthesis” conversion. This process is coupled to a photo-fermentation using microalgae. Overall, the biorefinery concept can produce hydrogen, lutein, β-carotene, and proteins for animal feed. Apple pomace from apple juice production is used as feedstock for the primary conversion step. A process model was created with the process simulation software Aspen Plus® using experimental and literature data. Results from this model were then used in an LCA. The environmental impacts of the proposed biorefinery concept are relatively high, showing the need for process optimization in several areas. Energy system integration, stream recycling, and higher hydrogen yields are recognized as especially important for improving the environmental performance of this concept. Despite these findings, the model shows the feasibility of implementing the biochemical conversion technologies in a biorefinery concept for effectively utilizing residue streams.

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New Approach for the Construction and Calibration of Gas-Tight Setups for Biohydrogen Production at the Small Laboratory Scale

Cited by 3 publications

References 33 publications

A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state

A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state

Recent advances in process improvement of dark fermentative hydrogen production through metabolic engineering strategies

Process Model and Life Cycle Assessment of Biorefinery Concept Using Agricultural and Industrial Residues for Biohydrogen Production

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