New animal model to evaluate testicular blood flow during testicular torsion

Lievano, Gonzalo; Nguyen, Ling; Radhakrishnan, Jayant; Fornell, Linda; John, Eunice

doi:10.1016/s0022-3468(99)90778-9

Cited by 39 publications

(31 citation statements)

References 4 publications

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“…In general, at least 4 h of 720°u nilateral testicular torsion causes enough testicular tissue injury in both ipsilateral and contralateral testes in experimental models [20,21]. Even though many experimental studies have clearly revealed that the contralateral nontorted testis is damaged after unilateral testicular torsion [22][23][24][25], some studies have shown that this phenomenon is not always observed [26,27].…”

Section: Discussionmentioning

confidence: 99%

The protective effect of selenium on ipsilateral and contralateral testes in testicular reperfusion injury

et al. 2005

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This study was designed to investigate the effect of selenium on ipsilateral and contralateral testicular damage after unilateral testicular torsion/detorsion (T/D). Thirty-two male rats were divided into four groups, each containing eight rats. Torsion was created by rotating the right testis 720 degrees in a clockwise direction. Group 1 underwent sham operation to determine basal values for biochemical and histopathological evaluation. Sham operation was performed in group 2, and sodium selenate (0.2 mg/kg) was given intraperitoneally. Group 3 served as a T/D group, receiving 4-h torsion and 4-h detorsion. Similarly, in group 4 sodium selenate (0.2 mg/kg) was injected intraperitoneally 20 min before detorsion. Bilateral orchiectomies were performed for measurement of tissue malondialdehyde (MDA) levels and superoxide dismutase (SOD) activities and histopathologic examination. The results were compared statistically. The highest MDA and the lowest SOD values were determined in both testes in group 3. There were statistically significant differences in MDA levels and SOD activities in group 3 compared with group 4. Specimens from group 3 had a significantly greater histologic injury than other groups. These results suggest that ischemia-reperfusion injury occurred in both testes after unilateral testicular T/D and that selenium administration before detorsion prevents reperfusion injury in testicular torsion.

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Section: Discussionmentioning

confidence: 99%

The protective effect of selenium on ipsilateral and contralateral testes in testicular reperfusion injury

et al. 2005

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“…However, even with successful surgical repair, testicular atrophy and infertility are a common clinical outcome [1]. The cause of this I-R injury may possibly be attributed to oxygen free radicals [2].…”

Section: Introductionmentioning

confidence: 99%

PAF antagonist BN-52021 reduces intercellular adhesion molecule-1 expression and oxidative stress in rats with reperfusion damage due to unilateral testicular torsion

Öztürk

Yağmur

2005

Ped Surgery Int

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The aim of this study was to determine the effects of specific platelet-activating factor (PAF) antagonist BN-52021 on intercellular adhesion molecule-1 (ICAM) expression and oxidative stress in rats with reperfusion damage due to unilateral testicular torsion. Thirty male Sprague-Dawley rats were separated into three groups, each containing ten rats. A sham operation was performed in group 1 (control). In group 2 [ischemia-reperfusion (I-R)/untreated], 1-h detorsion of the testis was performed after 6 h of unilateral testicular torsion. In group 3 (I-R/BN-52021), after performing the same surgical procedures as in groups II, BN-52021 was given intravenously at the starting time of reperfusion. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and measuring the tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). MDA values and the testicular injury score decreased and SOD, CAT and GSH-Px values increased in the I-R/BN-52021 treated group compared to in the I-R/untreated group. Most of the specimens in the I-R/BN-52021 treated group showed grade-I testicular injury. However, the injuries in the I-R/untreated rats varied between grades III and IV. An ICAM-1 expression was intensive in the interstitial spaces and basement membrane of the tubuli seminiferi, of testicular tissue in the I-R/untreated group. However, an ICAM-1 expression was mild in the I-R/BN-52021 group. BN-52021 may play an important role in the immunohistochemical expression of adhesion molecule ICAM-1 and may reduce oxidative stress in rats with reperfusion damage due to unilateral testicular torsion.

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“…Therefore, [16,17] one may suggest that as a testis and as epididymis undergo torsion and become ischemic; the cellular architecture of the testis is rapidly damaged. Several studies showed that I-R injury is caused by oxygen free radicals [4,18,19]. These free radicals promote cellular damage through the peroxidation of lipids in mitochondrial and cell membranes [18].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies showed that I-R injury is caused by oxygen free radicals [4,18,19]. These free radicals promote cellular damage through the peroxidation of lipids in mitochondrial and cell membranes [18]. It is known that malondialdehyde levels are always elevated during any free-radical mediated tissue damage and serves as a good marker of lipid peroxidation [19,20].…”

Section: Discussionmentioning

confidence: 99%