New Antibacterial Steroidal Alkaloids fromSarcococca Brevifolia

New Cholinesterase Inhibiting Steroidal Alkaloids from the Leaves of Sarcococca coriacea of Nepalese Origin.

“…The spectral data of compound 3 has been presented for the first time in this paper. Compound 4 (C 22 H 37 NO, HR-EI-MS m/z 331.2806) 16) was also isolated for the first time from this genus as a yellowish crystalline solid. This compound was earlier isolated from the leaves of Holarrhena febrifuga.…”

“…This compound was earlier isolated from the leaves of Holarrhena febrifuga. 16) The stereochemical assignments of stereogenic centers in the compounds 1-4 were made on the basis of nuclear Overhauser effect spectroscopy (NOESY) interactions, chemical shifts comparisions with reported compounds [9][10][11][12][13][14][15][16][17][18] and biogenetic considerations keeping in view of the fact that all pregnane-type steroidal alkaloids are biosynthesized from cholesterol via pregnenolone. 19) The b-orientation of the C-20 methine proton in compounds 1-3, and equatorial position of the C-3 tigloyl group in compounds 1-2 were confirmed by comparing the spectral data with reported compounds.…”

“…19) The b-orientation of the C-20 methine proton in compounds 1-3, and equatorial position of the C-3 tigloyl group in compounds 1-2 were confirmed by comparing the spectral data with reported compounds. [9][10][11][12][13][14][15][16][17][18]25) In compounds 1 and 2, the cis configuration of tigloyl methyl groups at C-2Ј and C-3Ј were inferred from NOESY interactions between the C-3Ј olefinic proton with C-4Ј methyl protons.…”

“…6) We have previously reported a number of steroidal alkaloids from various species of Sarcococca. 8,14,[16][17][18] In our continuing studies on steroidal alkamines from this genus, we recently initiated work on S. coriacea (HOOK. f.) SWEET which is an evergreen shrub widely distributed in central Nepal.…”

A review of studies on bioactive compounds isolated from Sri Lankan flora

“…Antibacterial activity of epipachysamine-E-5-ene-4-on has been observed against Bacillus cereus (MIC = 0.0625 mg/mL), Klebsiella pneumoniae (MIC = 0.25 mg/mL) and Staphylococcus aureus (MIC = 0.03125 mg/mL). Similarly, iso-N-formylchonemorphin has shown antibacterial strong activity against Bacillus cereus (MIC = 0.1250 mg/mL), Pseudomonas aeruginosa (MIC = 0.1250 mg/mL) and Staphylococcus aureus (MIC = 0.0312 mg/mL) (Jayasinghe et al, 1998).…”