New animal model of extrinsic dental erosion-Erosive effect on the mouse molar teeth

Tulek, Amela; Saeed, Muhammad; Mulic, Aida; Stenhagen, Kjersti Refsholt; Utheim, Tor Paaske; Galtung, Hilde Kanli; Nirvani, Minou; Kristiansen, Marthe Smedmoen

doi:10.1016/j.archoralbio.2018.08.013

Cited by 7 publications

(20 citation statements)

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“…In addition to wear studies, frictional coefficients during flossing of teeth were measured [425]. Animal models [426] and in vitro and in vivo clinical models were developed [415,427]. The importance of saliva in oral tribology was also increasingly recognized, including changes in saliva due to age [428], dry mouth, and in vitro simulation system [429], effect of palm oil [430], and bio-lubricants [167].…”

Section: Oral Tribologymentioning

confidence: 99%

A review of recent advances in tribology

et al. 2020

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The reach of tribology has expanded in diverse fields and tribology related research activities have seen immense growth during the last decade. This review takes stock of the recent advances in research pertaining to different aspects of tribology within the last 2 to 3 years. Different aspects of tribology that have been reviewed including lubrication, wear and surface engineering, biotribology, high temperature tribology, and computational tribology. This review attempts to highlight recent research and also presents future outlook pertaining to these aspects. It may however be noted that there are limitations of this review. One of the most important of these is that tribology being a highly multidisciplinary field, the research results are widely spread across various disciplines and there can be omissions because of this. Secondly, the topics dealt with in the field of tribology include only some of the salient topics (such as lubrication, wear, surface engineering, biotribology, high temperature tribology, and computational tribology) but there are many more aspects of tribology that have not been covered in this review. Despite these limitations it is hoped that such a review will bring the most recent salient research in focus and will be beneficial for the growing community of tribology researchers.

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Section: Oral Tribologymentioning

confidence: 99%

A review of recent advances in tribology

et al. 2020

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“…SEM images of the transversely ground and etched plane T1 were used for measuring enamel thickness and tooth height in wild-type control, NOD control and experimental mandibular first molars, corresponding to our previous study [13]. Mean values and standard deviations were calculated using Microsoft Excel Worksheet.…”

Section: Discussionmentioning

confidence: 99%

“…Fifty pieces of Teklad Global 18% Protein Rodent Diet (Envigo Teklad, Madison, WI, USA) were soaked with 165 mL cold tap water, sealed in a plastic bag, and left to soften overnight. Furthermore, wire cages with solid bottom and bedding were prepared to reduce wear of the dentition by attrition, as described previously [13]. The cages were carefully inspected before the mice were transferred into them.…”

Section: Animal Modelmentioning

confidence: 99%

“…However, only a few studies have focussed on the risk indicators and preventive treatment related to dental erosion in animal models [7][8][9][10][11], and the criteria for the registration of dental erosive lesions were mainly semi-quantitative, and did not allow precise recording of small erosive lesions and their depth [9,12]. Recently, an animal model in which experimental dental erosion was induced in mouse with normal salivary flow was developed, and the erosive effect of products containing both citric and phosphoric acid was studied in detail [13]. The results showed that cola drink (phosphoric acid) exhibited higher erosive effects on mouse mandibular molars compared to sports drink (citric acid), and the presented method, with transversely ground molars observed under a scanning electron microscope (SEM), enabled comprehensive registration of erosive lesions and their depths in small teeth such as mouse molars [13].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, an animal model in which experimental dental erosion was induced in mouse with normal salivary flow was developed, and the erosive effect of products containing both citric and phosphoric acid was studied in detail [13]. The results showed that cola drink (phosphoric acid) exhibited higher erosive effects on mouse mandibular molars compared to sports drink (citric acid), and the presented method, with transversely ground molars observed under a scanning electron microscope (SEM), enabled comprehensive registration of erosive lesions and their depths in small teeth such as mouse molars [13].…”

Section: Introductionmentioning

confidence: 99%

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Dental erosion in mice with impaired salivary gland function

Tulek

Mulic

Stenhagen

et al. 2020

Acta Odontologica Scandinavica

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Objective: Salivary flow rate exerts an essential impact on the development and progression of dental erosion. In this work, the experimental dental erosion in non-obese diabetic (NOD) mice with reduced salivary flow rate was induced, and the erosive effect of acidic drinks on their dentition was studied. Material and methods: Three acidic drinks (sports drink, cola light drink and sugar containing cola drink) were given to adult NOD mice (groups: N ¼ 11) as the only drink for 6 weeks. Two control groups were included; wild type and NOD control (groups: N ¼ 9). Experimental and control (water) teeth were dissected out and observed by scanning electron microscopy (SEM). Mandibular first molars were subsequently embedded in Epon, ground transversely, observed again by SEM, and the enamel thickness and tooth height were measured. Results: Mandibular molars were considerably more eroded than maxillary molars. The erosive process started at the top of the cusps and subsequently extended in the cervical, mesio-distal, and pulpal direction. Erosive lesions were evident in increased succession from sports drink, cola light to cola drink exposed mandibular molars, with the lingual tooth height being approximately 23%, 26%, and 37% lower, respectively, compared to the control. The lingual enamel was approximately 48% thinner in sports drink molars and 62% thinner in cola light molars. In cola drink molars, the lingual enamel was totally eroded, and significant erosion of dentine was evident. Conclusion: Reduced salivary flow, together with a high consumption of acidic drinks, results in severe erosion of NOD mice molars.

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Dentoalveolar Alterations in an Adenine‐Induced Chronic Kidney Disease Mouse Model

Mohamed,

Amadeu de Oliveira,

Kinoshita

et al. 2023

J of Bone & Mineral Res

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Chronic kidney disease (CKD) is characterized by kidney damage and loss of renal function. CKD mineral and bone disorder (CKD–MBD) describes the dysregulation of mineral homeostasis, including hyperphosphatemia and elevated parathyroid hormone (PTH) secretion, skeletal abnormalities, and vascular calcification. CKD–MBD impacts the oral cavity, with effects including salivary gland dysfunction, enamel hypoplasia and damage, increased dentin formation, decreased pulp volume, pulp calcifications, and altered jaw bones, contributing to clinical manifestations of periodontal disease and tooth loss. Underlying mechanisms are not fully understood, and CKD mouse models commonly require invasive procedures with high rates of infection and mortality. We aimed to characterize the dentoalveolar effects of an adenine diet (AD)‐induced CKD (AD–CKD) mouse model. Eight‐week‐old C57BL/6J mice were provided either a normal phosphorus diet control (CTR) or adenine and high‐phosphorus diet CKD to induce kidney failure. Mice were euthanized at 15 weeks old, and mandibles were collected for micro–computed tomography and histology. CKD mice exhibited kidney failure, hyperphosphatemia, and hyperparathyroidism in association with porous cortical bone in femurs. CKD mice showed a 30% decrease in molar enamel volume compared to CTR mice. Enamel wear was associated with reduced ductal components, ectopic calcifications, and altered osteopontin (OPN) deposition in submandibular salivary glands of CKD mice. Molar cusps in CKD mice were flattened, exposing dentin. Molar dentin/cementum volume increased 7% in CKD mice and pulp volume decreased. Histology revealed excessive reactionary dentin and altered pulp‐dentin extracellular matrix proteins, including increased OPN. Mandibular bone volume fraction decreased 12% and bone mineral density decreased 9% in CKD versus CTR mice. Alveolar bone in CKD mice exhibited increased tissue‐nonspecific alkaline phosphatase localization, OPN deposition, and greater osteoclast numbers. AD–CKD recapitulated key aspects reported in CKD patients and revealed new insights into CKD‐associated oral defects. This model has potential for studying mechanisms of dentoalveolar defects or therapeutic interventions. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

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New animal model of extrinsic dental erosion-Erosive effect on the mouse molar teeth

Abstract: This new animal model of extrinsic dental erosion and the presented method with ground molars observed in SEM are suitable for further studies, which will gain deeper insights into the erosive disease.

Cited by 7 publications

References 47 publications

A review of recent advances in tribology

A review of recent advances in tribology

Dental erosion in mice with impaired salivary gland function

Dentoalveolar Alterations in an Adenine‐Induced Chronic Kidney Disease Mouse Model

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