New and Old Osteocytic Cell Lines and 3D Models

Pajevic, Paola Divieti

doi:10.1007/s11914-020-00613-3

Cited by 8 publications

(12 citation statements)

References 72 publications

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“…Recently, more evidence has accumulated for the role of osteocytes in bone signalling [ 36 ], and their transcriptome signature is being deciphered [ 57 ] to understand the role of these cells in skeletal homeostasis and disease. Simultaneously, 3D culture systems (including IDG-SW3 cells) are being developed to examine particular points in vitro, which would overcome certain difficulties with the isolation, culturing, and differentiation of osteocytes [ 18 ]. Such an in vitro system would also allow for better extrapolation to the in vivo situation [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, there is a lack of evidence about the effects of FasL on osteocytes. This might be due to difficult access to these cells in vivo compared with osteoblasts and osteoclasts, along with the very limited repertoire of osteocytic cell lines and the demands of handling these in vitro [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…One of the available osteocytic cell lines is IDG-SW3, considered to be the best model to work with mature Sost-positive osteocytes [ 18 ]. In this research, advantage was taken of this system to test the impact of FasL stimulation on the osteogenic profile of the IDG-SW3 osteocytes.…”

Section: Introductionmentioning

confidence: 99%

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Impact of FasL Stimulation on Sclerostin Expression and Osteogenic Profile in IDG-SW3 Osteocytes

Kratochvílová

Ramesova

Veselá

et al. 2021

Biology

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The Fas ligand (FasL) is known from programmed cell death, the immune system, and recently also from bone homeostasis. As such, Fas signalling is a potential target of anti-osteoporotic treatment based on the induction of osteoclastic cell death. Less attention has been paid to osteocytes, although they represent the majority of cells within the mature bone and are the key regulators. To determine the impact of FasL stimulation on osteocytes, differentiated IDG-SW3 cells were challenged by FasL, and their osteogenic expression profiles were evaluated by a pre-designed PCR array. Notably, the most downregulated gene was the one for sclerostin, which is the major marker of osteocytes and a negative regulator of bone formation. FasL stimulation also led to significant changes (over 10-fold) in the expression of other osteogenic markers: Gdf10, Gli1, Ihh, Mmp10, and Phex. To determine whether these alterations involved caspase-dependent or caspase-independent mechanisms, the IDG-SW3 cells were stimulated by FasL with and without a caspase inhibitor: Q-VD-OPh. The alterations were also detected in the samples treated by FasL along with Q-VD-OPh, pointing to the caspase-independent impact of FasL stimulation. These results contribute to an understanding of the recently emerging pleiotropic effects of Fas/FasL signalling and specify its functions in bone cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impact of FasL Stimulation on Sclerostin Expression and Osteogenic Profile in IDG-SW3 Osteocytes

Kratochvílová

Ramesova

Veselá

et al. 2021

Biology

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“…These cells control both osteoblastic bone‐forming and osteoclastic bone‐resorbing activities through secreted factors, notably sclerostin and receptor activator of nuclear factor kappa‐β ligand (RANKL), which suppresses bone formation and promotes bone resorption, respectively 13,14 . However, their relative inaccessibility,until recently a lack of established in vitro cell lines, and in vivo models have impeded progress in understanding their functional roles 15,16 . We have previously established a novel murine osteocytic cell line, Ocy454, that has demonstrated promise as a tool for mechanical transduction studies in 1G and simulated µG studies 17‐19 …”

Section: Introductionmentioning

confidence: 99%

Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight

et al. 2021

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Bone loss is a major health concern for astronauts during long-term spaceflight and for patients during prolonged bed rest or paralysis. Growing evidence suggests that osteocytes, the most abundant cells in the mineralized bone matrix, play a key role in sensing mechanical forces applied to the skeleton and integrating the orchestrated response into subcellular biochemical signals to modulate bone homeostasis. However, the precise molecular mechanisms underlying both mechanosensation and mechanotransduction in late-osteoblast-to-osteocyte cells under microgravity (µG) have yet to be elucidated. To unravel the mechanisms by which late osteoblasts and osteocytes sense and respond to mechanical unloading, we exposed the osteocytic cell line, Ocy454, to 2, 4, or 6 days of µG on the SpaceX Dragon-6 resupply mission to the International Space Station. Our results showed that µG impairs the differentiation of osteocytes, consistent with prior osteoblast spaceflight experiments, which resulted in the downregulation of key osteocytic genes. Importantly, we demonstrate the modulation of critical glycolysis pathways in osteocytes subjected to microgravity and discovered a set of mechanical sensitive genes that are consistently regulated in multiple cell types exposed to microgravity suggesting a common, yet to be fully elucidated, genome-wide response to microgravity. Ground-based simulated microgravity 2 of 18 | UDA et Al.

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“…To reach this objective, PRP has been characterized in terms of: platelet count; fibrinogen and GFs content; effect on in vitro proliferation and differentiation of osteoblast cells. PRP units, obtained by apheresis from four donors, were characterized and tested at different concentrations on HOB-01-C1 [27,28] cell cultures, a pre-osteocytic cell-line, to evaluate their effects on cell viability, mineralization, and differentiation markers over time.…”

Section: Introductionmentioning

confidence: 99%

Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

Pulcini

Merolle

Chiara

et al. 2021

IJMS

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Background: Platelet-Rich Plasma (PRP) induces bone regeneration; however, there is low evidence supporting its efficacy in bone healing. The lack of a standardized protocol of administration represents the main obstacle to its use in the clinical routine for bone defects’ treatment. The purpose of this study was to characterize PRP and elucidate its osteogenic potential. Methods: Platelet count, fibrinogen levels, and growth factors concentration were measured in PRP obtained by four apheresis procedures. HOB-01-C1, a pre-osteocytic cell line, was used to examine the effects of different PRP dilutions (from 1% to 50%) on cell viability, growth, and differentiation. Gene expression of RUNX2, PHEX, COL1A1, and OCN was also assayed. Results: PRP showed a mean 4.6-fold increase of platelets amount compared to whole blood. Among the 36 proteins evaluated, we found the highest concentrations for PDGF isoforms, EGF, TGF-β and VEGF-D. PDGF-AA positively correlated with platelet counts. In three of the four tested units, 25% PRP induced a growth rate comparable to the positive control (10% FBS); whereas, for all the tested units, 10% PRP treatment sustained differentiation. Conclusions: This study showed that PRP from apheresis stimulates proliferation and differentiation of pre-osteocyte cells through the release of growth factors from platelets.

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New and Old Osteocytic Cell Lines and 3D Models

Cited by 8 publications

References 72 publications

Impact of FasL Stimulation on Sclerostin Expression and Osteogenic Profile in IDG-SW3 Osteocytes

Impact of FasL Stimulation on Sclerostin Expression and Osteogenic Profile in IDG-SW3 Osteocytes

Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight

Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

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