New and Simple HPLC Method for the Determination of Lactic Acid Content in Ciprofloxacin Injection

McHugh, John; Dumont, Suzanne N.; Paradis, Julie; Nguyen, Anh Loan; Levesque, Suzanne; Carrier, Alain

doi:10.1080/10826070600757789

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…The filtrate was centrifuged at 14,400 g for 15 min and the supernatant was further filtered through 0.45 μm membrane filter. LA in the filtrate was determined by the method of McHugh et al (2006) in an HPLC system (Waters) using a 4.6 9 250 mm, 5 μm, column (ZORBAX Rx-C18; Agilent Technologies), a Waters 2996 photodiode array UV detector programmed at 210 nm and a mixture of acetonitrile:buffer (3:97) as the mobile phase with a flow rate of 1.2 ml/min. The buffer used was 25 mM KH 2 PO 4 (adjusted to pH 2.5 by H 3 PO 4 ).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Application of Fermentation Technology to Use Slaughterhouse Blood as Potential Protein Supplement in Fish Feed

Samaddar¹

2015

JJBS

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Slaughterhouse Blood (SHB) is a rich source of protein, but it requires a suitable processing before its use as an ingredient in animal feed formulations. In this study, SHB was fermented using whey or pure culture of Lactobacillus acidophilus as the fermentation inoculums. Mixtures of SHB, fermentation inoculums and molasses were incubated at 35°C and microbiological and biochemical changes were determined every day until the end of fermentation. The results revealed that the fermentation of SHB by both the inoculums required 4 days to complete. The harmful microorganisms were removed or reduced to a negligible level after fermentation. Both the fermented products were also aerobically stable. Protein, lipid and essential amino acid contents of SHB were almost completely recovered after fermentation. The L. acidophilus fermented SHB (LFSHB) exhibited a better microbiological quality than the whey fermented SHB (WFSHB), while WFSHB exhibited a higher amount of free amino acid than the LFSHB. The present study concludes that fermentation is an appropriate technique to convert SHB into a nutritionally sound and microbiologically safe ingredient for the use in fish feed formulation. Whey can be used as an easily available and cost effective inoculum to carry out the fermentation.

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Section: Biochemical Analysesmentioning

confidence: 99%

Application of Fermentation Technology to Use Slaughterhouse Blood as Potential Protein Supplement in Fish Feed

Samaddar¹

2015

JJBS

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“…The filtrate was centrifuged at 14,400g for 15 min and the supernatant was further filtered through 0.45 lm membrane filter. LA in the filtrate was determined by the method of McHugh et al (2006) in an HPLC system (Waters) using a 4.6 9 250 mm, 5 lm, column (ZORBAX Rx-C18; Agilent Technologies), a Waters 2996 photodiode array UV detector, programmed at 210 nm and a mixture of acetonitrile:buffer (3:97) as the mobile phase with a flow rate of 1.2 ml/min. The buffer used was 25 mM KH 2 PO 4 (adjusted to pH 2.5 by H 3 PO 4 ).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Processing of fish offal waste through fermentation utilizing whey as inoculum

Samaddar

Kaviraj

2014

Int J Recycl Org Waste Agricult

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Effort to minimize the environmental pollution is using that waste as a base material of organic fertilizer. This study was conducted to determine the levels of nitrogen (N) and phosphorus (P) contained in the offal waste of mujair fish (Oreochromis mosambicus) from Lindu lake. Samples of mujair offal waste were taken from fish saler on the street Towua, Palu. Analysis of Nitrogen levels used Kjeldhal method which consists of three phases, namely destruction, distillation and titration. On the other hand, for phosphorus analysis was using wet digestion method, followed by absorbance measurements of samples with spectronic 20 at a wavelength of 720 nm. Based on the analysis, the average of nitrogen (N) levels in mujair offal wastewas 2.78%, while the average of phosphorus (P) levels was 2.48%. From these results, it is known that mujair offal waste contained more nitrogen (N) compared to phosphrus (P). In addition, when compared to the quality standard liquid organic fertilizer N and P content in mujair offal almost meets the quality standard liquid fertilizer, where the minimum technical requirements of organic liquid fertilizer should contain a total N content of 3-6% and 3-6% P2O5.

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“…Lactic acid (LA) is a 2-hydroxypropionic acid with a wide variety of industrial and biotechnological applications [ 1 , 2 , 3 , 4 , 5 , 6 ]. LA has negative impacts on animal metabolisms [ 4 ] and on the growth of Lactic Acid Bacteria (LAB) in dairy technology being produced by anaerobic and O 2 -poor fermentation broths [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Clay-Catalyzed Ozonation of Hydrotalcite-Extracted Lactic Acid Potential Application for Preventing Milk Fermentation Inhibition

Baktaoui

Hadj-Abdelkader²,

Benghaffour³

et al. 2022

Molecules

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An unprecedented route for mitigating the inhibitory effect of lactic acid (LA) on milk fermentation was achieved through lactate adsorption on hydrotalcite (Ht) from simulated lactate extracts. During its regeneration by ozonation, Ht displayed catalytic activity that appeared to increase by addition of montmorillonite (Mt). Changes in the pH, Zeta potential and catalyst particle size during LA ozonation were found to strongly influence LA–LA, LA–catalyst and catalyst–catalyst interactions. The latter determine lactate protonation–deprotonation and clay dispersion in aqueous media. The activity of Mt appears to involve hydrophobic adsorption of non-dissociated LA molecules on silica-rich areas at low pH, and Lewis acid–base and electrostatic interactions at higher pH than the pKa. Hydrotalcite promotes both hydrophobic interaction and anion exchange. Hydrotalcite–smectite mixture was found to enhance clay dispersion and catalytic activity. This research allowed demonstrating that natural clay minerals can act both as adsorbents for LA extract from fermentation broths and as catalysts for adsorbent regeneration. The results obtained herein provide valuable and useful findings for envisaging seed-free milk clotting in dairy technologies.

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New and Simple HPLC Method for the Determination of Lactic Acid Content in Ciprofloxacin Injection

Cited by 6 publications

References 8 publications

Application of Fermentation Technology to Use Slaughterhouse Blood as Potential Protein Supplement in Fish Feed

Application of Fermentation Technology to Use Slaughterhouse Blood as Potential Protein Supplement in Fish Feed

Processing of fish offal waste through fermentation utilizing whey as inoculum

Clay-Catalyzed Ozonation of Hydrotalcite-Extracted Lactic Acid Potential Application for Preventing Milk Fermentation Inhibition

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