New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence

Palmieri, Gianna; Balestrieri, Marco; Proroga, Yolande T.R.; Falcigno, Lucia; Facchiano, Angelo; Riccio, Alessia; Capuano, Federico; Marrone, Raffaele; Neglia, Gianluca; Anastasio, Aniello

doi:10.1016/j.foodchem.2016.05.100

Cited by 34 publications

(24 citation statements)

References 38 publications

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“…Structure calculations for MTP1 and MTP2 performed by the standard CYANA simulated annealing schedule [9] were carried out by using NMR data evaluated in H 2 O/TFE-d 3 1:1, as reported in [1] . Statistical data of calculations are reported in Table 5 , Table 6 .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“… Here we report details on the design and conformational analysis of two novel peptides showing antimicrobial properties, as reported in the research article, “New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence” G. Palmieri, M. Balestrieri, Y.T.R. Proroga, L. Falcigno, A. Facchiano, A. Riccio, F. Capuano, R. Marrone, G. Campanile, A. Anastasio (2016) [1] . NMR data, such as chemical shifts in two different solvents as well as aCH protons deviations from random coil values and NOE patterns, are shown together with the statistics of structural calculations.…”

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confidence: 99%

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NMR and computational data of two novel antimicrobial peptides

et al. 2016

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Here we report details on the design and conformational analysis of two novel peptides showing antimicrobial properties, as reported in the research article, “New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence” G. Palmieri, M. Balestrieri, Y.T.R. Proroga, L. Falcigno, A. Facchiano, A. Riccio, F. Capuano, R. Marrone, G. Campanile, A. Anastasio (2016) [1]. NMR data, such as chemical shifts in two different solvents as well as aCH protons deviations from random coil values and NOE patterns, are shown together with the statistics of structural calculations. Strategy and particulars of molecular design are presented.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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confidence: 99%

NMR and computational data of two novel antimicrobial peptides

et al. 2016

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“…In this study, an immobilization platform on PET surfaces by using the previously characterized AMP MTP1 (Palmieri et al, 2016) was developed, exposing PET disks to a radiofrequency cold plasma by changing the main parameters of the process including the O 2 exposure time (T), RF power, and the concentration/the partial pressure (P) of molecular oxygen O 2 . To assess the surface hydrophilicity of the PET slides after treatments, water contact angle (WCA) measurements were performed (Figure 1).…”

Section: Activation and Functionalization Of Pet Polymer By Cold Plasmentioning

confidence: 99%

“…As many naturally occurring peptides lack the ability to retain these properties, there is a need to develop new and more effective AMPs, with the aim to increase safety and shelf-life of food products. Recently, starting from the human source sequence of CPT-1a (McGarry and Brown, 1997), a new AMP, named mitochondrial-targeted peptide 1 (MTP1), was designed as already stated in Palmieri et al (2016) and characterized. Specifically, the 15-mer peptide was revealed to be highly stable in a broad range of pH (2-10) and temperature (15-90 • C) for prolonged incubation times.…”

Section: Introductionmentioning

confidence: 99%

Extending the Shelf-Life of Meat and Dairy Products via PET-Modified Packaging Activated With the Antimicrobial Peptide MTP1

et al. 2020

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Fresh products are characterized by reduced shelf-life because they are an excellent growth medium for a lot of microorganisms. Therefore, the microbial spoilage causing significant food supply losses has become an enormous economic and ethical problem worldwide. The antimicrobial packaging is offering a viable solution to tackle this economic and safety issue by extending the shelf-life and improving the quality and safety of fresh products. The goal of this study was to investigate the effects of a food contact surface of polyethylene terephthalate (PET) functionalized with the previously characterized antimicrobial peptide mitochondrial-targeted peptide 1 (MTP1), in reducing the microbial population related to spoilage and in providing the shelflife stability of different types of fresh foods such as ricotta cheese and buffalo meat. Modified polymers were characterized concerning the procedure of plasma-activation by water contact angle measurements and Fourier transform infrared spectroscopy measurements in attenuated total reflection mode (ATR-FTIR). Results showed that the MTP1-PETs provided a strong antimicrobial effect for spoilage microorganisms with no cytotoxicity on a human colon cancer cell line. Finally, the activated polymers revealed high storage stability and good reusability. This study provided valuable information to develop alternative antimicrobial packaging for enhancing and extending the microbial quality and safety of perishable foods during storage.

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“…The structure-activity relationship (SAR) of AMPs is a key determinant because it provides information about the molecular mechanisms that regulate the AMPs' biological activity over pathogens [19]. Up until now, it has been understood that the antimicrobial capacity of many peptides is dependent on different biophysical properties that allow the adsorption, permeation, and rupture of microorganisms' cell walls [20 ]. The main differences among AMPs are related to their low homology in amino acid sequences and their wide variety of secondary structures [18 ].…”

Section: Structural Diversity Of Ampsmentioning

confidence: 99%

Gut microbiota and antimicrobial peptides

Sivieri¹,

Bassan²,

Peixoto³

2017

Current Opinion in Food Science

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This mini-review covers the potential interactions between gut microbiota and antimicrobial peptides, as well as the main mechanisms of action of antimicrobial peptides. One of the most efficient molecules produced by resident gut microbiota are peptides, which have antimicrobial functions and an innate immune response against infectious agents. Currently, more than 2300 AMPs have been isolated. The human gut microbiota regulates the production of defensins, cathelicidins, C-type lectins, ribonucleases, and S100 proteins in intestinal epithelial cells and Paneth cells, which rapidly kill or inactivate invading microorganisms. The study of gut microbiota and new peptides provides perspective for the synthesis of analogous molecules as an alternative in solving the problem of multidrug resistance reported with conventional antibiotics.

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New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence

Cited by 34 publications

References 38 publications

NMR and computational data of two novel antimicrobial peptides

NMR and computational data of two novel antimicrobial peptides

Extending the Shelf-Life of Meat and Dairy Products via PET-Modified Packaging Activated With the Antimicrobial Peptide MTP1

Gut microbiota and antimicrobial peptides

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