New antibiotic-producing Streptomyces TT-strain, generated by electrical fusion of protoplasts

Okamura, Tokumitsu; Nagata, Shinji; Misono, Haruo; Nagasaki, Susumu

doi:10.1016/0922-338x(89)90222-5

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…Given that only a small fraction of the estimated 1.5 million species of fungi have been described [ 110 ] and that only a handful of these have been subjected to protoplast fusion experiments, it seems probable that by combining genomes of these fungi, new biosynthetic pathways, and therefore, new antibiotics, could be synthesized. Furthermore, novel antibiotics have been produced by protoplast fusion of bacteria [ 111 , 112 ], suggesting this approach may eventually prove fruitful in fungi.…”

Section: Pharmaceutical Productionmentioning

confidence: 99%

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Strom

Bushley

2016

Fungal Biol Biotechnol

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Heterokaryosis is an integral part of the parasexual cycle used by predominantly asexual fungi to introduce and maintain genetic variation in populations. Research into fungal heterokaryons began in 1912 and continues to the present day. Heterokaryosis may play a role in the ability of fungi to respond to their environment, including the adaptation of arbuscular mycorrhizal fungi to different plant hosts. The parasexual cycle has enabled advances in fungal genetics, including gene mapping and tests of complementation, dominance, and vegetative compatibility in predominantly asexual fungi. Knowledge of vegetative compatibility groups has facilitated population genetic studies and enabled the design of innovative methods of biocontrol. The vegetative incompatibility response has the potential to be used as a model system to study biological aspects of some human diseases, including neurodegenerative diseases and cancer. By combining distinct traits through the formation of artificial heterokaryons, fungal strains with superior properties for antibiotic and enzyme production, fermentation, biocontrol, and bioremediation have been produced. Future biotechnological applications may include site-specific biocontrol or bioremediation and the production of novel pharmaceuticals.

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Section: Pharmaceutical Productionmentioning

confidence: 99%

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Strom

Bushley

2016

Fungal Biol Biotechnol

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“…and Bacillus subtilis was first reported by Gokhale et al (1984) and this fusion technique has been used for enhancement of lignin degradation (Pettey and Crawford, 1984), amino acid production (Karasawa et al 1986), production of new antibiotics (Okamura et al 1989) and thermostable amylase (Gokhale and Deobagkar, 1994). and Bacillus subtilis was first reported by Gokhale et al (1984) and this fusion technique has been used for enhancement of lignin degradation (Pettey and Crawford, 1984), amino acid production (Karasawa et al 1986), production of new antibiotics (Okamura et al 1989) and thermostable amylase (Gokhale and Deobagkar, 1994).…”

Section: Introductionmentioning

confidence: 99%

Intergeneric protoplast fusion between Acinetobacter sp. A3 and Pseudomonas putida DP99 for enchanced hydrocarbon degradation

Hanson

Desai

1996

Biotechnol Lett

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Polyethyleneglycol 6000 mediated protoplast fusion between an alkane degrader Acinetobacter sp. A3, and a naphthalene degrader, Pseudomonas putida DP99 1 resulted in fusants capable of degrading both hydrocarbons and were morphologically similar to Acinetobacter sp. A3. While fusant F4/13 and Pseudomonas putida DP99 degraded over 98% of naphthalene provided by the end of five days, tetradecane degradation by fusant F4113 was 82% compared to 77% by Acinefobacter sp. A3 in the same time period. Also, while from naphthalene +tetradecane mixture, fusant F4/13 could degrade 99% and 53% of naphthalene and tetradecane respectively, both the parent strains together could degrade over 99% naphthalene but only about 16% tetradecane.

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Cell Fusion

Anné¹

1992

Biotechnology

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New antibiotic-producing Streptomyces TT-strain, generated by electrical fusion of protoplasts

Cited by 6 publications

References 16 publications

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Intergeneric protoplast fusion between Acinetobacter sp. A3 and Pseudomonas putida DP99 for enchanced hydrocarbon degradation

Cell Fusion

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